Assessment of a multiplex detection method for Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in cow milk

Raw cow milk is considered one of the most important vehicles for pathogenic bacteria like Salmonella spp., Escherichia coli O157:H7, and Listeria monocytogenes. These three bacteria are responsible for foodborne diseases. Routine microbiological methods to detect these microorganisms in cow mil...

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Autor principal:	Rocio Patiño Burbano, Ana Karina Carrasca, Jorge Luis Parra Arango, Jorge Luis Parra Arango
Formato:	article
Lenguaje:	EN ES
Publicado:	Pontificia Universidad Javeriana 2019
Materias:	foodborne diseases; food safety; hygienic quality of milk. Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/402e5767a81d43f6bca21d4ebe8ae029
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spelling	oai:doaj.org-article:402e5767a81d43f6bca21d4ebe8ae0292021-11-16T15:04:18ZAssessment of a multiplex detection method for Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in cow milk10.11144/Javeriana.SC24-1.aoam0122-74832027-1352https://doaj.org/article/402e5767a81d43f6bca21d4ebe8ae0292019-04-01T00:00:00Zhttps://revistas.javeriana.edu.co/index.php/scientarium/article/view/20519https://doaj.org/toc/0122-7483https://doaj.org/toc/2027-1352Raw cow milk is considered one of the most important vehicles for pathogenic bacteria like Salmonella spp., Escherichia coli O157:H7, and Listeria monocytogenes. These three bacteria are responsible for foodborne diseases. Routine microbiological methods to detect these microorganisms in cow milk can be complicated and time consuming. The aim of this work was to evaluate a method to simultaneously detect Salmonella spp., Escherichia coli O157:H7, and Listeria monocytogenes in experimentally contaminated cow milk. The assessed method combined a standard microbiological culture step, using a pre-enrichment medium that favors the growth of the three focal microorganisms: SEL broth, followed by a single PCR assay. A total of 43 interference bacterial strains were used to evaluate the method’s specificity. The detection rate for the microbiological method with standard culture media was 10 UFC/mL, and that of the PCR detection, following pre-enrichment in SEL broth, was 10 UFC/mL for S. enterica and L. monocytogenes and between 1 and 5 UFC/mL for E. coli O157:H7. The PCR method showed specificity for the reference strains. Simultaneous detection by multiple PCR using SEL broth was successful for the detection of S. enterica, E. coli O157:H7, and L. monocytogenes in samples of experimentally contaminated cow milk, featuring both a high detection rate and a high specificity. This approach promises to be a feasible routine procedure when testing milk samples in industry and public health control setups.Rocio Patiño Burbano, Ana Karina Carrasca, Jorge Luis Parra Arango, Jorge Luis Parra ArangoPontificia Universidad Javerianaarticlefoodborne diseases; food safety; hygienic quality of milk.Science (General)Q1-390ENESUniversitas Scientiarum, Vol 24, Iss 1, Pp 277-294 (2019)
institution	DOAJ
collection	DOAJ
language	EN ES
topic	foodborne diseases; food safety; hygienic quality of milk. Science (General) Q1-390
spellingShingle	foodborne diseases; food safety; hygienic quality of milk. Science (General) Q1-390 Rocio Patiño Burbano, Ana Karina Carrasca, Jorge Luis Parra Arango, Jorge Luis Parra Arango Assessment of a multiplex detection method for Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in cow milk
description	Raw cow milk is considered one of the most important vehicles for pathogenic bacteria like Salmonella spp., Escherichia coli O157:H7, and Listeria monocytogenes. These three bacteria are responsible for foodborne diseases. Routine microbiological methods to detect these microorganisms in cow milk can be complicated and time consuming. The aim of this work was to evaluate a method to simultaneously detect Salmonella spp., Escherichia coli O157:H7, and Listeria monocytogenes in experimentally contaminated cow milk. The assessed method combined a standard microbiological culture step, using a pre-enrichment medium that favors the growth of the three focal microorganisms: SEL broth, followed by a single PCR assay. A total of 43 interference bacterial strains were used to evaluate the method’s specificity. The detection rate for the microbiological method with standard culture media was 10 UFC/mL, and that of the PCR detection, following pre-enrichment in SEL broth, was 10 UFC/mL for S. enterica and L. monocytogenes and between 1 and 5 UFC/mL for E. coli O157:H7. The PCR method showed specificity for the reference strains. Simultaneous detection by multiple PCR using SEL broth was successful for the detection of S. enterica, E. coli O157:H7, and L. monocytogenes in samples of experimentally contaminated cow milk, featuring both a high detection rate and a high specificity. This approach promises to be a feasible routine procedure when testing milk samples in industry and public health control setups.
format	article
author	Rocio Patiño Burbano, Ana Karina Carrasca, Jorge Luis Parra Arango, Jorge Luis Parra Arango
author_facet	Rocio Patiño Burbano, Ana Karina Carrasca, Jorge Luis Parra Arango, Jorge Luis Parra Arango
author_sort	Rocio Patiño Burbano, Ana Karina Carrasca, Jorge Luis Parra Arango, Jorge Luis Parra Arango
title	Assessment of a multiplex detection method for Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in cow milk
title_short	Assessment of a multiplex detection method for Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in cow milk
title_full	Assessment of a multiplex detection method for Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in cow milk
title_fullStr	Assessment of a multiplex detection method for Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in cow milk
title_full_unstemmed	Assessment of a multiplex detection method for Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in cow milk
title_sort	assessment of a multiplex detection method for salmonella enterica, escherichia coli o157:h7, and listeria monocytogenes in cow milk
publisher	Pontificia Universidad Javeriana
publishDate	2019
url	https://doaj.org/article/402e5767a81d43f6bca21d4ebe8ae029
work_keys_str_mv	AT rociopatinoburbanoanakarinacarrascajorgeluisparraarangojorgeluisparraarango assessmentofamultiplexdetectionmethodforsalmonellaentericaescherichiacolio157h7andlisteriamonocytogenesincowmilk
_version_	1718426351511273472

Assessment of a multiplex detection method for Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in cow milk

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