Antimicrobially Active Semen Extenders Allow the Reduction of Antibiotic Use in Pig Insemination

Antibiotic use in semen extenders for livestock may contribute to the development and spreading of multi-drug resistance. Antimicrobial control in semen doses for artificial insemination of pigs is indispensable due to the relatively high storage temperature (17 °C). The objectives of this study wer...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Anne-Marie Luther, Thu Quynh Nguyen, Jutta Verspohl, Dagmar Waberski
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	boar spermatozoa semen preservation antibiotics semen extender Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/40307a0e77b94d9ea381563d01b899f0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:40307a0e77b94d9ea381563d01b899f0
record_format	dspace
spelling	oai:doaj.org-article:40307a0e77b94d9ea381563d01b899f02021-11-25T16:22:45ZAntimicrobially Active Semen Extenders Allow the Reduction of Antibiotic Use in Pig Insemination10.3390/antibiotics101113192079-6382https://doaj.org/article/40307a0e77b94d9ea381563d01b899f02021-10-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1319https://doaj.org/toc/2079-6382Antibiotic use in semen extenders for livestock may contribute to the development and spreading of multi-drug resistance. Antimicrobial control in semen doses for artificial insemination of pigs is indispensable due to the relatively high storage temperature (17 °C). The objectives of this study were first, to examine whether the antimicrobial capacity differs between antibiotic-free extenders and second, to determine whether an antimicrobial active extender provides the possibility to reduce antibiotics. Antibiotic-free semen extenders Beltsville Thawing Solution (BTS) and Androstar Premium were inoculated at 10<sup>3</sup> to 10<sup>4</sup> CFU/mL with four pure bacterial strains isolated from boar ejaculates or a mixture thereof, and then stored for 144 h at 17 °C. Bacterial counts after aerobic culture decreased in BTS up to one log level and decreased in Androstar Premium by 2 to 3.5 log levels (<i>p</i> < 0.05). In semen samples from nine boars stored in the inoculated Androstar Premium extender containing half of the standard concentration of gentamicin, bacteria counts were below 10<sup>1</sup> CFU/mL. Likewise, half of the standard dose of apramycin and ampicillin was fully antimicrobially active and sperm quality was maintained. In conclusion, semen extenders with intrinsic antimicrobial activity allow a reduction in antibiotic use in pig insemination.Anne-Marie LutherThu Quynh NguyenJutta VerspohlDagmar WaberskiMDPI AGarticleboarspermatozoasemen preservationantibioticssemen extenderTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1319, p 1319 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	boar spermatozoa semen preservation antibiotics semen extender Therapeutics. Pharmacology RM1-950
spellingShingle	boar spermatozoa semen preservation antibiotics semen extender Therapeutics. Pharmacology RM1-950 Anne-Marie Luther Thu Quynh Nguyen Jutta Verspohl Dagmar Waberski Antimicrobially Active Semen Extenders Allow the Reduction of Antibiotic Use in Pig Insemination
description	Antibiotic use in semen extenders for livestock may contribute to the development and spreading of multi-drug resistance. Antimicrobial control in semen doses for artificial insemination of pigs is indispensable due to the relatively high storage temperature (17 °C). The objectives of this study were first, to examine whether the antimicrobial capacity differs between antibiotic-free extenders and second, to determine whether an antimicrobial active extender provides the possibility to reduce antibiotics. Antibiotic-free semen extenders Beltsville Thawing Solution (BTS) and Androstar Premium were inoculated at 10<sup>3</sup> to 10<sup>4</sup> CFU/mL with four pure bacterial strains isolated from boar ejaculates or a mixture thereof, and then stored for 144 h at 17 °C. Bacterial counts after aerobic culture decreased in BTS up to one log level and decreased in Androstar Premium by 2 to 3.5 log levels (<i>p</i> < 0.05). In semen samples from nine boars stored in the inoculated Androstar Premium extender containing half of the standard concentration of gentamicin, bacteria counts were below 10<sup>1</sup> CFU/mL. Likewise, half of the standard dose of apramycin and ampicillin was fully antimicrobially active and sperm quality was maintained. In conclusion, semen extenders with intrinsic antimicrobial activity allow a reduction in antibiotic use in pig insemination.
format	article
author	Anne-Marie Luther Thu Quynh Nguyen Jutta Verspohl Dagmar Waberski
author_facet	Anne-Marie Luther Thu Quynh Nguyen Jutta Verspohl Dagmar Waberski
author_sort	Anne-Marie Luther
title	Antimicrobially Active Semen Extenders Allow the Reduction of Antibiotic Use in Pig Insemination
title_short	Antimicrobially Active Semen Extenders Allow the Reduction of Antibiotic Use in Pig Insemination
title_full	Antimicrobially Active Semen Extenders Allow the Reduction of Antibiotic Use in Pig Insemination
title_fullStr	Antimicrobially Active Semen Extenders Allow the Reduction of Antibiotic Use in Pig Insemination
title_full_unstemmed	Antimicrobially Active Semen Extenders Allow the Reduction of Antibiotic Use in Pig Insemination
title_sort	antimicrobially active semen extenders allow the reduction of antibiotic use in pig insemination
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/40307a0e77b94d9ea381563d01b899f0
work_keys_str_mv	AT annemarieluther antimicrobiallyactivesemenextendersallowthereductionofantibioticuseinpiginsemination AT thuquynhnguyen antimicrobiallyactivesemenextendersallowthereductionofantibioticuseinpiginsemination AT juttaverspohl antimicrobiallyactivesemenextendersallowthereductionofantibioticuseinpiginsemination AT dagmarwaberski antimicrobiallyactivesemenextendersallowthereductionofantibioticuseinpiginsemination
_version_	1718413194151591936

Antimicrobially Active Semen Extenders Allow the Reduction of Antibiotic Use in Pig Insemination

Ejemplares similares