Promotion of biological nitrogen fixation activity of an anaerobic consortium using humin as an extracellular electron mediator

Abstract Nitrogen fertiliser is manufactured using the industrial Haber–Bosch process, although it is extremely energy-consuming. One sustainable alternative technology is the electrochemical promotion of biological nitrogen fixation (BNF). This study reports the promotion of BNF activity of anaerob...

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Autores principales:	Sujan Dey, Takanori Awata, Jumpei Mitsushita, Dongdong Zhang, Takuya Kasai, Norihisa Matsuura, Arata Katayama
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/403427e90eb6413eb651f6438067c0a1
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spelling	oai:doaj.org-article:403427e90eb6413eb651f6438067c0a12021-12-02T17:04:06ZPromotion of biological nitrogen fixation activity of an anaerobic consortium using humin as an extracellular electron mediator10.1038/s41598-021-85955-32045-2322https://doaj.org/article/403427e90eb6413eb651f6438067c0a12021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85955-3https://doaj.org/toc/2045-2322Abstract Nitrogen fertiliser is manufactured using the industrial Haber–Bosch process, although it is extremely energy-consuming. One sustainable alternative technology is the electrochemical promotion of biological nitrogen fixation (BNF). This study reports the promotion of BNF activity of anaerobic microbial consortia by humin, a solid-phase humic substance, at any pH, functioning as an extracellular electron mediator, to levels of 5.7–11.8 times under nitrogen-deficient conditions. This was evidenced by increased acetylene reduction activity and total nitrogen content of the consortia. Various humins from different origins promoted anaerobic BNF activity, although the degree of promotion differed. The promotion effected by humin differed from the effects of chemical reducing agents and the effects of supplemental micronutrients and vitamins. The promotion of anaerobic BNF activity by only reduced humin without any other electron donor suggested that humin did not serve as organic carbon source but as extracellular electron mediator, for electron donation to the nitrogen-fixing microorganisms. The next generation sequencing (NGS) of partial 16S rRNA genes showed the predominance of Clostridiales (Firmicutes) in the consortia. These findings suggest the effectiveness of humin as a solid-phase extracellular electron mediator for the promotion of anaerobic BNF activity, potentially to serve for the basis for a sustainable technology.Sujan DeyTakanori AwataJumpei MitsushitaDongdong ZhangTakuya KasaiNorihisa MatsuuraArata KatayamaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sujan Dey Takanori Awata Jumpei Mitsushita Dongdong Zhang Takuya Kasai Norihisa Matsuura Arata Katayama Promotion of biological nitrogen fixation activity of an anaerobic consortium using humin as an extracellular electron mediator
description	Abstract Nitrogen fertiliser is manufactured using the industrial Haber–Bosch process, although it is extremely energy-consuming. One sustainable alternative technology is the electrochemical promotion of biological nitrogen fixation (BNF). This study reports the promotion of BNF activity of anaerobic microbial consortia by humin, a solid-phase humic substance, at any pH, functioning as an extracellular electron mediator, to levels of 5.7–11.8 times under nitrogen-deficient conditions. This was evidenced by increased acetylene reduction activity and total nitrogen content of the consortia. Various humins from different origins promoted anaerobic BNF activity, although the degree of promotion differed. The promotion effected by humin differed from the effects of chemical reducing agents and the effects of supplemental micronutrients and vitamins. The promotion of anaerobic BNF activity by only reduced humin without any other electron donor suggested that humin did not serve as organic carbon source but as extracellular electron mediator, for electron donation to the nitrogen-fixing microorganisms. The next generation sequencing (NGS) of partial 16S rRNA genes showed the predominance of Clostridiales (Firmicutes) in the consortia. These findings suggest the effectiveness of humin as a solid-phase extracellular electron mediator for the promotion of anaerobic BNF activity, potentially to serve for the basis for a sustainable technology.
format	article
author	Sujan Dey Takanori Awata Jumpei Mitsushita Dongdong Zhang Takuya Kasai Norihisa Matsuura Arata Katayama
author_facet	Sujan Dey Takanori Awata Jumpei Mitsushita Dongdong Zhang Takuya Kasai Norihisa Matsuura Arata Katayama
author_sort	Sujan Dey
title	Promotion of biological nitrogen fixation activity of an anaerobic consortium using humin as an extracellular electron mediator
title_short	Promotion of biological nitrogen fixation activity of an anaerobic consortium using humin as an extracellular electron mediator
title_full	Promotion of biological nitrogen fixation activity of an anaerobic consortium using humin as an extracellular electron mediator
title_fullStr	Promotion of biological nitrogen fixation activity of an anaerobic consortium using humin as an extracellular electron mediator
title_full_unstemmed	Promotion of biological nitrogen fixation activity of an anaerobic consortium using humin as an extracellular electron mediator
title_sort	promotion of biological nitrogen fixation activity of an anaerobic consortium using humin as an extracellular electron mediator
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/403427e90eb6413eb651f6438067c0a1
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Promotion of biological nitrogen fixation activity of an anaerobic consortium using humin as an extracellular electron mediator

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