Role of the stem cell-associated intermediate filament nestin in malignant proliferation of non-small cell lung cancer.
<h4>Background</h4>Nestin is associated with neoplastic transformation, but the mechanisms by which nestin contributes to invasion and malignancy of lung cancer remain unknown. Considering that proliferation is necessary for malignant behavior, we investigated the mechanism of nestin act...
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2014
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oai:doaj.org-article:4049ff492ee94718a88f747c6257b71e2021-11-18T08:34:22ZRole of the stem cell-associated intermediate filament nestin in malignant proliferation of non-small cell lung cancer.1932-620310.1371/journal.pone.0085584https://doaj.org/article/4049ff492ee94718a88f747c6257b71e2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24498263/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Nestin is associated with neoplastic transformation, but the mechanisms by which nestin contributes to invasion and malignancy of lung cancer remain unknown. Considering that proliferation is necessary for malignant behavior, we investigated the mechanism of nestin action in association with the proliferative properties of non-small cell lung cancer (NSCLC).<h4>Methods</h4>Nestin expression was examined in NSCLC specimens and cell lines. Associations with clinicopathological features, including prognosis and proliferative markers, were evaluated. Effects of nestin knockdown on proliferation and the signaling pathways involved were further investigated.<h4>Results</h4>Nestin was expressed in most cancer specimens and all the tumor cell lines analyzed. High nestin expression in malignant tissue was associated with high Ki-67 or PCNA levels and poor patient outcomes. Conversely, knockdown of nestin expression led to significant inhibition of tumor cell proliferation, decreased colony forming ability, and cell cycle G1 arrest. Furthermore, nestin knockdown resulted in inhibition of Akt and GSK3β activation.<h4>Conclusions</h4>Our data demonstrate that nestin expression in NSCLC cells is associated with poor prognosis of patients and tumor cell proliferation pathway. Downregulation of nestin efficiently inhibited lung cancer cell proliferation, which might be through affecting cell cycle arrest and Akt-GSK3β-Rb signaling pathway.Zhenguang ChenJiancheng WangLie CaiBeilong ZhongHonghe LuoYuantao HaoWeihua YuBinchao WangChunhua SuYiyan LeiAmos Ela BellaAndy Peng XiangTao WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e85584 (2014) |
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Medicine R Science Q Zhenguang Chen Jiancheng Wang Lie Cai Beilong Zhong Honghe Luo Yuantao Hao Weihua Yu Binchao Wang Chunhua Su Yiyan Lei Amos Ela Bella Andy Peng Xiang Tao Wang Role of the stem cell-associated intermediate filament nestin in malignant proliferation of non-small cell lung cancer. |
| description |
<h4>Background</h4>Nestin is associated with neoplastic transformation, but the mechanisms by which nestin contributes to invasion and malignancy of lung cancer remain unknown. Considering that proliferation is necessary for malignant behavior, we investigated the mechanism of nestin action in association with the proliferative properties of non-small cell lung cancer (NSCLC).<h4>Methods</h4>Nestin expression was examined in NSCLC specimens and cell lines. Associations with clinicopathological features, including prognosis and proliferative markers, were evaluated. Effects of nestin knockdown on proliferation and the signaling pathways involved were further investigated.<h4>Results</h4>Nestin was expressed in most cancer specimens and all the tumor cell lines analyzed. High nestin expression in malignant tissue was associated with high Ki-67 or PCNA levels and poor patient outcomes. Conversely, knockdown of nestin expression led to significant inhibition of tumor cell proliferation, decreased colony forming ability, and cell cycle G1 arrest. Furthermore, nestin knockdown resulted in inhibition of Akt and GSK3β activation.<h4>Conclusions</h4>Our data demonstrate that nestin expression in NSCLC cells is associated with poor prognosis of patients and tumor cell proliferation pathway. Downregulation of nestin efficiently inhibited lung cancer cell proliferation, which might be through affecting cell cycle arrest and Akt-GSK3β-Rb signaling pathway. |
| format |
article |
| author |
Zhenguang Chen Jiancheng Wang Lie Cai Beilong Zhong Honghe Luo Yuantao Hao Weihua Yu Binchao Wang Chunhua Su Yiyan Lei Amos Ela Bella Andy Peng Xiang Tao Wang |
| author_facet |
Zhenguang Chen Jiancheng Wang Lie Cai Beilong Zhong Honghe Luo Yuantao Hao Weihua Yu Binchao Wang Chunhua Su Yiyan Lei Amos Ela Bella Andy Peng Xiang Tao Wang |
| author_sort |
Zhenguang Chen |
| title |
Role of the stem cell-associated intermediate filament nestin in malignant proliferation of non-small cell lung cancer. |
| title_short |
Role of the stem cell-associated intermediate filament nestin in malignant proliferation of non-small cell lung cancer. |
| title_full |
Role of the stem cell-associated intermediate filament nestin in malignant proliferation of non-small cell lung cancer. |
| title_fullStr |
Role of the stem cell-associated intermediate filament nestin in malignant proliferation of non-small cell lung cancer. |
| title_full_unstemmed |
Role of the stem cell-associated intermediate filament nestin in malignant proliferation of non-small cell lung cancer. |
| title_sort |
role of the stem cell-associated intermediate filament nestin in malignant proliferation of non-small cell lung cancer. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/4049ff492ee94718a88f747c6257b71e |
| work_keys_str_mv |
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