Differentially expressed genes in Bordetella pertussis strains belonging to a lineage which recently spread globally.

Pertussis is a highly contagious, acute respiratory disease in humans caused by the Gram-negative pathogen Bordetella pertussis. Pertussis has resurged in the face of intensive vaccination and this has coincided with the emergence of strains carrying a particular allele for the pertussis toxin promo...

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Autores principales:	Daan de Gouw, Peter W M Hermans, Hester J Bootsma, Aldert Zomer, Kees Heuvelman, Dimitri A Diavatopoulos, Frits R Mooi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/404c754176dd414c8db036dcb669f35f
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spelling	oai:doaj.org-article:404c754176dd414c8db036dcb669f35f2021-11-18T08:38:33ZDifferentially expressed genes in Bordetella pertussis strains belonging to a lineage which recently spread globally.1932-620310.1371/journal.pone.0084523https://doaj.org/article/404c754176dd414c8db036dcb669f35f2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24416242/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Pertussis is a highly contagious, acute respiratory disease in humans caused by the Gram-negative pathogen Bordetella pertussis. Pertussis has resurged in the face of intensive vaccination and this has coincided with the emergence of strains carrying a particular allele for the pertussis toxin promoter, ptxP3, which is associated with higher levels of pertussis toxin (Ptx) production. Within 10 to 20 years, ptxP3 strains have nearly completely replaced the previously dominant ptxP1 strains resulting in a worldwide selective sweep. In order to identify B. pertussis genes associated with the selective sweep, we compared the expression of genes in ptxP1 and ptxP3 strains that are under control of the Bordetella master virulence regulatory locus (bvgASR). The BvgAS proteins comprise a two component sensory transduction system which is regulated by temperature, nicotinic acid and sulfate. By increasing the sulfate concentration, it is possible to change the phase of B. pertussis from virulent to avirulent. Until recently, the only distinctive phenotype of ptxP3 strains was a higher Ptx production. Here we identify additional phenotypic differences between ptxP1 and ptxP3 strains which may have contributed to its global spread by comparing global transcriptional responses under sulfate-modulating conditions. We show that ptxP3 strains are less sensitive to sulfate-mediated gene suppression, resulting in an increased production of the vaccine antigens pertactin (Prn) and Ptx and a number of other virulence genes, including a type III secretion toxin, Vag8, a protein involved in complement resistance, and lpxE involved in lipid A modification. Furthermore, enhanced expression of the vaccine antigens Ptx and Prn by ptxP3 strains was confirmed at the protein level. Identification of genes differentially expressed between ptxP1 and ptxP3 strains may elucidate how B. pertussis has adapted to vaccination and allow the improvement of pertussis vaccines by identifying novel vaccine candidates.Daan de GouwPeter W M HermansHester J BootsmaAldert ZomerKees HeuvelmanDimitri A DiavatopoulosFrits R MooiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e84523 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daan de Gouw Peter W M Hermans Hester J Bootsma Aldert Zomer Kees Heuvelman Dimitri A Diavatopoulos Frits R Mooi Differentially expressed genes in Bordetella pertussis strains belonging to a lineage which recently spread globally.
description	Pertussis is a highly contagious, acute respiratory disease in humans caused by the Gram-negative pathogen Bordetella pertussis. Pertussis has resurged in the face of intensive vaccination and this has coincided with the emergence of strains carrying a particular allele for the pertussis toxin promoter, ptxP3, which is associated with higher levels of pertussis toxin (Ptx) production. Within 10 to 20 years, ptxP3 strains have nearly completely replaced the previously dominant ptxP1 strains resulting in a worldwide selective sweep. In order to identify B. pertussis genes associated with the selective sweep, we compared the expression of genes in ptxP1 and ptxP3 strains that are under control of the Bordetella master virulence regulatory locus (bvgASR). The BvgAS proteins comprise a two component sensory transduction system which is regulated by temperature, nicotinic acid and sulfate. By increasing the sulfate concentration, it is possible to change the phase of B. pertussis from virulent to avirulent. Until recently, the only distinctive phenotype of ptxP3 strains was a higher Ptx production. Here we identify additional phenotypic differences between ptxP1 and ptxP3 strains which may have contributed to its global spread by comparing global transcriptional responses under sulfate-modulating conditions. We show that ptxP3 strains are less sensitive to sulfate-mediated gene suppression, resulting in an increased production of the vaccine antigens pertactin (Prn) and Ptx and a number of other virulence genes, including a type III secretion toxin, Vag8, a protein involved in complement resistance, and lpxE involved in lipid A modification. Furthermore, enhanced expression of the vaccine antigens Ptx and Prn by ptxP3 strains was confirmed at the protein level. Identification of genes differentially expressed between ptxP1 and ptxP3 strains may elucidate how B. pertussis has adapted to vaccination and allow the improvement of pertussis vaccines by identifying novel vaccine candidates.
format	article
author	Daan de Gouw Peter W M Hermans Hester J Bootsma Aldert Zomer Kees Heuvelman Dimitri A Diavatopoulos Frits R Mooi
author_facet	Daan de Gouw Peter W M Hermans Hester J Bootsma Aldert Zomer Kees Heuvelman Dimitri A Diavatopoulos Frits R Mooi
author_sort	Daan de Gouw
title	Differentially expressed genes in Bordetella pertussis strains belonging to a lineage which recently spread globally.
title_short	Differentially expressed genes in Bordetella pertussis strains belonging to a lineage which recently spread globally.
title_full	Differentially expressed genes in Bordetella pertussis strains belonging to a lineage which recently spread globally.
title_fullStr	Differentially expressed genes in Bordetella pertussis strains belonging to a lineage which recently spread globally.
title_full_unstemmed	Differentially expressed genes in Bordetella pertussis strains belonging to a lineage which recently spread globally.
title_sort	differentially expressed genes in bordetella pertussis strains belonging to a lineage which recently spread globally.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/404c754176dd414c8db036dcb669f35f
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Differentially expressed genes in Bordetella pertussis strains belonging to a lineage which recently spread globally.

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