In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution

Adaptive optics (AO) corrects sample aberrations and allows high spatial resolution at depth in vivo. Here the authors report an AO method for Bessel focus; they apply AO Bessel focus scanning fluorescence microscopy to volumetric imaging and measure synaptic calcium and glutamate activity in vivo.

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Autores principales:	Wei Chen, Ryan G. Natan, Yuhan Yang, Shih-Wei Chou, Qinrong Zhang, Ehud Y. Isacoff, Na Ji
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/404cb61605854f9baab64625e7ca5c85
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spelling	oai:doaj.org-article:404cb61605854f9baab64625e7ca5c852021-11-21T12:38:59ZIn vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution10.1038/s41467-021-26965-72041-1723https://doaj.org/article/404cb61605854f9baab64625e7ca5c852021-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-26965-7https://doaj.org/toc/2041-1723Adaptive optics (AO) corrects sample aberrations and allows high spatial resolution at depth in vivo. Here the authors report an AO method for Bessel focus; they apply AO Bessel focus scanning fluorescence microscopy to volumetric imaging and measure synaptic calcium and glutamate activity in vivo.Wei ChenRyan G. NatanYuhan YangShih-Wei ChouQinrong ZhangEhud Y. IsacoffNa JiNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Wei Chen Ryan G. Natan Yuhan Yang Shih-Wei Chou Qinrong Zhang Ehud Y. Isacoff Na Ji In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution
description	Adaptive optics (AO) corrects sample aberrations and allows high spatial resolution at depth in vivo. Here the authors report an AO method for Bessel focus; they apply AO Bessel focus scanning fluorescence microscopy to volumetric imaging and measure synaptic calcium and glutamate activity in vivo.
format	article
author	Wei Chen Ryan G. Natan Yuhan Yang Shih-Wei Chou Qinrong Zhang Ehud Y. Isacoff Na Ji
author_facet	Wei Chen Ryan G. Natan Yuhan Yang Shih-Wei Chou Qinrong Zhang Ehud Y. Isacoff Na Ji
author_sort	Wei Chen
title	In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution
title_short	In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution
title_full	In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution
title_fullStr	In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution
title_full_unstemmed	In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution
title_sort	in vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/404cb61605854f9baab64625e7ca5c85
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In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution

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