In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution

In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution

Adaptive optics (AO) corrects sample aberrations and allows high spatial resolution at depth in vivo. Here the authors report an AO method for Bessel focus; they apply AO Bessel focus scanning fluorescence microscopy to volumetric imaging and measure synaptic calcium and glutamate activity in vivo.

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Autores principales: Wei Chen, Ryan G. Natan, Yuhan Yang, Shih-Wei Chou, Qinrong Zhang, Ehud Y. Isacoff, Na Ji
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
Materias:
Science
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Acceso en línea:https://doaj.org/article/404cb61605854f9baab64625e7ca5c85
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