A multi-platform flow device for microbial (co-) cultivation and microscopic analysis.

Novel microbial cultivation platforms are of increasing interest to researchers in academia and industry. The development of materials with specialized chemical and geometric properties has opened up new possibilities in the study of previously unculturable microorganisms and has facilitated the des...

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Autores principales: Matthijn C Hesselman, Dorett I Odoni, Brendan M Ryback, Suzette de Groot, Ruben G A van Heck, Jaap Keijsers, Pim Kolkman, David Nieuwenhuijse, Youri M van Nuland, Erik Sebus, Rob Spee, Hugo de Vries, Marten T Wapenaar, Colin J Ingham, Karin Schroën, Vítor A P Martins dos Santos, Sebastiaan K Spaans, Floor Hugenholtz, Mark W J van Passel
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Publicado: Public Library of Science (PLoS) 2012
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Acceso en línea:https://doaj.org/article/404dd47b078f4841bdb59d9234fde3f2
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spelling oai:doaj.org-article:404dd47b078f4841bdb59d9234fde3f22021-11-18T07:18:55ZA multi-platform flow device for microbial (co-) cultivation and microscopic analysis.1932-620310.1371/journal.pone.0036982https://doaj.org/article/404dd47b078f4841bdb59d9234fde3f22012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22606321/?tool=EBIhttps://doaj.org/toc/1932-6203Novel microbial cultivation platforms are of increasing interest to researchers in academia and industry. The development of materials with specialized chemical and geometric properties has opened up new possibilities in the study of previously unculturable microorganisms and has facilitated the design of elegant, high-throughput experimental set-ups. Within the context of the international Genetically Engineered Machine (iGEM) competition, we set out to design, manufacture, and implement a flow device that can accommodate multiple growth platforms, that is, a silicon nitride based microsieve and a porous aluminium oxide based microdish. It provides control over (co-)culturing conditions similar to a chemostat, while allowing organisms to be observed microscopically. The device was designed to be affordable, reusable, and above all, versatile. To test its functionality and general utility, we performed multiple experiments with Escherichia coli cells harboring synthetic gene circuits and were able to quantitatively study emerging expression dynamics in real-time via fluorescence microscopy. Furthermore, we demonstrated that the device provides a unique environment for the cultivation of nematodes, suggesting that the device could also prove useful in microscopy studies of multicellular microorganisms.Matthijn C HesselmanDorett I OdoniBrendan M RybackSuzette de GrootRuben G A van HeckJaap KeijsersPim KolkmanDavid NieuwenhuijseYouri M van NulandErik SebusRob SpeeHugo de VriesMarten T WapenaarColin J InghamKarin SchroënVítor A P Martins dos SantosSebastiaan K SpaansFloor HugenholtzMark W J van PasselPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e36982 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Matthijn C Hesselman
Dorett I Odoni
Brendan M Ryback
Suzette de Groot
Ruben G A van Heck
Jaap Keijsers
Pim Kolkman
David Nieuwenhuijse
Youri M van Nuland
Erik Sebus
Rob Spee
Hugo de Vries
Marten T Wapenaar
Colin J Ingham
Karin Schroën
Vítor A P Martins dos Santos
Sebastiaan K Spaans
Floor Hugenholtz
Mark W J van Passel
A multi-platform flow device for microbial (co-) cultivation and microscopic analysis.
description Novel microbial cultivation platforms are of increasing interest to researchers in academia and industry. The development of materials with specialized chemical and geometric properties has opened up new possibilities in the study of previously unculturable microorganisms and has facilitated the design of elegant, high-throughput experimental set-ups. Within the context of the international Genetically Engineered Machine (iGEM) competition, we set out to design, manufacture, and implement a flow device that can accommodate multiple growth platforms, that is, a silicon nitride based microsieve and a porous aluminium oxide based microdish. It provides control over (co-)culturing conditions similar to a chemostat, while allowing organisms to be observed microscopically. The device was designed to be affordable, reusable, and above all, versatile. To test its functionality and general utility, we performed multiple experiments with Escherichia coli cells harboring synthetic gene circuits and were able to quantitatively study emerging expression dynamics in real-time via fluorescence microscopy. Furthermore, we demonstrated that the device provides a unique environment for the cultivation of nematodes, suggesting that the device could also prove useful in microscopy studies of multicellular microorganisms.
format article
author Matthijn C Hesselman
Dorett I Odoni
Brendan M Ryback
Suzette de Groot
Ruben G A van Heck
Jaap Keijsers
Pim Kolkman
David Nieuwenhuijse
Youri M van Nuland
Erik Sebus
Rob Spee
Hugo de Vries
Marten T Wapenaar
Colin J Ingham
Karin Schroën
Vítor A P Martins dos Santos
Sebastiaan K Spaans
Floor Hugenholtz
Mark W J van Passel
author_facet Matthijn C Hesselman
Dorett I Odoni
Brendan M Ryback
Suzette de Groot
Ruben G A van Heck
Jaap Keijsers
Pim Kolkman
David Nieuwenhuijse
Youri M van Nuland
Erik Sebus
Rob Spee
Hugo de Vries
Marten T Wapenaar
Colin J Ingham
Karin Schroën
Vítor A P Martins dos Santos
Sebastiaan K Spaans
Floor Hugenholtz
Mark W J van Passel
author_sort Matthijn C Hesselman
title A multi-platform flow device for microbial (co-) cultivation and microscopic analysis.
title_short A multi-platform flow device for microbial (co-) cultivation and microscopic analysis.
title_full A multi-platform flow device for microbial (co-) cultivation and microscopic analysis.
title_fullStr A multi-platform flow device for microbial (co-) cultivation and microscopic analysis.
title_full_unstemmed A multi-platform flow device for microbial (co-) cultivation and microscopic analysis.
title_sort multi-platform flow device for microbial (co-) cultivation and microscopic analysis.
publisher Public Library of Science (PLoS)
publishDate 2012
url https://doaj.org/article/404dd47b078f4841bdb59d9234fde3f2
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