Comparative analysis of αB-crystallin expression in heat-stressed myocardial cells in vivo and in vitro.

Relationships between αB-crystallin expression patterns and pathological changes of myocardial cells after heat stress were examined in vitro and in vivo in this study using the H9C2 cell line and Sprague-Dawley rats, respectively. Histopathological lesions, characterized by acute degeneration, kary...

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Autores principales:	Shu Tang, Yingjun Lv, Hongbo Chen, Abdelnasir Adam, Yanfen Cheng, Jörg Hartung, Endong Bao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4050d83b38d548f885988ae2f13af0fa
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spelling	oai:doaj.org-article:4050d83b38d548f885988ae2f13af0fa2021-11-18T08:36:18ZComparative analysis of αB-crystallin expression in heat-stressed myocardial cells in vivo and in vitro.1932-620310.1371/journal.pone.0086937https://doaj.org/article/4050d83b38d548f885988ae2f13af0fa2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24466295/?tool=EBIhttps://doaj.org/toc/1932-6203Relationships between αB-crystallin expression patterns and pathological changes of myocardial cells after heat stress were examined in vitro and in vivo in this study using the H9C2 cell line and Sprague-Dawley rats, respectively. Histopathological lesions, characterized by acute degeneration, karyopyknosis and loss of a defined nucleus, became more severe in rat hearts over the course of heat stress treatment from 20 min to 100 min. The expression of αB-crystallin in rat hearts showed a significant decrease (P<0.05) throughout the heat stress treatment period, except at the 40 min time point. Likewise, decreased αB-crystallin expression was also observed in the H9C2 cell line exposed to a high temperature in vitro, although its expression recovered to normal levels at later time points (80 and 100 min) and the cellular damage was less severe. The results suggest that αB-crystallin is mobilized early after exposure to a high temperature to interact with damaged proteins but that the myocardial cells cannot produce sufficient αB-crystallin for protection against heat stress. Lower αB-crystallin expression levels were accompanied by obvious cell/tissue damage, suggesting that the abundance of this protein is associated with protective effects in myocardial cells in vitro and in vivo. Thus, αB-crystallin is a potential biomarker of heat stress.Shu TangYingjun LvHongbo ChenAbdelnasir AdamYanfen ChengJörg HartungEndong BaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e86937 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shu Tang Yingjun Lv Hongbo Chen Abdelnasir Adam Yanfen Cheng Jörg Hartung Endong Bao Comparative analysis of αB-crystallin expression in heat-stressed myocardial cells in vivo and in vitro.
description	Relationships between αB-crystallin expression patterns and pathological changes of myocardial cells after heat stress were examined in vitro and in vivo in this study using the H9C2 cell line and Sprague-Dawley rats, respectively. Histopathological lesions, characterized by acute degeneration, karyopyknosis and loss of a defined nucleus, became more severe in rat hearts over the course of heat stress treatment from 20 min to 100 min. The expression of αB-crystallin in rat hearts showed a significant decrease (P<0.05) throughout the heat stress treatment period, except at the 40 min time point. Likewise, decreased αB-crystallin expression was also observed in the H9C2 cell line exposed to a high temperature in vitro, although its expression recovered to normal levels at later time points (80 and 100 min) and the cellular damage was less severe. The results suggest that αB-crystallin is mobilized early after exposure to a high temperature to interact with damaged proteins but that the myocardial cells cannot produce sufficient αB-crystallin for protection against heat stress. Lower αB-crystallin expression levels were accompanied by obvious cell/tissue damage, suggesting that the abundance of this protein is associated with protective effects in myocardial cells in vitro and in vivo. Thus, αB-crystallin is a potential biomarker of heat stress.
format	article
author	Shu Tang Yingjun Lv Hongbo Chen Abdelnasir Adam Yanfen Cheng Jörg Hartung Endong Bao
author_facet	Shu Tang Yingjun Lv Hongbo Chen Abdelnasir Adam Yanfen Cheng Jörg Hartung Endong Bao
author_sort	Shu Tang
title	Comparative analysis of αB-crystallin expression in heat-stressed myocardial cells in vivo and in vitro.
title_short	Comparative analysis of αB-crystallin expression in heat-stressed myocardial cells in vivo and in vitro.
title_full	Comparative analysis of αB-crystallin expression in heat-stressed myocardial cells in vivo and in vitro.
title_fullStr	Comparative analysis of αB-crystallin expression in heat-stressed myocardial cells in vivo and in vitro.
title_full_unstemmed	Comparative analysis of αB-crystallin expression in heat-stressed myocardial cells in vivo and in vitro.
title_sort	comparative analysis of αb-crystallin expression in heat-stressed myocardial cells in vivo and in vitro.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/4050d83b38d548f885988ae2f13af0fa
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Comparative analysis of αB-crystallin expression in heat-stressed myocardial cells in vivo and in vitro.

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