A Novel Pathogen Capturing Device for Removal and Detection

Abstract A simple technique that employs an antibody coated polydimethylsiloxane tube is used for effective capturing of bloodborne and foodborne pathogens. By recirculating the entire sample through the antibody coated tube, accumulation of target pathogens is achieved, thereby delivering a higher...

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Autores principales:	Gwangseong Kim, Horatiu Vinerean, Angelo Gaitas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/40568d5b04eb42ae800b586c855ce421
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spelling	oai:doaj.org-article:40568d5b04eb42ae800b586c855ce4212021-12-02T12:30:51ZA Novel Pathogen Capturing Device for Removal and Detection10.1038/s41598-017-05854-42045-2322https://doaj.org/article/40568d5b04eb42ae800b586c855ce4212017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05854-4https://doaj.org/toc/2045-2322Abstract A simple technique that employs an antibody coated polydimethylsiloxane tube is used for effective capturing of bloodborne and foodborne pathogens. By recirculating the entire sample through the antibody coated tube, accumulation of target pathogens is achieved, thereby delivering a higher concentration of pathogens in a small volume. The described method can provide an effective and economical solution to microbiology techniques that rely on enrichment, thereby expediting diagnostics. Using this method 80.3 ± 5.6% of Staphylococcus aureus with a starting concentration of ~107 CFU/mL and 95.4 ± 1.0% of Methicillin-resistant Staphylococcus aureus with starting concentration of ~104 CFU/mL were removed from 5 mL blood in a few hours. This concept was extended to live rats with an induced bloodstream S. aureus infection. A reduction of two orders of magnitude in the bacterial load of the rats was observed within a few hours. The same technique was used to capture a food pathogen, Salmonella typhimurium, with starting concentrations as low as ~100 CFU, from 100 or 250 mL of culture broth within similar timeframes as above. The feasibility for food pathogen testing applications was additionally confirmed by capturing and detecting S. typhimurium in ground chicken and ground beef.Gwangseong KimHoratiu VinereanAngelo GaitasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gwangseong Kim Horatiu Vinerean Angelo Gaitas A Novel Pathogen Capturing Device for Removal and Detection
description	Abstract A simple technique that employs an antibody coated polydimethylsiloxane tube is used for effective capturing of bloodborne and foodborne pathogens. By recirculating the entire sample through the antibody coated tube, accumulation of target pathogens is achieved, thereby delivering a higher concentration of pathogens in a small volume. The described method can provide an effective and economical solution to microbiology techniques that rely on enrichment, thereby expediting diagnostics. Using this method 80.3 ± 5.6% of Staphylococcus aureus with a starting concentration of ~107 CFU/mL and 95.4 ± 1.0% of Methicillin-resistant Staphylococcus aureus with starting concentration of ~104 CFU/mL were removed from 5 mL blood in a few hours. This concept was extended to live rats with an induced bloodstream S. aureus infection. A reduction of two orders of magnitude in the bacterial load of the rats was observed within a few hours. The same technique was used to capture a food pathogen, Salmonella typhimurium, with starting concentrations as low as ~100 CFU, from 100 or 250 mL of culture broth within similar timeframes as above. The feasibility for food pathogen testing applications was additionally confirmed by capturing and detecting S. typhimurium in ground chicken and ground beef.
format	article
author	Gwangseong Kim Horatiu Vinerean Angelo Gaitas
author_facet	Gwangseong Kim Horatiu Vinerean Angelo Gaitas
author_sort	Gwangseong Kim
title	A Novel Pathogen Capturing Device for Removal and Detection
title_short	A Novel Pathogen Capturing Device for Removal and Detection
title_full	A Novel Pathogen Capturing Device for Removal and Detection
title_fullStr	A Novel Pathogen Capturing Device for Removal and Detection
title_full_unstemmed	A Novel Pathogen Capturing Device for Removal and Detection
title_sort	novel pathogen capturing device for removal and detection
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/40568d5b04eb42ae800b586c855ce421
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A Novel Pathogen Capturing Device for Removal and Detection

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