Pangenome analysis reveals genetic isolation in Campylobacter hyointestinalis subspecies adapted to different mammalian hosts

Abstract Campylobacter hyointestinalis is an emerging pathogen currently divided in two subspecies: C. hyointestinalis subsp. lawsonii which is predominantly recovered from pigs, and C. hyointestinalis subsp. hyointestinalis which can be found in a much wider range of mammalian hosts. Despite C. hyo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Daniela Costa, Simon Lévesque, Nitin Kumar, Pablo Fresia, Ignacio Ferrés, Trevor D. Lawley, Gregorio Iraola
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4057af738fa64e8ab8ea643e411b70d7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4057af738fa64e8ab8ea643e411b70d7
record_format	dspace
spelling	oai:doaj.org-article:4057af738fa64e8ab8ea643e411b70d72021-12-02T12:14:50ZPangenome analysis reveals genetic isolation in Campylobacter hyointestinalis subspecies adapted to different mammalian hosts10.1038/s41598-021-82993-92045-2322https://doaj.org/article/4057af738fa64e8ab8ea643e411b70d72021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82993-9https://doaj.org/toc/2045-2322Abstract Campylobacter hyointestinalis is an emerging pathogen currently divided in two subspecies: C. hyointestinalis subsp. lawsonii which is predominantly recovered from pigs, and C. hyointestinalis subsp. hyointestinalis which can be found in a much wider range of mammalian hosts. Despite C. hyointestinalis being reported as an emerging pathogen, its evolutionary and host-associated diversification patterns are still vastly unexplored. For this reason, we generated whole-genome sequences of 13 C. hyointestinalis subsp. hyointestinalis strains and performed a comprehensive comparative analysis including publicly available C. hyointestinalis subsp. hyointestinalis and C. hyointestinalis subsp. lawsonii genomes, to gain insight into the genomic variation of these differentially-adapted subspecies. Both subspecies are distinct phylogenetic lineages which present an apparent barrier to homologous recombination, suggesting genetic isolation. This is further supported by accessory gene patterns that recapitulate the core genome phylogeny. Additionally, C. hyointestinalis subsp. hyointestinalis presents a bigger and more diverse accessory genome, which probably reflects its capacity to colonize different mammalian hosts unlike C. hyointestinalis subsp. lawsonii that is presumably host-restricted. This greater plasticity in the accessory genome of C. hyointestinalis subsp. hyointestinalis correlates to a higher incidence of genome-wide recombination events, that may be the underlying mechanism driving its diversification. Concordantly, both subspecies present distinct patterns of gene families involved in genome plasticity and DNA repair like CRISPR-associated proteins and restriction-modification systems. Together, our results provide an overview of the genetic mechanisms shaping the genomes of C. hyointestinalis subspecies, contributing to understand the biology of Campylobacter species that are increasingly recognized as emerging pathogens.Daniela CostaSimon LévesqueNitin KumarPablo FresiaIgnacio FerrésTrevor D. LawleyGregorio IraolaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniela Costa Simon Lévesque Nitin Kumar Pablo Fresia Ignacio Ferrés Trevor D. Lawley Gregorio Iraola Pangenome analysis reveals genetic isolation in Campylobacter hyointestinalis subspecies adapted to different mammalian hosts
description	Abstract Campylobacter hyointestinalis is an emerging pathogen currently divided in two subspecies: C. hyointestinalis subsp. lawsonii which is predominantly recovered from pigs, and C. hyointestinalis subsp. hyointestinalis which can be found in a much wider range of mammalian hosts. Despite C. hyointestinalis being reported as an emerging pathogen, its evolutionary and host-associated diversification patterns are still vastly unexplored. For this reason, we generated whole-genome sequences of 13 C. hyointestinalis subsp. hyointestinalis strains and performed a comprehensive comparative analysis including publicly available C. hyointestinalis subsp. hyointestinalis and C. hyointestinalis subsp. lawsonii genomes, to gain insight into the genomic variation of these differentially-adapted subspecies. Both subspecies are distinct phylogenetic lineages which present an apparent barrier to homologous recombination, suggesting genetic isolation. This is further supported by accessory gene patterns that recapitulate the core genome phylogeny. Additionally, C. hyointestinalis subsp. hyointestinalis presents a bigger and more diverse accessory genome, which probably reflects its capacity to colonize different mammalian hosts unlike C. hyointestinalis subsp. lawsonii that is presumably host-restricted. This greater plasticity in the accessory genome of C. hyointestinalis subsp. hyointestinalis correlates to a higher incidence of genome-wide recombination events, that may be the underlying mechanism driving its diversification. Concordantly, both subspecies present distinct patterns of gene families involved in genome plasticity and DNA repair like CRISPR-associated proteins and restriction-modification systems. Together, our results provide an overview of the genetic mechanisms shaping the genomes of C. hyointestinalis subspecies, contributing to understand the biology of Campylobacter species that are increasingly recognized as emerging pathogens.
format	article
author	Daniela Costa Simon Lévesque Nitin Kumar Pablo Fresia Ignacio Ferrés Trevor D. Lawley Gregorio Iraola
author_facet	Daniela Costa Simon Lévesque Nitin Kumar Pablo Fresia Ignacio Ferrés Trevor D. Lawley Gregorio Iraola
author_sort	Daniela Costa
title	Pangenome analysis reveals genetic isolation in Campylobacter hyointestinalis subspecies adapted to different mammalian hosts
title_short	Pangenome analysis reveals genetic isolation in Campylobacter hyointestinalis subspecies adapted to different mammalian hosts
title_full	Pangenome analysis reveals genetic isolation in Campylobacter hyointestinalis subspecies adapted to different mammalian hosts
title_fullStr	Pangenome analysis reveals genetic isolation in Campylobacter hyointestinalis subspecies adapted to different mammalian hosts
title_full_unstemmed	Pangenome analysis reveals genetic isolation in Campylobacter hyointestinalis subspecies adapted to different mammalian hosts
title_sort	pangenome analysis reveals genetic isolation in campylobacter hyointestinalis subspecies adapted to different mammalian hosts
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4057af738fa64e8ab8ea643e411b70d7
work_keys_str_mv	AT danielacosta pangenomeanalysisrevealsgeneticisolationincampylobacterhyointestinalissubspeciesadaptedtodifferentmammalianhosts AT simonlevesque pangenomeanalysisrevealsgeneticisolationincampylobacterhyointestinalissubspeciesadaptedtodifferentmammalianhosts AT nitinkumar pangenomeanalysisrevealsgeneticisolationincampylobacterhyointestinalissubspeciesadaptedtodifferentmammalianhosts AT pablofresia pangenomeanalysisrevealsgeneticisolationincampylobacterhyointestinalissubspeciesadaptedtodifferentmammalianhosts AT ignacioferres pangenomeanalysisrevealsgeneticisolationincampylobacterhyointestinalissubspeciesadaptedtodifferentmammalianhosts AT trevordlawley pangenomeanalysisrevealsgeneticisolationincampylobacterhyointestinalissubspeciesadaptedtodifferentmammalianhosts AT gregorioiraola pangenomeanalysisrevealsgeneticisolationincampylobacterhyointestinalissubspeciesadaptedtodifferentmammalianhosts
_version_	1718394568312881152

Pangenome analysis reveals genetic isolation in Campylobacter hyointestinalis subspecies adapted to different mammalian hosts

Ejemplares similares