Regulation of Transplanted Cell Homing by FGF1 and PDGFB after Doxorubicin Myocardial Injury

There is no effective treatment for the total recovery of myocardial injury caused by an anticancer drug, doxorubicin (Dox). In this study, using a Dox-induced cardiac injury model, we compared the cardioprotective effects of ventricular cells harvested from 11.5-day old embryonic mice (E11.5) with...

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Autores principales:	Mark Baguma-Nibasheka, Tiam Feridooni, Feixiong Zhang, Kishore B.S. Pasumarthi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ventricular cell migration growth factor and chemokine receptors doxorubicin cardiomyopathy cardiac dysfunction Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/405a832b3dc947d2acd71feb9324bc7b
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spelling	oai:doaj.org-article:405a832b3dc947d2acd71feb9324bc7b2021-11-25T17:10:15ZRegulation of Transplanted Cell Homing by FGF1 and PDGFB after Doxorubicin Myocardial Injury10.3390/cells101129982073-4409https://doaj.org/article/405a832b3dc947d2acd71feb9324bc7b2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/2998https://doaj.org/toc/2073-4409There is no effective treatment for the total recovery of myocardial injury caused by an anticancer drug, doxorubicin (Dox). In this study, using a Dox-induced cardiac injury model, we compared the cardioprotective effects of ventricular cells harvested from 11.5-day old embryonic mice (E11.5) with those from E14.5 embryos. Our results indicate that tail-vein-infused E11.5 ventricular cells are more efficient at homing into the injured adult myocardium, and are more angiogenic, than E14.5 ventricular cells. In addition, E11.5 cells were shown to mitigate the cardiomyopathic effects of Dox. In vitro, E11.5 ventricular cells were more migratory than E14.5 cells, and RT-qPCR analysis revealed that they express significantly higher levels of cytokine receptors <i>Fgfr1</i>, <i>Fgfr2</i>, <i>Pdgfra</i>, <i>Pdgfrb</i> and <i>Kit</i>. Remarkably, mRNA levels for <i>Fgf1, Fgf2, Pdgfa</i> and <i>Pdgfb</i> were also found to be elevated in the Dox-injured adult heart, as were the FGF1 and PDGFB protein levels. Addition of exogenous FGF1 or PDGFB was able to enhance E11.5 ventricular cell migration in vitro, and, whereas their neutralizing antibodies decreased cell migration. These results indicate that therapies raising the levels of FGF1 and PDGFB receptors in donor cells and or corresponding ligands in an injured heart could improve the efficacy of cell-based interventions for myocardial repair.Mark Baguma-NibashekaTiam FeridooniFeixiong ZhangKishore B.S. PasumarthiMDPI AGarticleventricular cell migrationgrowth factor and chemokine receptorsdoxorubicincardiomyopathycardiac dysfunctionBiology (General)QH301-705.5ENCells, Vol 10, Iss 2998, p 2998 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ventricular cell migration growth factor and chemokine receptors doxorubicin cardiomyopathy cardiac dysfunction Biology (General) QH301-705.5
spellingShingle	ventricular cell migration growth factor and chemokine receptors doxorubicin cardiomyopathy cardiac dysfunction Biology (General) QH301-705.5 Mark Baguma-Nibasheka Tiam Feridooni Feixiong Zhang Kishore B.S. Pasumarthi Regulation of Transplanted Cell Homing by FGF1 and PDGFB after Doxorubicin Myocardial Injury
description	There is no effective treatment for the total recovery of myocardial injury caused by an anticancer drug, doxorubicin (Dox). In this study, using a Dox-induced cardiac injury model, we compared the cardioprotective effects of ventricular cells harvested from 11.5-day old embryonic mice (E11.5) with those from E14.5 embryos. Our results indicate that tail-vein-infused E11.5 ventricular cells are more efficient at homing into the injured adult myocardium, and are more angiogenic, than E14.5 ventricular cells. In addition, E11.5 cells were shown to mitigate the cardiomyopathic effects of Dox. In vitro, E11.5 ventricular cells were more migratory than E14.5 cells, and RT-qPCR analysis revealed that they express significantly higher levels of cytokine receptors <i>Fgfr1</i>, <i>Fgfr2</i>, <i>Pdgfra</i>, <i>Pdgfrb</i> and <i>Kit</i>. Remarkably, mRNA levels for <i>Fgf1, Fgf2, Pdgfa</i> and <i>Pdgfb</i> were also found to be elevated in the Dox-injured adult heart, as were the FGF1 and PDGFB protein levels. Addition of exogenous FGF1 or PDGFB was able to enhance E11.5 ventricular cell migration in vitro, and, whereas their neutralizing antibodies decreased cell migration. These results indicate that therapies raising the levels of FGF1 and PDGFB receptors in donor cells and or corresponding ligands in an injured heart could improve the efficacy of cell-based interventions for myocardial repair.
format	article
author	Mark Baguma-Nibasheka Tiam Feridooni Feixiong Zhang Kishore B.S. Pasumarthi
author_facet	Mark Baguma-Nibasheka Tiam Feridooni Feixiong Zhang Kishore B.S. Pasumarthi
author_sort	Mark Baguma-Nibasheka
title	Regulation of Transplanted Cell Homing by FGF1 and PDGFB after Doxorubicin Myocardial Injury
title_short	Regulation of Transplanted Cell Homing by FGF1 and PDGFB after Doxorubicin Myocardial Injury
title_full	Regulation of Transplanted Cell Homing by FGF1 and PDGFB after Doxorubicin Myocardial Injury
title_fullStr	Regulation of Transplanted Cell Homing by FGF1 and PDGFB after Doxorubicin Myocardial Injury
title_full_unstemmed	Regulation of Transplanted Cell Homing by FGF1 and PDGFB after Doxorubicin Myocardial Injury
title_sort	regulation of transplanted cell homing by fgf1 and pdgfb after doxorubicin myocardial injury
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/405a832b3dc947d2acd71feb9324bc7b
work_keys_str_mv	AT markbagumanibasheka regulationoftransplantedcellhomingbyfgf1andpdgfbafterdoxorubicinmyocardialinjury AT tiamferidooni regulationoftransplantedcellhomingbyfgf1andpdgfbafterdoxorubicinmyocardialinjury AT feixiongzhang regulationoftransplantedcellhomingbyfgf1andpdgfbafterdoxorubicinmyocardialinjury AT kishorebspasumarthi regulationoftransplantedcellhomingbyfgf1andpdgfbafterdoxorubicinmyocardialinjury
_version_	1718412643125952512

Regulation of Transplanted Cell Homing by FGF1 and PDGFB after Doxorubicin Myocardial Injury

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