Evaluation of Immunogenicity and Efficacy of Fasciola hepatica Tetraspanin 2 (TSP2) Fused to E. coli Heat-Labile Enterotoxin B Subunit LTB Adjuvant Following Intranasal Vaccination of Cattle

Fasciolosis, caused by the liver flukes Fasciola hepatica and F. gigantica, is an economically important and globally distributed zoonotic disease. Liver fluke infections in livestock cause significant losses in production and are of particular concern to region...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Gemma Zerna, Vignesh A. Rathinasamy, Hayley Toet, Glenn Anderson, Robert Dempster, Terry W. Spithill, Travis Beddoe
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	vaccine <i>Fasciola hepatica</i> LTB AB<sub>5</sub> toxin tetraspanin tegument membrane Medicine R
Acceso en línea:	https://doaj.org/article/4069320d163d48e481e81c2abb2742e2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4069320d163d48e481e81c2abb2742e2
record_format	dspace
spelling	oai:doaj.org-article:4069320d163d48e481e81c2abb2742e22021-11-25T19:10:04ZEvaluation of Immunogenicity and Efficacy of <i>Fasciola hepatica</i> Tetraspanin 2 (TSP2) Fused to <i>E. coli</i> Heat-Labile Enterotoxin B Subunit LTB Adjuvant Following Intranasal Vaccination of Cattle10.3390/vaccines91112132076-393Xhttps://doaj.org/article/4069320d163d48e481e81c2abb2742e22021-10-01T00:00:00Zhttps://www.mdpi.com/2076-393X/9/11/1213https://doaj.org/toc/2076-393XFasciolosis, caused by the liver flukes <i>Fasciola hepatica</i> and <i>F. gigantica,</i> is an economically important and globally distributed zoonotic disease. Liver fluke infections in livestock cause significant losses in production and are of particular concern to regions where drug resistance is emerging. Antigens of the <i>F. hepatica</i> surface tegument represent promising vaccine candidates for controlling this disease. Tetraspanins are integral tegumental antigens that have shown partial protection as vaccine candidates against other trematode species. The <i>Escherichia coli</i> heat-labile enterotoxin’s B subunit (LTB) is a potent mucosal adjuvant capable of inducing an immune response to fused antigens. This study investigates the potential of <i>F. hepatica</i> tetraspanin 2 extracellular loop 2 (rFhTSP2) as a protective vaccine antigen and determines if fusion of FhTSP2 to LTB can enhance protection in cattle. Cattle were immunised subcutaneously with rFhTSP2 mixed in the Freund’s adjuvant and intranasally with rLTB-FhTSP2 in saline, accounting for equal molar ratios of tetraspanin in both groups. Vaccination with rFhTSP2 stimulated a strong specific serum IgG response, whereas there was no significant serum IgG response following rLTB-FhTSP2 intranasal vaccination. There was no substantial antigen specific serum IgA generated in all groups across the trial. Contrastingly, after the fluke challenge, a rise in antigen specific saliva IgA was observed in both vaccination groups on Day 42, with the rLTB-FhTSP2 vaccination group showing significant mucosal IgA production at Day 84. However, neither vaccine group showed a significant reduction of fluke burden nor faecal egg output. These results suggest that intranasal vaccination with rLTB-FhTSP2 does elicit a humoral mucosal response but further work is needed to evaluate if mucosal delivery of liver fluke antigens fused to LTB is a viable vaccine strategy.Gemma ZernaVignesh A. RathinasamyHayley ToetGlenn AndersonRobert DempsterTerry W. SpithillTravis BeddoeMDPI AGarticlevaccine<i>Fasciola hepatica</i>LTBAB<sub>5</sub> toxintetraspanintegument membraneMedicineRENVaccines, Vol 9, Iss 1213, p 1213 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	vaccine <i>Fasciola hepatica</i> LTB AB<sub>5</sub> toxin tetraspanin tegument membrane Medicine R
spellingShingle	vaccine <i>Fasciola hepatica</i> LTB AB<sub>5</sub> toxin tetraspanin tegument membrane Medicine R Gemma Zerna Vignesh A. Rathinasamy Hayley Toet Glenn Anderson Robert Dempster Terry W. Spithill Travis Beddoe Evaluation of Immunogenicity and Efficacy of <i>Fasciola hepatica</i> Tetraspanin 2 (TSP2) Fused to <i>E. coli</i> Heat-Labile Enterotoxin B Subunit LTB Adjuvant Following Intranasal Vaccination of Cattle
description	Fasciolosis, caused by the liver flukes <i>Fasciola hepatica</i> and <i>F. gigantica,</i> is an economically important and globally distributed zoonotic disease. Liver fluke infections in livestock cause significant losses in production and are of particular concern to regions where drug resistance is emerging. Antigens of the <i>F. hepatica</i> surface tegument represent promising vaccine candidates for controlling this disease. Tetraspanins are integral tegumental antigens that have shown partial protection as vaccine candidates against other trematode species. The <i>Escherichia coli</i> heat-labile enterotoxin’s B subunit (LTB) is a potent mucosal adjuvant capable of inducing an immune response to fused antigens. This study investigates the potential of <i>F. hepatica</i> tetraspanin 2 extracellular loop 2 (rFhTSP2) as a protective vaccine antigen and determines if fusion of FhTSP2 to LTB can enhance protection in cattle. Cattle were immunised subcutaneously with rFhTSP2 mixed in the Freund’s adjuvant and intranasally with rLTB-FhTSP2 in saline, accounting for equal molar ratios of tetraspanin in both groups. Vaccination with rFhTSP2 stimulated a strong specific serum IgG response, whereas there was no significant serum IgG response following rLTB-FhTSP2 intranasal vaccination. There was no substantial antigen specific serum IgA generated in all groups across the trial. Contrastingly, after the fluke challenge, a rise in antigen specific saliva IgA was observed in both vaccination groups on Day 42, with the rLTB-FhTSP2 vaccination group showing significant mucosal IgA production at Day 84. However, neither vaccine group showed a significant reduction of fluke burden nor faecal egg output. These results suggest that intranasal vaccination with rLTB-FhTSP2 does elicit a humoral mucosal response but further work is needed to evaluate if mucosal delivery of liver fluke antigens fused to LTB is a viable vaccine strategy.
format	article
author	Gemma Zerna Vignesh A. Rathinasamy Hayley Toet Glenn Anderson Robert Dempster Terry W. Spithill Travis Beddoe
author_facet	Gemma Zerna Vignesh A. Rathinasamy Hayley Toet Glenn Anderson Robert Dempster Terry W. Spithill Travis Beddoe
author_sort	Gemma Zerna
title	Evaluation of Immunogenicity and Efficacy of <i>Fasciola hepatica</i> Tetraspanin 2 (TSP2) Fused to <i>E. coli</i> Heat-Labile Enterotoxin B Subunit LTB Adjuvant Following Intranasal Vaccination of Cattle
title_short	Evaluation of Immunogenicity and Efficacy of <i>Fasciola hepatica</i> Tetraspanin 2 (TSP2) Fused to <i>E. coli</i> Heat-Labile Enterotoxin B Subunit LTB Adjuvant Following Intranasal Vaccination of Cattle
title_full	Evaluation of Immunogenicity and Efficacy of <i>Fasciola hepatica</i> Tetraspanin 2 (TSP2) Fused to <i>E. coli</i> Heat-Labile Enterotoxin B Subunit LTB Adjuvant Following Intranasal Vaccination of Cattle
title_fullStr	Evaluation of Immunogenicity and Efficacy of <i>Fasciola hepatica</i> Tetraspanin 2 (TSP2) Fused to <i>E. coli</i> Heat-Labile Enterotoxin B Subunit LTB Adjuvant Following Intranasal Vaccination of Cattle
title_full_unstemmed	Evaluation of Immunogenicity and Efficacy of <i>Fasciola hepatica</i> Tetraspanin 2 (TSP2) Fused to <i>E. coli</i> Heat-Labile Enterotoxin B Subunit LTB Adjuvant Following Intranasal Vaccination of Cattle
title_sort	evaluation of immunogenicity and efficacy of <i>fasciola hepatica</i> tetraspanin 2 (tsp2) fused to <i>e. coli</i> heat-labile enterotoxin b subunit ltb adjuvant following intranasal vaccination of cattle
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4069320d163d48e481e81c2abb2742e2
work_keys_str_mv	AT gemmazerna evaluationofimmunogenicityandefficacyofifasciolahepaticaitetraspanin2tsp2fusedtoiecoliiheatlabileenterotoxinbsubunitltbadjuvantfollowingintranasalvaccinationofcattle AT vignesharathinasamy evaluationofimmunogenicityandefficacyofifasciolahepaticaitetraspanin2tsp2fusedtoiecoliiheatlabileenterotoxinbsubunitltbadjuvantfollowingintranasalvaccinationofcattle AT hayleytoet evaluationofimmunogenicityandefficacyofifasciolahepaticaitetraspanin2tsp2fusedtoiecoliiheatlabileenterotoxinbsubunitltbadjuvantfollowingintranasalvaccinationofcattle AT glennanderson evaluationofimmunogenicityandefficacyofifasciolahepaticaitetraspanin2tsp2fusedtoiecoliiheatlabileenterotoxinbsubunitltbadjuvantfollowingintranasalvaccinationofcattle AT robertdempster evaluationofimmunogenicityandefficacyofifasciolahepaticaitetraspanin2tsp2fusedtoiecoliiheatlabileenterotoxinbsubunitltbadjuvantfollowingintranasalvaccinationofcattle AT terrywspithill evaluationofimmunogenicityandefficacyofifasciolahepaticaitetraspanin2tsp2fusedtoiecoliiheatlabileenterotoxinbsubunitltbadjuvantfollowingintranasalvaccinationofcattle AT travisbeddoe evaluationofimmunogenicityandefficacyofifasciolahepaticaitetraspanin2tsp2fusedtoiecoliiheatlabileenterotoxinbsubunitltbadjuvantfollowingintranasalvaccinationofcattle
_version_	1718410211120644096

Evaluation of Immunogenicity and Efficacy of <i>Fasciola hepatica</i> Tetraspanin 2 (TSP2) Fused to <i>E. coli</i> Heat-Labile Enterotoxin B Subunit LTB Adjuvant Following Intranasal Vaccination of Cattle

Ejemplares similares