Ti-GO-Ag nanocomposite: the effect of content level on the antimicrobial activity and cytotoxicity

Jianfeng Jin,1,2 Li Zhang,3 Mengqi Shi,3 Yumei Zhang,3 Qintao Wang11State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Periodontology, School of Stom...

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Autores principales:	Jin J, Zhang L, Shi M, Zhang Y, Wang Q
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	GO-Ag-Ti multiphase nanocomposite Content level Antibacterial activity Bactericidal mechanism Cytotoxicity Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/406ba0b2afd44d40bebb3a53b8f33948
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spelling	oai:doaj.org-article:406ba0b2afd44d40bebb3a53b8f339482021-12-02T03:54:12ZTi-GO-Ag nanocomposite: the effect of content level on the antimicrobial activity and cytotoxicity1178-2013https://doaj.org/article/406ba0b2afd44d40bebb3a53b8f339482017-06-01T00:00:00Zhttps://www.dovepress.com/ti-go-ag-nanocomposite-the-effect-of-content-level-on-the-antimicrobia-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jianfeng Jin,1,2 Li Zhang,3 Mengqi Shi,3 Yumei Zhang,3 Qintao Wang11State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi’an, 2Department of General Dentistry, Kunming Municipal Stomatology Hospital, Kunming, 3State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an, People’s Republic of China Abstract: Surface modification of titanium (Ti) implants are extensively studied in order to obtain prominent biocompatibility and antimicrobial activity, especially preventing implant-associated infection. In this study, Ti substrates surface were modified by graphene oxide (GO) thin film and silver (Ag) nanoparticles via electroplating and ultraviolet reduction methods so as to achieve this purpose. Microstructures, distribution, quantities and spectral peaks of GO and Ag loading on the Ti sheets surface were characterized. GO-Ag-Ti multiphase nanocomposite exhibited excellent antimicrobial ability and anti-adherence performance. Subsequently, morphology, membrane integrity, apoptosis and relative genes expression of bacteria incubated on the Ti samples surface were monitored to reveal the bactericidal mechanism. Additionally, the cytotoxicity of Ti substrates incorporating GO thin film and Ag nanoparticles were investigated. GO-Ag-Ti composite configuration that have outstanding antibacterial properties will provide the foundation to study bone integration in vitro and in vivo in the future.Keywords: GO-Ag-Ti multiphase nanocomposite, microstructure and quantities, antibacterial activity, bactericidal mechanism, cytotoxicityJin JZhang LShi MZhang YWang QDove Medical PressarticleGO-Ag-Ti multiphase nanocompositeContent levelAntibacterial activityBactericidal mechanismCytotoxicityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 4209-4224 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	GO-Ag-Ti multiphase nanocomposite Content level Antibacterial activity Bactericidal mechanism Cytotoxicity Medicine (General) R5-920
spellingShingle	GO-Ag-Ti multiphase nanocomposite Content level Antibacterial activity Bactericidal mechanism Cytotoxicity Medicine (General) R5-920 Jin J Zhang L Shi M Zhang Y Wang Q Ti-GO-Ag nanocomposite: the effect of content level on the antimicrobial activity and cytotoxicity
description	Jianfeng Jin,1,2 Li Zhang,3 Mengqi Shi,3 Yumei Zhang,3 Qintao Wang11State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi’an, 2Department of General Dentistry, Kunming Municipal Stomatology Hospital, Kunming, 3State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an, People’s Republic of China Abstract: Surface modification of titanium (Ti) implants are extensively studied in order to obtain prominent biocompatibility and antimicrobial activity, especially preventing implant-associated infection. In this study, Ti substrates surface were modified by graphene oxide (GO) thin film and silver (Ag) nanoparticles via electroplating and ultraviolet reduction methods so as to achieve this purpose. Microstructures, distribution, quantities and spectral peaks of GO and Ag loading on the Ti sheets surface were characterized. GO-Ag-Ti multiphase nanocomposite exhibited excellent antimicrobial ability and anti-adherence performance. Subsequently, morphology, membrane integrity, apoptosis and relative genes expression of bacteria incubated on the Ti samples surface were monitored to reveal the bactericidal mechanism. Additionally, the cytotoxicity of Ti substrates incorporating GO thin film and Ag nanoparticles were investigated. GO-Ag-Ti composite configuration that have outstanding antibacterial properties will provide the foundation to study bone integration in vitro and in vivo in the future.Keywords: GO-Ag-Ti multiphase nanocomposite, microstructure and quantities, antibacterial activity, bactericidal mechanism, cytotoxicity
format	article
author	Jin J Zhang L Shi M Zhang Y Wang Q
author_facet	Jin J Zhang L Shi M Zhang Y Wang Q
author_sort	Jin J
title	Ti-GO-Ag nanocomposite: the effect of content level on the antimicrobial activity and cytotoxicity
title_short	Ti-GO-Ag nanocomposite: the effect of content level on the antimicrobial activity and cytotoxicity
title_full	Ti-GO-Ag nanocomposite: the effect of content level on the antimicrobial activity and cytotoxicity
title_fullStr	Ti-GO-Ag nanocomposite: the effect of content level on the antimicrobial activity and cytotoxicity
title_full_unstemmed	Ti-GO-Ag nanocomposite: the effect of content level on the antimicrobial activity and cytotoxicity
title_sort	ti-go-ag nanocomposite: the effect of content level on the antimicrobial activity and cytotoxicity
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/406ba0b2afd44d40bebb3a53b8f33948
work_keys_str_mv	AT jinj tigoagnanocompositetheeffectofcontentlevelontheantimicrobialactivityandcytotoxicity AT zhangl tigoagnanocompositetheeffectofcontentlevelontheantimicrobialactivityandcytotoxicity AT shim tigoagnanocompositetheeffectofcontentlevelontheantimicrobialactivityandcytotoxicity AT zhangy tigoagnanocompositetheeffectofcontentlevelontheantimicrobialactivityandcytotoxicity AT wangq tigoagnanocompositetheeffectofcontentlevelontheantimicrobialactivityandcytotoxicity
_version_	1718401544202747904

Ti-GO-Ag nanocomposite: the effect of content level on the antimicrobial activity and cytotoxicity

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