Effect of Hydrothermal Media on the in-situ Whisker Growth on Biphasic Calcium Phosphate Ceramics

Cong Feng, Yonghao Wu, Quanle Cao, Xiangfeng Li, Xiangdong Zhu, Xingdong Zhang National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan, People’s Republic of ChinaCorrespondence: Xiangfeng Li; Xiangdong ZhuSichuan University, No. 24 South Section 1, Yihua...

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Autores principales:	Feng C, Wu Y, Cao Q, Li X, Zhu X, Zhang X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	in-situ whisker hydrothermal treatment biphasic calcium phosphate mechanical property bioactivity Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/406c622be8a24328a0ee3764132f2bd3
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spelling	oai:doaj.org-article:406c622be8a24328a0ee3764132f2bd32021-12-02T13:35:41ZEffect of Hydrothermal Media on the in-situ Whisker Growth on Biphasic Calcium Phosphate Ceramics1178-2013https://doaj.org/article/406c622be8a24328a0ee3764132f2bd32021-01-01T00:00:00Zhttps://www.dovepress.com/effect-of-hydrothermal-media-on-the-in-situ-whisker-growth-on-biphasic-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Cong Feng, Yonghao Wu, Quanle Cao, Xiangfeng Li, Xiangdong Zhu, Xingdong Zhang National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan, People’s Republic of ChinaCorrespondence: Xiangfeng Li; Xiangdong ZhuSichuan University, No. 24 South Section 1, Yihuan Road, Chengdu 610065, People’s Republic of ChinaTel/Fax +86-2885417654Email hkdlxiangfeng@163.com; zhu_xd1973@scu.edu.cnBackground: There is still a big challenge to achieve a balance between mechanical characteristics and biological properties in biphasic calcium phosphate (BCP) ceramics.Purpose: The present study focused on the in-situ whisker growth on BCP ceramics via different hydrothermal treatments and investigated the influences of these whiskers on the mechanical property and biological performance of the ceramics.Methods: Five kinds of BCP ceramics with in-situ whisker growth, ie, BCP-C, BCP-HNO3, BCP-Citric, BCP-NaOH, BCP-CaCl2 and BCP-Na3PO4 were fabricated by different hydrothermal treatments. The phase compositions, morphologies, crystal structures and mechanical strengths of the obtained BCP ceramics were firstly characterized. Then, the in vitro cell adhesion, proliferation and alkaline  phosphatase (ALP) activity of bone marrow stromal cells (BMSCs) on the BCP ceramics were evaluated. Lastly, the effects of in-situ whisker growth on the bone-like apatite formation abilities of BCP ceramics were also investigated by immersing them in simulated body fluid (SBF).Results: The results demonstrated that the hydrothermal conditions, especially the hydrothermal media, were crucial to determine the phase composition and morphology of the in-situ whisker. Especially among the five media used (HNO3, Citric, NaOH, CaCl2 and Na3PO4), the Na3PO4 treatment resulted in the shortest whisker with a unique hollow structure, and kept the original biphasic composition. All five kinds of whiskers increased the mechanical strength of BCP ceramics to some extent, and showed the good ability of bone-like apatite formation. The in vitro cell study demonstrated that the in-situ whisker growth had no adverse but even positive effect on the adhesion, proliferation and ALP activity of BMSCs.Conclusion: Due to the growth of in-situ whiskers, the mechanical property and biological performance of the obtained BCP ceramics could increase simultaneously. Therefore, in-situ whiskers growth offers a promising strategy for the expanded application of BCP ceramics to meet the requirements of regenerative medicine.Keywords: in-situ whisker, hydrothermal treatment, biphasic calcium phosphate, mechanical property, bioactivityFeng CWu YCao QLi XZhu XZhang XDove Medical Pressarticlein-situ whiskerhydrothermal treatmentbiphasic calcium phosphatemechanical propertybioactivityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 147-159 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	in-situ whisker hydrothermal treatment biphasic calcium phosphate mechanical property bioactivity Medicine (General) R5-920
spellingShingle	in-situ whisker hydrothermal treatment biphasic calcium phosphate mechanical property bioactivity Medicine (General) R5-920 Feng C Wu Y Cao Q Li X Zhu X Zhang X Effect of Hydrothermal Media on the in-situ Whisker Growth on Biphasic Calcium Phosphate Ceramics
description	Cong Feng, Yonghao Wu, Quanle Cao, Xiangfeng Li, Xiangdong Zhu, Xingdong Zhang National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan, People’s Republic of ChinaCorrespondence: Xiangfeng Li; Xiangdong ZhuSichuan University, No. 24 South Section 1, Yihuan Road, Chengdu 610065, People’s Republic of ChinaTel/Fax +86-2885417654Email hkdlxiangfeng@163.com; zhu_xd1973@scu.edu.cnBackground: There is still a big challenge to achieve a balance between mechanical characteristics and biological properties in biphasic calcium phosphate (BCP) ceramics.Purpose: The present study focused on the in-situ whisker growth on BCP ceramics via different hydrothermal treatments and investigated the influences of these whiskers on the mechanical property and biological performance of the ceramics.Methods: Five kinds of BCP ceramics with in-situ whisker growth, ie, BCP-C, BCP-HNO3, BCP-Citric, BCP-NaOH, BCP-CaCl2 and BCP-Na3PO4 were fabricated by different hydrothermal treatments. The phase compositions, morphologies, crystal structures and mechanical strengths of the obtained BCP ceramics were firstly characterized. Then, the in vitro cell adhesion, proliferation and alkaline  phosphatase (ALP) activity of bone marrow stromal cells (BMSCs) on the BCP ceramics were evaluated. Lastly, the effects of in-situ whisker growth on the bone-like apatite formation abilities of BCP ceramics were also investigated by immersing them in simulated body fluid (SBF).Results: The results demonstrated that the hydrothermal conditions, especially the hydrothermal media, were crucial to determine the phase composition and morphology of the in-situ whisker. Especially among the five media used (HNO3, Citric, NaOH, CaCl2 and Na3PO4), the Na3PO4 treatment resulted in the shortest whisker with a unique hollow structure, and kept the original biphasic composition. All five kinds of whiskers increased the mechanical strength of BCP ceramics to some extent, and showed the good ability of bone-like apatite formation. The in vitro cell study demonstrated that the in-situ whisker growth had no adverse but even positive effect on the adhesion, proliferation and ALP activity of BMSCs.Conclusion: Due to the growth of in-situ whiskers, the mechanical property and biological performance of the obtained BCP ceramics could increase simultaneously. Therefore, in-situ whiskers growth offers a promising strategy for the expanded application of BCP ceramics to meet the requirements of regenerative medicine.Keywords: in-situ whisker, hydrothermal treatment, biphasic calcium phosphate, mechanical property, bioactivity
format	article
author	Feng C Wu Y Cao Q Li X Zhu X Zhang X
author_facet	Feng C Wu Y Cao Q Li X Zhu X Zhang X
author_sort	Feng C
title	Effect of Hydrothermal Media on the in-situ Whisker Growth on Biphasic Calcium Phosphate Ceramics
title_short	Effect of Hydrothermal Media on the in-situ Whisker Growth on Biphasic Calcium Phosphate Ceramics
title_full	Effect of Hydrothermal Media on the in-situ Whisker Growth on Biphasic Calcium Phosphate Ceramics
title_fullStr	Effect of Hydrothermal Media on the in-situ Whisker Growth on Biphasic Calcium Phosphate Ceramics
title_full_unstemmed	Effect of Hydrothermal Media on the in-situ Whisker Growth on Biphasic Calcium Phosphate Ceramics
title_sort	effect of hydrothermal media on the in-situ whisker growth on biphasic calcium phosphate ceramics
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/406c622be8a24328a0ee3764132f2bd3
work_keys_str_mv	AT fengc effectofhydrothermalmediaontheinsituwhiskergrowthonbiphasiccalciumphosphateceramics AT wuy effectofhydrothermalmediaontheinsituwhiskergrowthonbiphasiccalciumphosphateceramics AT caoq effectofhydrothermalmediaontheinsituwhiskergrowthonbiphasiccalciumphosphateceramics AT lix effectofhydrothermalmediaontheinsituwhiskergrowthonbiphasiccalciumphosphateceramics AT zhux effectofhydrothermalmediaontheinsituwhiskergrowthonbiphasiccalciumphosphateceramics AT zhangx effectofhydrothermalmediaontheinsituwhiskergrowthonbiphasiccalciumphosphateceramics
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Effect of Hydrothermal Media on the in-situ Whisker Growth on Biphasic Calcium Phosphate Ceramics

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