First Immunoassay for Measuring Isoaspartate in Human Serum Albumin

Isoaspartate (isoAsp) is a damaging amino acid residue formed in proteins mostly as a result of spontaneous deamidation of asparaginyl residues. An association has been found between isoAsp in human serum albumin (HSA) and Alzheimer’s disease (AD). Here we report on a novel monoclonal antibody (mAb)...

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Autores principales:	Jijing Wang, Susanna L. Lundström, Sven Seelow, Sergey Rodin, Zhaowei Meng, Juan Astorga-Wells, Qinyu Jia, Roman A. Zubarev
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	in vitro diagnostics blood analysis monoclonal antibody (mAb) mass spectrometry enzyme-linked immunosorbent assay (ELISA) human serum albumin (HSA) Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/406db28c57ae42aa81e660866e97c59f
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spelling	oai:doaj.org-article:406db28c57ae42aa81e660866e97c59f2021-11-11T18:39:15ZFirst Immunoassay for Measuring Isoaspartate in Human Serum Albumin10.3390/molecules262167091420-3049https://doaj.org/article/406db28c57ae42aa81e660866e97c59f2021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6709https://doaj.org/toc/1420-3049Isoaspartate (isoAsp) is a damaging amino acid residue formed in proteins mostly as a result of spontaneous deamidation of asparaginyl residues. An association has been found between isoAsp in human serum albumin (HSA) and Alzheimer’s disease (AD). Here we report on a novel monoclonal antibody (mAb) 1A3 with excellent specificity to isoAsp in the functionally important domain of HSA. Based on 1A3 mAb, an indirect enzyme-linked immunosorbent assay (ELISA) was developed, and the isoAsp occupancy in 100 healthy plasma samples was quantified for the first time, providing the average value of (0.74 ± 0.13)%. These results suggest potential of isoAsp measurements for supplementary AD diagnostics as well as for assessing the freshness of stored donor blood and its suitability for transfusion.Jijing WangSusanna L. LundströmSven SeelowSergey RodinZhaowei MengJuan Astorga-WellsQinyu JiaRoman A. ZubarevMDPI AGarticlein vitro diagnosticsblood analysismonoclonal antibody (mAb)mass spectrometryenzyme-linked immunosorbent assay (ELISA)human serum albumin (HSA)Organic chemistryQD241-441ENMolecules, Vol 26, Iss 6709, p 6709 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	in vitro diagnostics blood analysis monoclonal antibody (mAb) mass spectrometry enzyme-linked immunosorbent assay (ELISA) human serum albumin (HSA) Organic chemistry QD241-441
spellingShingle	in vitro diagnostics blood analysis monoclonal antibody (mAb) mass spectrometry enzyme-linked immunosorbent assay (ELISA) human serum albumin (HSA) Organic chemistry QD241-441 Jijing Wang Susanna L. Lundström Sven Seelow Sergey Rodin Zhaowei Meng Juan Astorga-Wells Qinyu Jia Roman A. Zubarev First Immunoassay for Measuring Isoaspartate in Human Serum Albumin
description	Isoaspartate (isoAsp) is a damaging amino acid residue formed in proteins mostly as a result of spontaneous deamidation of asparaginyl residues. An association has been found between isoAsp in human serum albumin (HSA) and Alzheimer’s disease (AD). Here we report on a novel monoclonal antibody (mAb) 1A3 with excellent specificity to isoAsp in the functionally important domain of HSA. Based on 1A3 mAb, an indirect enzyme-linked immunosorbent assay (ELISA) was developed, and the isoAsp occupancy in 100 healthy plasma samples was quantified for the first time, providing the average value of (0.74 ± 0.13)%. These results suggest potential of isoAsp measurements for supplementary AD diagnostics as well as for assessing the freshness of stored donor blood and its suitability for transfusion.
format	article
author	Jijing Wang Susanna L. Lundström Sven Seelow Sergey Rodin Zhaowei Meng Juan Astorga-Wells Qinyu Jia Roman A. Zubarev
author_facet	Jijing Wang Susanna L. Lundström Sven Seelow Sergey Rodin Zhaowei Meng Juan Astorga-Wells Qinyu Jia Roman A. Zubarev
author_sort	Jijing Wang
title	First Immunoassay for Measuring Isoaspartate in Human Serum Albumin
title_short	First Immunoassay for Measuring Isoaspartate in Human Serum Albumin
title_full	First Immunoassay for Measuring Isoaspartate in Human Serum Albumin
title_fullStr	First Immunoassay for Measuring Isoaspartate in Human Serum Albumin
title_full_unstemmed	First Immunoassay for Measuring Isoaspartate in Human Serum Albumin
title_sort	first immunoassay for measuring isoaspartate in human serum albumin
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/406db28c57ae42aa81e660866e97c59f
work_keys_str_mv	AT jijingwang firstimmunoassayformeasuringisoaspartateinhumanserumalbumin AT susannallundstrom firstimmunoassayformeasuringisoaspartateinhumanserumalbumin AT svenseelow firstimmunoassayformeasuringisoaspartateinhumanserumalbumin AT sergeyrodin firstimmunoassayformeasuringisoaspartateinhumanserumalbumin AT zhaoweimeng firstimmunoassayformeasuringisoaspartateinhumanserumalbumin AT juanastorgawells firstimmunoassayformeasuringisoaspartateinhumanserumalbumin AT qinyujia firstimmunoassayformeasuringisoaspartateinhumanserumalbumin AT romanazubarev firstimmunoassayformeasuringisoaspartateinhumanserumalbumin
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First Immunoassay for Measuring Isoaspartate in Human Serum Albumin

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