The effect of a DNA repair gene on cellular invasiveness: XRCC3 over-expression in breast cancer cells.

Over-expression of DNA repair genes has been associated with resistance to radiation and DNA-damage induced by chemotherapeutic agents such as cisplatin. More recently, based on the analysis of genome expression profiling, it was proposed that over-expression of DNA repair genes enhances the invasiv...

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Autores principales:	Veronica L Martinez-Marignac, Amélie Rodrigue, David Davidson, Martin Couillard, Ala-Eddin Al-Moustafa, Mark Abramovitz, William D Foulkes, Jean-Yves Masson, Raquel Aloyz
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/406f1e6780a842c497eab8d0eae127e7
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spelling	oai:doaj.org-article:406f1e6780a842c497eab8d0eae127e72021-11-18T06:59:59ZThe effect of a DNA repair gene on cellular invasiveness: XRCC3 over-expression in breast cancer cells.1932-620310.1371/journal.pone.0016394https://doaj.org/article/406f1e6780a842c497eab8d0eae127e72011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21283680/?tool=EBIhttps://doaj.org/toc/1932-6203Over-expression of DNA repair genes has been associated with resistance to radiation and DNA-damage induced by chemotherapeutic agents such as cisplatin. More recently, based on the analysis of genome expression profiling, it was proposed that over-expression of DNA repair genes enhances the invasive behaviour of tumour cells. In this study we present experimental evidence utilizing functional assays to test this hypothesis. We assessed the effect of the DNA repair proteins known as X-ray complementing protein 3 (XRCC3) and RAD51, to the invasive behavior of the MCF-7 luminal epithelial-like and BT20 basal-like triple negative human breast cancer cell lines. We report that stable or transient over-expression of XRCC3 but not RAD51 increased invasiveness in both cell lines in vitro. Moreover, XRCC3 over-expressing MCF-7 cells also showed a higher tumorigenesis in vivo and this phenotype was associated with increased activity of the metalloproteinase MMP-9 and the expression of known modulators of cell-cell adhesion and metastasis such as CD44, ID-1, DDR1 and TFF1. Our results suggest that in addition to its' role in facilitating repair of DNA damage, XRCC3 affects invasiveness of breast cancer cell lines and the expression of genes associated with cell adhesion and invasion.Veronica L Martinez-MarignacAmélie RodrigueDavid DavidsonMartin CouillardAla-Eddin Al-MoustafaMark AbramovitzWilliam D FoulkesJean-Yves MassonRaquel AloyzPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e16394 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Veronica L Martinez-Marignac Amélie Rodrigue David Davidson Martin Couillard Ala-Eddin Al-Moustafa Mark Abramovitz William D Foulkes Jean-Yves Masson Raquel Aloyz The effect of a DNA repair gene on cellular invasiveness: XRCC3 over-expression in breast cancer cells.
description	Over-expression of DNA repair genes has been associated with resistance to radiation and DNA-damage induced by chemotherapeutic agents such as cisplatin. More recently, based on the analysis of genome expression profiling, it was proposed that over-expression of DNA repair genes enhances the invasive behaviour of tumour cells. In this study we present experimental evidence utilizing functional assays to test this hypothesis. We assessed the effect of the DNA repair proteins known as X-ray complementing protein 3 (XRCC3) and RAD51, to the invasive behavior of the MCF-7 luminal epithelial-like and BT20 basal-like triple negative human breast cancer cell lines. We report that stable or transient over-expression of XRCC3 but not RAD51 increased invasiveness in both cell lines in vitro. Moreover, XRCC3 over-expressing MCF-7 cells also showed a higher tumorigenesis in vivo and this phenotype was associated with increased activity of the metalloproteinase MMP-9 and the expression of known modulators of cell-cell adhesion and metastasis such as CD44, ID-1, DDR1 and TFF1. Our results suggest that in addition to its' role in facilitating repair of DNA damage, XRCC3 affects invasiveness of breast cancer cell lines and the expression of genes associated with cell adhesion and invasion.
format	article
author	Veronica L Martinez-Marignac Amélie Rodrigue David Davidson Martin Couillard Ala-Eddin Al-Moustafa Mark Abramovitz William D Foulkes Jean-Yves Masson Raquel Aloyz
author_facet	Veronica L Martinez-Marignac Amélie Rodrigue David Davidson Martin Couillard Ala-Eddin Al-Moustafa Mark Abramovitz William D Foulkes Jean-Yves Masson Raquel Aloyz
author_sort	Veronica L Martinez-Marignac
title	The effect of a DNA repair gene on cellular invasiveness: XRCC3 over-expression in breast cancer cells.
title_short	The effect of a DNA repair gene on cellular invasiveness: XRCC3 over-expression in breast cancer cells.
title_full	The effect of a DNA repair gene on cellular invasiveness: XRCC3 over-expression in breast cancer cells.
title_fullStr	The effect of a DNA repair gene on cellular invasiveness: XRCC3 over-expression in breast cancer cells.
title_full_unstemmed	The effect of a DNA repair gene on cellular invasiveness: XRCC3 over-expression in breast cancer cells.
title_sort	effect of a dna repair gene on cellular invasiveness: xrcc3 over-expression in breast cancer cells.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/406f1e6780a842c497eab8d0eae127e7
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The effect of a DNA repair gene on cellular invasiveness: XRCC3 over-expression in breast cancer cells.

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