Embryonic echocardiography for assessment of congenital and functional cardiac defects

Summary: Cardiac function and morphology by mouse fetal echocardiography can be assessed by scanning the uterus extracted from the abdominal cavity (trans-uterine ultrasound) or the womb (trans-abdominal ultrasound). Advantages of trans-abdominal ultrasound include (1) non-invasive longitudinal anal...

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Autores principales: Ivan Menendez-Montes, Maria Villalba-Orero, Beatriz Escobar, Silvia Martin-Puig
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Lenguaje:EN
Publicado: Elsevier 2021
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Acceso en línea:https://doaj.org/article/406f90a7994f4b2f87388009d7e6dcd2
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spelling oai:doaj.org-article:406f90a7994f4b2f87388009d7e6dcd22021-12-04T04:35:58ZEmbryonic echocardiography for assessment of congenital and functional cardiac defects2666-166710.1016/j.xpro.2021.100980https://doaj.org/article/406f90a7994f4b2f87388009d7e6dcd22021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006869https://doaj.org/toc/2666-1667Summary: Cardiac function and morphology by mouse fetal echocardiography can be assessed by scanning the uterus extracted from the abdominal cavity (trans-uterine ultrasound) or the womb (trans-abdominal ultrasound). Advantages of trans-abdominal ultrasound include (1) non-invasive longitudinal analysis at different stages, reducing animal use; and (2) maintenance of natural environment, diminishing perturbations on functional parameters, which are more frequent in trans-uterine conditions. Here we describe both approaches, explaining how to identify congenital cardiac defects and defining the correlation between echocardiography findings and histological analysis.For complete details on the use and execution of this protocol, please refer to (Menendez-Montes et al., 2016) and (Menendez-Montes et al., 2021).Ivan Menendez-MontesMaria Villalba-OreroBeatriz EscobarSilvia Martin-PuigElsevierarticleDevelopmental biologyHealth SciencesModel OrganismsScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100980- (2021)
institution DOAJ
collection DOAJ
language EN
topic Developmental biology
Health Sciences
Model Organisms
Science (General)
Q1-390
spellingShingle Developmental biology
Health Sciences
Model Organisms
Science (General)
Q1-390
Ivan Menendez-Montes
Maria Villalba-Orero
Beatriz Escobar
Silvia Martin-Puig
Embryonic echocardiography for assessment of congenital and functional cardiac defects
description Summary: Cardiac function and morphology by mouse fetal echocardiography can be assessed by scanning the uterus extracted from the abdominal cavity (trans-uterine ultrasound) or the womb (trans-abdominal ultrasound). Advantages of trans-abdominal ultrasound include (1) non-invasive longitudinal analysis at different stages, reducing animal use; and (2) maintenance of natural environment, diminishing perturbations on functional parameters, which are more frequent in trans-uterine conditions. Here we describe both approaches, explaining how to identify congenital cardiac defects and defining the correlation between echocardiography findings and histological analysis.For complete details on the use and execution of this protocol, please refer to (Menendez-Montes et al., 2016) and (Menendez-Montes et al., 2021).
format article
author Ivan Menendez-Montes
Maria Villalba-Orero
Beatriz Escobar
Silvia Martin-Puig
author_facet Ivan Menendez-Montes
Maria Villalba-Orero
Beatriz Escobar
Silvia Martin-Puig
author_sort Ivan Menendez-Montes
title Embryonic echocardiography for assessment of congenital and functional cardiac defects
title_short Embryonic echocardiography for assessment of congenital and functional cardiac defects
title_full Embryonic echocardiography for assessment of congenital and functional cardiac defects
title_fullStr Embryonic echocardiography for assessment of congenital and functional cardiac defects
title_full_unstemmed Embryonic echocardiography for assessment of congenital and functional cardiac defects
title_sort embryonic echocardiography for assessment of congenital and functional cardiac defects
publisher Elsevier
publishDate 2021
url https://doaj.org/article/406f90a7994f4b2f87388009d7e6dcd2
work_keys_str_mv AT ivanmenendezmontes embryonicechocardiographyforassessmentofcongenitalandfunctionalcardiacdefects
AT mariavillalbaorero embryonicechocardiographyforassessmentofcongenitalandfunctionalcardiacdefects
AT beatrizescobar embryonicechocardiographyforassessmentofcongenitalandfunctionalcardiacdefects
AT silviamartinpuig embryonicechocardiographyforassessmentofcongenitalandfunctionalcardiacdefects
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