Embryonic echocardiography for assessment of congenital and functional cardiac defects
Summary: Cardiac function and morphology by mouse fetal echocardiography can be assessed by scanning the uterus extracted from the abdominal cavity (trans-uterine ultrasound) or the womb (trans-abdominal ultrasound). Advantages of trans-abdominal ultrasound include (1) non-invasive longitudinal anal...
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2021
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oai:doaj.org-article:406f90a7994f4b2f87388009d7e6dcd22021-12-04T04:35:58ZEmbryonic echocardiography for assessment of congenital and functional cardiac defects2666-166710.1016/j.xpro.2021.100980https://doaj.org/article/406f90a7994f4b2f87388009d7e6dcd22021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006869https://doaj.org/toc/2666-1667Summary: Cardiac function and morphology by mouse fetal echocardiography can be assessed by scanning the uterus extracted from the abdominal cavity (trans-uterine ultrasound) or the womb (trans-abdominal ultrasound). Advantages of trans-abdominal ultrasound include (1) non-invasive longitudinal analysis at different stages, reducing animal use; and (2) maintenance of natural environment, diminishing perturbations on functional parameters, which are more frequent in trans-uterine conditions. Here we describe both approaches, explaining how to identify congenital cardiac defects and defining the correlation between echocardiography findings and histological analysis.For complete details on the use and execution of this protocol, please refer to (Menendez-Montes et al., 2016) and (Menendez-Montes et al., 2021).Ivan Menendez-MontesMaria Villalba-OreroBeatriz EscobarSilvia Martin-PuigElsevierarticleDevelopmental biologyHealth SciencesModel OrganismsScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100980- (2021) |
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Developmental biology Health Sciences Model Organisms Science (General) Q1-390 |
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Developmental biology Health Sciences Model Organisms Science (General) Q1-390 Ivan Menendez-Montes Maria Villalba-Orero Beatriz Escobar Silvia Martin-Puig Embryonic echocardiography for assessment of congenital and functional cardiac defects |
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Summary: Cardiac function and morphology by mouse fetal echocardiography can be assessed by scanning the uterus extracted from the abdominal cavity (trans-uterine ultrasound) or the womb (trans-abdominal ultrasound). Advantages of trans-abdominal ultrasound include (1) non-invasive longitudinal analysis at different stages, reducing animal use; and (2) maintenance of natural environment, diminishing perturbations on functional parameters, which are more frequent in trans-uterine conditions. Here we describe both approaches, explaining how to identify congenital cardiac defects and defining the correlation between echocardiography findings and histological analysis.For complete details on the use and execution of this protocol, please refer to (Menendez-Montes et al., 2016) and (Menendez-Montes et al., 2021). |
format |
article |
author |
Ivan Menendez-Montes Maria Villalba-Orero Beatriz Escobar Silvia Martin-Puig |
author_facet |
Ivan Menendez-Montes Maria Villalba-Orero Beatriz Escobar Silvia Martin-Puig |
author_sort |
Ivan Menendez-Montes |
title |
Embryonic echocardiography for assessment of congenital and functional cardiac defects |
title_short |
Embryonic echocardiography for assessment of congenital and functional cardiac defects |
title_full |
Embryonic echocardiography for assessment of congenital and functional cardiac defects |
title_fullStr |
Embryonic echocardiography for assessment of congenital and functional cardiac defects |
title_full_unstemmed |
Embryonic echocardiography for assessment of congenital and functional cardiac defects |
title_sort |
embryonic echocardiography for assessment of congenital and functional cardiac defects |
publisher |
Elsevier |
publishDate |
2021 |
url |
https://doaj.org/article/406f90a7994f4b2f87388009d7e6dcd2 |
work_keys_str_mv |
AT ivanmenendezmontes embryonicechocardiographyforassessmentofcongenitalandfunctionalcardiacdefects AT mariavillalbaorero embryonicechocardiographyforassessmentofcongenitalandfunctionalcardiacdefects AT beatrizescobar embryonicechocardiographyforassessmentofcongenitalandfunctionalcardiacdefects AT silviamartinpuig embryonicechocardiographyforassessmentofcongenitalandfunctionalcardiacdefects |
_version_ |
1718372907123474432 |