Secretome Analysis of Rabbit and Human Mesenchymal Stem and Endothelial Progenitor Cells: A Comparative Study

Human adipose tissue-derived mesenchymal stem cells (AT-MSCs) have been studied several years for their immunomodulatory effect through the paracrine mechanism and cytokine secretion. In combination with endothelial progenitor cells (EPCs), MSCs have great therapeutical potential for the repair of e...

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Autores principales:	Jaromír Vašíček, Andrej Baláži, Mária Tirpáková, Andrea Svoradová, Ľubomír Ondruška, Vladimír Parkányi, Peter Chrenek
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	rabbit adipose tissue MSCs EPCs cytokine array Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/406fbe02830b496b8d717017969eed87
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spelling	oai:doaj.org-article:406fbe02830b496b8d717017969eed872021-11-25T17:55:01ZSecretome Analysis of Rabbit and Human Mesenchymal Stem and Endothelial Progenitor Cells: A Comparative Study10.3390/ijms2222122831422-00671661-6596https://doaj.org/article/406fbe02830b496b8d717017969eed872021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12283https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Human adipose tissue-derived mesenchymal stem cells (AT-MSCs) have been studied several years for their immunomodulatory effect through the paracrine mechanism and cytokine secretion. In combination with endothelial progenitor cells (EPCs), MSCs have great therapeutical potential for the repair of endothelium and wound healing. However, little is known about the cytokine profile of rabbit AT-MSCs or even EPCs. The aim of this study was to analyze the secretomes of these rabbit stem/progenitor cells. A large-scale human cytokine array (up to 80 cytokines) was used to identify and compare cytokines secreted into conditioned media of human and rabbit AT-MSCs as well as HUVECs and rabbit EPCs. Few cytokines were highly expressed by human AT-MSCs (TIMP-2, TIMP-1), HUVECs (MCP-1, TIMP-2, GRO, Angiogenin, IL-8, TIMP-1), or by rabbit EPCs (TIMP-2). Several cytokines have moderate expression by human (MCP-1, GRO, Angiogenin, TGF-β 2, IL-8, LIF, IL-6, Osteopontin, Osteoprotegerin) and rabbit AT-MSCs (TIMP-2, TGF-β 2, LIF, Osteopontin, IL-8, IL-5, IL-3) or by HUVECs (IL-6, MIF, TGF-β 2, GCP-2, IGFBP-2, Osteoprotegerin, EGF, LIF, PDGF-BB, MCP-3, Osteopontin, Leptin, IL-5, ENA-78, TNF-β) and rabbit EPCs (TGF-β 2, Osteopontin, GRO, LIF, IL-8, IL-5, IL-3). In conclusion, the proposed method seems to be useful for the secretome analysis of rabbit stem/progenitor cells.Jaromír VašíčekAndrej BalážiMária TirpákováAndrea SvoradováĽubomír OndruškaVladimír ParkányiPeter ChrenekMDPI AGarticlerabbitadipose tissueMSCsEPCscytokine arrayBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12283, p 12283 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rabbit adipose tissue MSCs EPCs cytokine array Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	rabbit adipose tissue MSCs EPCs cytokine array Biology (General) QH301-705.5 Chemistry QD1-999 Jaromír Vašíček Andrej Baláži Mária Tirpáková Andrea Svoradová Ľubomír Ondruška Vladimír Parkányi Peter Chrenek Secretome Analysis of Rabbit and Human Mesenchymal Stem and Endothelial Progenitor Cells: A Comparative Study
description	Human adipose tissue-derived mesenchymal stem cells (AT-MSCs) have been studied several years for their immunomodulatory effect through the paracrine mechanism and cytokine secretion. In combination with endothelial progenitor cells (EPCs), MSCs have great therapeutical potential for the repair of endothelium and wound healing. However, little is known about the cytokine profile of rabbit AT-MSCs or even EPCs. The aim of this study was to analyze the secretomes of these rabbit stem/progenitor cells. A large-scale human cytokine array (up to 80 cytokines) was used to identify and compare cytokines secreted into conditioned media of human and rabbit AT-MSCs as well as HUVECs and rabbit EPCs. Few cytokines were highly expressed by human AT-MSCs (TIMP-2, TIMP-1), HUVECs (MCP-1, TIMP-2, GRO, Angiogenin, IL-8, TIMP-1), or by rabbit EPCs (TIMP-2). Several cytokines have moderate expression by human (MCP-1, GRO, Angiogenin, TGF-β 2, IL-8, LIF, IL-6, Osteopontin, Osteoprotegerin) and rabbit AT-MSCs (TIMP-2, TGF-β 2, LIF, Osteopontin, IL-8, IL-5, IL-3) or by HUVECs (IL-6, MIF, TGF-β 2, GCP-2, IGFBP-2, Osteoprotegerin, EGF, LIF, PDGF-BB, MCP-3, Osteopontin, Leptin, IL-5, ENA-78, TNF-β) and rabbit EPCs (TGF-β 2, Osteopontin, GRO, LIF, IL-8, IL-5, IL-3). In conclusion, the proposed method seems to be useful for the secretome analysis of rabbit stem/progenitor cells.
format	article
author	Jaromír Vašíček Andrej Baláži Mária Tirpáková Andrea Svoradová Ľubomír Ondruška Vladimír Parkányi Peter Chrenek
author_facet	Jaromír Vašíček Andrej Baláži Mária Tirpáková Andrea Svoradová Ľubomír Ondruška Vladimír Parkányi Peter Chrenek
author_sort	Jaromír Vašíček
title	Secretome Analysis of Rabbit and Human Mesenchymal Stem and Endothelial Progenitor Cells: A Comparative Study
title_short	Secretome Analysis of Rabbit and Human Mesenchymal Stem and Endothelial Progenitor Cells: A Comparative Study
title_full	Secretome Analysis of Rabbit and Human Mesenchymal Stem and Endothelial Progenitor Cells: A Comparative Study
title_fullStr	Secretome Analysis of Rabbit and Human Mesenchymal Stem and Endothelial Progenitor Cells: A Comparative Study
title_full_unstemmed	Secretome Analysis of Rabbit and Human Mesenchymal Stem and Endothelial Progenitor Cells: A Comparative Study
title_sort	secretome analysis of rabbit and human mesenchymal stem and endothelial progenitor cells: a comparative study
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/406fbe02830b496b8d717017969eed87
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Secretome Analysis of Rabbit and Human Mesenchymal Stem and Endothelial Progenitor Cells: A Comparative Study

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