Physiological and Transcriptomic Responses to Nitrogen Deficiency in Neolamarckia cadamba

Nitrogen (N) is one of the abundant and essential elements for plant growth and development, and N deficiency (ND) affects plants at both physiological and transcriptomic levels. Neolamarckia cadamba is a fast-growing woody plant from the Rubiaceae family. However, the physiological and molecular im...

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Autores principales:	Lu Lu, Yuanyuan Zhang, Lu Li, Na Yi, Yi Liu, Mirza Faisal Qaseem, Huiling Li, Ai-Min Wu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	nitrogen deficiency Neolamarckia cadamba transcriptome analysis physiology stress response Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/40702b72e0fe4a3a95dcf09ab92ed6b3
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spelling	oai:doaj.org-article:40702b72e0fe4a3a95dcf09ab92ed6b32021-11-30T12:21:07ZPhysiological and Transcriptomic Responses to Nitrogen Deficiency in Neolamarckia cadamba1664-462X10.3389/fpls.2021.747121https://doaj.org/article/40702b72e0fe4a3a95dcf09ab92ed6b32021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.747121/fullhttps://doaj.org/toc/1664-462XNitrogen (N) is one of the abundant and essential elements for plant growth and development, and N deficiency (ND) affects plants at both physiological and transcriptomic levels. Neolamarckia cadamba is a fast-growing woody plant from the Rubiaceae family. However, the physiological and molecular impacts of ND on this species have not been well investigated. Here, we studied how N. cadamba responds to ND under hydroponic conditions. In a physiological aspect, ND led to a reduction in biomass, chlorophyll content, and photosynthetic capacity. ND also impaired the assimilation of N as the activities of glutamine synthetase (GS) and nitrate reductase (NR) were decreased in the root. Interestingly, the lignin content of stem increased progressively during the ND stress. The main transcription factors, the transcription factors that are important to N regulation has been found to be upregulated, including Nodule inception-like protein 7 (NLP7), TGACG motif-binding factor 1 (TGA1), basic helix-loop-helix protein 45 (BHLH45), NAM, ATAF1,2, CUC2 (NAC) transcription factor 43 (NAC43), and basic leucine zipper pattern 44 (bZIP44). The expression of N transporters, such as nitrate transporter 2.4 (NRT2.4), ammonium transporter 3 (AMT3), and amino acid transporter protein 3 (AAP3), was also upregulated. In addition, phosphorus- and calcium-related genes such as phosphate starvation response 2 (PHR2) and cyclic nucleotide-gated ion channel 15 (CNGC15) were expressed more abundantly in response to ND stress. Our results reveal the physiological and molecular mechanisms by which woody plants respond to ND.Lu LuLu LuYuanyuan ZhangYuanyuan ZhangLu LiLu LiNa YiNa YiYi LiuYi LiuMirza Faisal QaseemMirza Faisal QaseemHuiling LiHuiling LiAi-Min WuAi-Min WuAi-Min WuFrontiers Media S.A.articlenitrogen deficiencyNeolamarckia cadambatranscriptome analysisphysiologystress responsePlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nitrogen deficiency Neolamarckia cadamba transcriptome analysis physiology stress response Plant culture SB1-1110
spellingShingle	nitrogen deficiency Neolamarckia cadamba transcriptome analysis physiology stress response Plant culture SB1-1110 Lu Lu Lu Lu Yuanyuan Zhang Yuanyuan Zhang Lu Li Lu Li Na Yi Na Yi Yi Liu Yi Liu Mirza Faisal Qaseem Mirza Faisal Qaseem Huiling Li Huiling Li Ai-Min Wu Ai-Min Wu Ai-Min Wu Physiological and Transcriptomic Responses to Nitrogen Deficiency in Neolamarckia cadamba
description	Nitrogen (N) is one of the abundant and essential elements for plant growth and development, and N deficiency (ND) affects plants at both physiological and transcriptomic levels. Neolamarckia cadamba is a fast-growing woody plant from the Rubiaceae family. However, the physiological and molecular impacts of ND on this species have not been well investigated. Here, we studied how N. cadamba responds to ND under hydroponic conditions. In a physiological aspect, ND led to a reduction in biomass, chlorophyll content, and photosynthetic capacity. ND also impaired the assimilation of N as the activities of glutamine synthetase (GS) and nitrate reductase (NR) were decreased in the root. Interestingly, the lignin content of stem increased progressively during the ND stress. The main transcription factors, the transcription factors that are important to N regulation has been found to be upregulated, including Nodule inception-like protein 7 (NLP7), TGACG motif-binding factor 1 (TGA1), basic helix-loop-helix protein 45 (BHLH45), NAM, ATAF1,2, CUC2 (NAC) transcription factor 43 (NAC43), and basic leucine zipper pattern 44 (bZIP44). The expression of N transporters, such as nitrate transporter 2.4 (NRT2.4), ammonium transporter 3 (AMT3), and amino acid transporter protein 3 (AAP3), was also upregulated. In addition, phosphorus- and calcium-related genes such as phosphate starvation response 2 (PHR2) and cyclic nucleotide-gated ion channel 15 (CNGC15) were expressed more abundantly in response to ND stress. Our results reveal the physiological and molecular mechanisms by which woody plants respond to ND.
format	article
author	Lu Lu Lu Lu Yuanyuan Zhang Yuanyuan Zhang Lu Li Lu Li Na Yi Na Yi Yi Liu Yi Liu Mirza Faisal Qaseem Mirza Faisal Qaseem Huiling Li Huiling Li Ai-Min Wu Ai-Min Wu Ai-Min Wu
author_facet	Lu Lu Lu Lu Yuanyuan Zhang Yuanyuan Zhang Lu Li Lu Li Na Yi Na Yi Yi Liu Yi Liu Mirza Faisal Qaseem Mirza Faisal Qaseem Huiling Li Huiling Li Ai-Min Wu Ai-Min Wu Ai-Min Wu
author_sort	Lu Lu
title	Physiological and Transcriptomic Responses to Nitrogen Deficiency in Neolamarckia cadamba
title_short	Physiological and Transcriptomic Responses to Nitrogen Deficiency in Neolamarckia cadamba
title_full	Physiological and Transcriptomic Responses to Nitrogen Deficiency in Neolamarckia cadamba
title_fullStr	Physiological and Transcriptomic Responses to Nitrogen Deficiency in Neolamarckia cadamba
title_full_unstemmed	Physiological and Transcriptomic Responses to Nitrogen Deficiency in Neolamarckia cadamba
title_sort	physiological and transcriptomic responses to nitrogen deficiency in neolamarckia cadamba
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/40702b72e0fe4a3a95dcf09ab92ed6b3
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Physiological and Transcriptomic Responses to Nitrogen Deficiency in Neolamarckia cadamba

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