Rat model of nonalcoholic steatohepatitis created by methionine and choline deficiency: biochemical and histological analyses

Shinichi Nagai1, Jun Iwamoto2, Masakazu Suzuki1, Azusa Seki11Hamri Co Ltd, Koga, Ibaraki, Japan; 2Institute for Integrated Sports Medicine, Keio University School of Medicine, Shinjuku-ku, Tokyo, JapanBackground: The purpose of this study was to establish a Sprague-Dawley rat model of nonalcoholic s...

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Autores principales:	Seki A, Suzuki M, Iwamoto J, Nagai S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/407a1002736447a69da788bdfb1f42ff
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spelling	oai:doaj.org-article:407a1002736447a69da788bdfb1f42ff2021-12-02T01:26:15ZRat model of nonalcoholic steatohepatitis created by methionine and choline deficiency: biochemical and histological analyses2230-2034https://doaj.org/article/407a1002736447a69da788bdfb1f42ff2011-07-01T00:00:00Zhttp://www.dovepress.com/rat-model-of-nonalcoholic-steatohepatitis-created-by-methionine-and-ch-a7892https://doaj.org/toc/2230-2034Shinichi Nagai1, Jun Iwamoto2, Masakazu Suzuki1, Azusa Seki11Hamri Co Ltd, Koga, Ibaraki, Japan; 2Institute for Integrated Sports Medicine, Keio University School of Medicine, Shinjuku-ku, Tokyo, JapanBackground: The purpose of this study was to establish a Sprague-Dawley rat model of nonalcoholic steatohepatitis (NASH) due to combined methionine and choline deficiency (MCD). Methods: Eighty nine-week-old male Sprague-Dawley rats were randomized into two groups (n = 40), comprising an MCD diet group and a standard diet (control) group. After fasting blood was collected, 10 rats from each group were scheduled to be sacrificed at weeks 4, 8, 12, and 16 from the start of the experiment. Body weight and liver wet weight were measured, and histological examination of the liver was performed after hematoxylin and eosin and Oil Red O staining. Results: In the MCD group, body weight and liver wet weight were decreased compared with the control group, while serum levels of albumin, &gamma;-glutamyltranspeptidase, alkaline phosphatase, and total bilirubin were increased, but serum levels of total cholesterol and triglycerides were decreased. Histological examination of the liver revealed centrilobular hepatocellular fatty change from as early as four weeks, with mild fibrosis after 12 weeks. Conclusion: These findings suggested the onset of NASH with liver dysfunction and bile duct damage in rats fed with the MCD diet. Increased fatty acid uptake and decreased cholesterol secretion were considered to be important mechanisms by which the MCD diet promoted intrahepatic lipid accumulation in this model.Keywords: nonalcoholic steatohepatitis, rat, methionine, choline, fatty liver&nbsp;Seki ASuzuki MIwamoto JNagai SDove Medical PressarticleVeterinary medicineSF600-1100ENVeterinary Medicine: Research and Reports, Vol 2011, Iss default, Pp 1-8 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Veterinary medicine SF600-1100
spellingShingle	Veterinary medicine SF600-1100 Seki A Suzuki M Iwamoto J Nagai S Rat model of nonalcoholic steatohepatitis created by methionine and choline deficiency: biochemical and histological analyses
description	Shinichi Nagai1, Jun Iwamoto2, Masakazu Suzuki1, Azusa Seki11Hamri Co Ltd, Koga, Ibaraki, Japan; 2Institute for Integrated Sports Medicine, Keio University School of Medicine, Shinjuku-ku, Tokyo, JapanBackground: The purpose of this study was to establish a Sprague-Dawley rat model of nonalcoholic steatohepatitis (NASH) due to combined methionine and choline deficiency (MCD). Methods: Eighty nine-week-old male Sprague-Dawley rats were randomized into two groups (n = 40), comprising an MCD diet group and a standard diet (control) group. After fasting blood was collected, 10 rats from each group were scheduled to be sacrificed at weeks 4, 8, 12, and 16 from the start of the experiment. Body weight and liver wet weight were measured, and histological examination of the liver was performed after hematoxylin and eosin and Oil Red O staining. Results: In the MCD group, body weight and liver wet weight were decreased compared with the control group, while serum levels of albumin, &gamma;-glutamyltranspeptidase, alkaline phosphatase, and total bilirubin were increased, but serum levels of total cholesterol and triglycerides were decreased. Histological examination of the liver revealed centrilobular hepatocellular fatty change from as early as four weeks, with mild fibrosis after 12 weeks. Conclusion: These findings suggested the onset of NASH with liver dysfunction and bile duct damage in rats fed with the MCD diet. Increased fatty acid uptake and decreased cholesterol secretion were considered to be important mechanisms by which the MCD diet promoted intrahepatic lipid accumulation in this model.Keywords: nonalcoholic steatohepatitis, rat, methionine, choline, fatty liver&nbsp;
format	article
author	Seki A Suzuki M Iwamoto J Nagai S
author_facet	Seki A Suzuki M Iwamoto J Nagai S
author_sort	Seki A
title	Rat model of nonalcoholic steatohepatitis created by methionine and choline deficiency: biochemical and histological analyses
title_short	Rat model of nonalcoholic steatohepatitis created by methionine and choline deficiency: biochemical and histological analyses
title_full	Rat model of nonalcoholic steatohepatitis created by methionine and choline deficiency: biochemical and histological analyses
title_fullStr	Rat model of nonalcoholic steatohepatitis created by methionine and choline deficiency: biochemical and histological analyses
title_full_unstemmed	Rat model of nonalcoholic steatohepatitis created by methionine and choline deficiency: biochemical and histological analyses
title_sort	rat model of nonalcoholic steatohepatitis created by methionine and choline deficiency: biochemical and histological analyses
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/407a1002736447a69da788bdfb1f42ff
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Rat model of nonalcoholic steatohepatitis created by methionine and choline deficiency: biochemical and histological analyses

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