Molecular identification of bacteria isolated from culture medium of the gold-lipped pearl oyster Pinctada maxima larvae

Abstract. Wullur S, Napitupulu H, Wantania LL, Ginting EL, Mamangkey NGF, Smolak R, Ogello E. 2020. Molecular identification of bacteria isolated from culture medium of the gold-lipped pearl oyster Pinctada maxima larvae. Biodiversitas 21: 5291-5297. This study was conducted for the molecular ident...

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Autores principales:	Stenly Wullur, HATOPAN NAPITUPULU, ELVY LIKE GINTING, NOLDY GUSTAF FRANS MAMANGKEY, LETHA LOUISIANAWANTANIA, RADOSLAV SMOLAK, ERICK OGELLO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	16s rrna, bacteria, fish-waste diet, pinctada maxima, gold-lipped pearl oyster Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/407ea37bff9d4cbabe23c2fc357effc0
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spelling	oai:doaj.org-article:407ea37bff9d4cbabe23c2fc357effc02021-11-22T00:44:11ZMolecular identification of bacteria isolated from culture medium of the gold-lipped pearl oyster Pinctada maxima larvae1412-033X2085-472210.13057/biodiv/d211133https://doaj.org/article/407ea37bff9d4cbabe23c2fc357effc02020-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6530https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Wullur S, Napitupulu H, Wantania LL, Ginting EL, Mamangkey NGF, Smolak R, Ogello E. 2020. Molecular identification of bacteria isolated from culture medium of the gold-lipped pearl oyster Pinctada maxima larvae. Biodiversitas 21: 5291-5297. This study was conducted for the molecular identification of bacteria species isolated from culture medium of the gold-lipped pearl oyster, Pinctada maxima larvae. The pearl oysters were cultured using live-microalgae (Isochrysis sp) and fish waste diet (FWD) as food sources. Bacteria were isolated from the oyster larvae and identified based on the 16S rRNA gene sequence. The isolated bacteria were grown on agar plates and incubated at 37°C for 24 to 48 hours. Representative colonies of the bacteria were selected and cultured for molecular analysis. The 16S rRNA genes of the bacteria were amplified and the sequences were matched with the NCBI GenBank database. Seven different colonies were observed based on morphological characters. Similarity test by conducting the Basic Local Alignment Search Tool (BLAST) in the NCBI GenBank database, using the 16S rRNA gene sequences showed that the seven isolates colonies possess high similarity to five bacteria species i.e. Pseudomonas pachastrellae, Vibrio alginolyticus, Bacillus filamentosus, Bacillus cereus and Idiomarina fontislapidosi belonging to four different genera i.e. Bacillus, Staphylococcus, Vibrio, and Alteromonas.Stenly WullurHATOPAN NAPITUPULUELVY LIKE GINTINGNOLDY GUSTAF FRANS MAMANGKEYLETHA LOUISIANAWANTANIARADOSLAV SMOLAKERICK OGELLOMBI & UNS Soloarticle16s rrna, bacteria, fish-waste diet, pinctada maxima, gold-lipped pearl oysterBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	16s rrna, bacteria, fish-waste diet, pinctada maxima, gold-lipped pearl oyster Biology (General) QH301-705.5
spellingShingle	16s rrna, bacteria, fish-waste diet, pinctada maxima, gold-lipped pearl oyster Biology (General) QH301-705.5 Stenly Wullur HATOPAN NAPITUPULU ELVY LIKE GINTING NOLDY GUSTAF FRANS MAMANGKEY LETHA LOUISIANAWANTANIA RADOSLAV SMOLAK ERICK OGELLO Molecular identification of bacteria isolated from culture medium of the gold-lipped pearl oyster Pinctada maxima larvae
description	Abstract. Wullur S, Napitupulu H, Wantania LL, Ginting EL, Mamangkey NGF, Smolak R, Ogello E. 2020. Molecular identification of bacteria isolated from culture medium of the gold-lipped pearl oyster Pinctada maxima larvae. Biodiversitas 21: 5291-5297. This study was conducted for the molecular identification of bacteria species isolated from culture medium of the gold-lipped pearl oyster, Pinctada maxima larvae. The pearl oysters were cultured using live-microalgae (Isochrysis sp) and fish waste diet (FWD) as food sources. Bacteria were isolated from the oyster larvae and identified based on the 16S rRNA gene sequence. The isolated bacteria were grown on agar plates and incubated at 37°C for 24 to 48 hours. Representative colonies of the bacteria were selected and cultured for molecular analysis. The 16S rRNA genes of the bacteria were amplified and the sequences were matched with the NCBI GenBank database. Seven different colonies were observed based on morphological characters. Similarity test by conducting the Basic Local Alignment Search Tool (BLAST) in the NCBI GenBank database, using the 16S rRNA gene sequences showed that the seven isolates colonies possess high similarity to five bacteria species i.e. Pseudomonas pachastrellae, Vibrio alginolyticus, Bacillus filamentosus, Bacillus cereus and Idiomarina fontislapidosi belonging to four different genera i.e. Bacillus, Staphylococcus, Vibrio, and Alteromonas.
format	article
author	Stenly Wullur HATOPAN NAPITUPULU ELVY LIKE GINTING NOLDY GUSTAF FRANS MAMANGKEY LETHA LOUISIANAWANTANIA RADOSLAV SMOLAK ERICK OGELLO
author_facet	Stenly Wullur HATOPAN NAPITUPULU ELVY LIKE GINTING NOLDY GUSTAF FRANS MAMANGKEY LETHA LOUISIANAWANTANIA RADOSLAV SMOLAK ERICK OGELLO
author_sort	Stenly Wullur
title	Molecular identification of bacteria isolated from culture medium of the gold-lipped pearl oyster Pinctada maxima larvae
title_short	Molecular identification of bacteria isolated from culture medium of the gold-lipped pearl oyster Pinctada maxima larvae
title_full	Molecular identification of bacteria isolated from culture medium of the gold-lipped pearl oyster Pinctada maxima larvae
title_fullStr	Molecular identification of bacteria isolated from culture medium of the gold-lipped pearl oyster Pinctada maxima larvae
title_full_unstemmed	Molecular identification of bacteria isolated from culture medium of the gold-lipped pearl oyster Pinctada maxima larvae
title_sort	molecular identification of bacteria isolated from culture medium of the gold-lipped pearl oyster pinctada maxima larvae
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/407ea37bff9d4cbabe23c2fc357effc0
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Molecular identification of bacteria isolated from culture medium of the gold-lipped pearl oyster Pinctada maxima larvae

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