Blockage of both the extrinsic and intrinsic pathways of diazinon-induced apoptosis in PaTu cells by magnesium oxide and selenium nanoparticles

Mahdi Shiri,1,2,* Mona Navaei-Nigjeh,1,3,* Maryam Baeeri,1 Mahban Rahimifard,1 Hossein Mahboudi,4 Ahmad Reza Shahverdi,5 Abbas Kebriaeezadeh,1 Mohammad Abdollahi1,6,7 1Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Me...

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Autores principales:	Shiri M, Navaei-Nigjeh M, Baeeri M, Rahimifard M, Mahboudi H, Shahverdi AR, Kebriaeezadeh A, Abdollahi M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Apoptosis Diazinon Human pancreatic cancer cell line Organophosphorus Toxicity Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4086fe75fa19404b9321e782094a4014
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spelling	oai:doaj.org-article:4086fe75fa19404b9321e782094a40142021-12-02T03:54:12ZBlockage of both the extrinsic and intrinsic pathways of diazinon-induced apoptosis in PaTu cells by magnesium oxide and selenium nanoparticles1178-2013https://doaj.org/article/4086fe75fa19404b9321e782094a40142016-11-01T00:00:00Zhttps://www.dovepress.com/blockage-of-both-the-extrinsic-and-intrinsic-pathways-of-diazinon-indu-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Mahdi Shiri,1,2,* Mona Navaei-Nigjeh,1,3,* Maryam Baeeri,1 Mahban Rahimifard,1 Hossein Mahboudi,4 Ahmad Reza Shahverdi,5 Abbas Kebriaeezadeh,1 Mohammad Abdollahi1,6,7 1Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, 2School of Medicine, Artesh University of Medical Sciences, 3Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; 4Department of Biotechnology, Faculty of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 5Department of Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, 6Toxicology Interest Group, USERN, 7Endocrinology & Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran *These authors contributed equally to this work Abstract: Diazinon (DZ) is an organophosphorus insecticide that acts as an acetylcholinesterase inhibitor. It is important to note that it can induce oxidative stress, lipid peroxidation, diabetic disorders, and cytotoxicity. Magnesium oxide (MgO) and selenium nanoparticles (Se NPs) showed promising protection against oxidative stress, lipid peroxidation, cytotoxicity, and diabetic disorders. Therefore, this study was conducted to explore the possible protective mechanisms of MgO and Se NPs against DZ-induced cytotoxicity in PaTu cell line. Cytotoxicity of DZ, in the presence or absence of effective doses of MgO and Se NPs, was determined in human pancreatic cancer cell line (PaTu cells) after 24 hours of exposure by using mitochondrial activity and mitochondrial membrane potential assays. Then, the insulin, proinsulin, and C-peptide release; caspase-3 and -9 activities; and total thiol molecule levels were assessed. Determination of cell viability, including apoptotic and necrotic cells, was assessed via acridine orange/ethidium bromide double staining. Furthermore, expression of 15 genes associated with cell death/apoptosis in various phenomena was examined after 24 hours of contact with DZ and NPs by using real-time polymerase chain reaction. Compared to the individual cases, the group receiving the combination of MgO and Se NPs showed more beneficial effects in reducing the toxicity of DZ. Cotreatment of PaTu cell lines with MgO and Se NPs counteracts the toxicity of DZ on insulin-producing cells. Keywords: apoptosis, diazinon, human pancreatic cancer cell line, organophosphorus, toxicityShiri MNavaei-Nigjeh MBaeeri MRahimifard MMahboudi HShahverdi ARKebriaeezadeh AAbdollahi MDove Medical PressarticleApoptosisDiazinonHuman pancreatic cancer cell lineOrganophosphorusToxicityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 6239-6250 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Apoptosis Diazinon Human pancreatic cancer cell line Organophosphorus Toxicity Medicine (General) R5-920
spellingShingle	Apoptosis Diazinon Human pancreatic cancer cell line Organophosphorus Toxicity Medicine (General) R5-920 Shiri M Navaei-Nigjeh M Baeeri M Rahimifard M Mahboudi H Shahverdi AR Kebriaeezadeh A Abdollahi M Blockage of both the extrinsic and intrinsic pathways of diazinon-induced apoptosis in PaTu cells by magnesium oxide and selenium nanoparticles
description	Mahdi Shiri,1,2,* Mona Navaei-Nigjeh,1,3,* Maryam Baeeri,1 Mahban Rahimifard,1 Hossein Mahboudi,4 Ahmad Reza Shahverdi,5 Abbas Kebriaeezadeh,1 Mohammad Abdollahi1,6,7 1Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, 2School of Medicine, Artesh University of Medical Sciences, 3Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; 4Department of Biotechnology, Faculty of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 5Department of Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, 6Toxicology Interest Group, USERN, 7Endocrinology & Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran *These authors contributed equally to this work Abstract: Diazinon (DZ) is an organophosphorus insecticide that acts as an acetylcholinesterase inhibitor. It is important to note that it can induce oxidative stress, lipid peroxidation, diabetic disorders, and cytotoxicity. Magnesium oxide (MgO) and selenium nanoparticles (Se NPs) showed promising protection against oxidative stress, lipid peroxidation, cytotoxicity, and diabetic disorders. Therefore, this study was conducted to explore the possible protective mechanisms of MgO and Se NPs against DZ-induced cytotoxicity in PaTu cell line. Cytotoxicity of DZ, in the presence or absence of effective doses of MgO and Se NPs, was determined in human pancreatic cancer cell line (PaTu cells) after 24 hours of exposure by using mitochondrial activity and mitochondrial membrane potential assays. Then, the insulin, proinsulin, and C-peptide release; caspase-3 and -9 activities; and total thiol molecule levels were assessed. Determination of cell viability, including apoptotic and necrotic cells, was assessed via acridine orange/ethidium bromide double staining. Furthermore, expression of 15 genes associated with cell death/apoptosis in various phenomena was examined after 24 hours of contact with DZ and NPs by using real-time polymerase chain reaction. Compared to the individual cases, the group receiving the combination of MgO and Se NPs showed more beneficial effects in reducing the toxicity of DZ. Cotreatment of PaTu cell lines with MgO and Se NPs counteracts the toxicity of DZ on insulin-producing cells. Keywords: apoptosis, diazinon, human pancreatic cancer cell line, organophosphorus, toxicity
format	article
author	Shiri M Navaei-Nigjeh M Baeeri M Rahimifard M Mahboudi H Shahverdi AR Kebriaeezadeh A Abdollahi M
author_facet	Shiri M Navaei-Nigjeh M Baeeri M Rahimifard M Mahboudi H Shahverdi AR Kebriaeezadeh A Abdollahi M
author_sort	Shiri M
title	Blockage of both the extrinsic and intrinsic pathways of diazinon-induced apoptosis in PaTu cells by magnesium oxide and selenium nanoparticles
title_short	Blockage of both the extrinsic and intrinsic pathways of diazinon-induced apoptosis in PaTu cells by magnesium oxide and selenium nanoparticles
title_full	Blockage of both the extrinsic and intrinsic pathways of diazinon-induced apoptosis in PaTu cells by magnesium oxide and selenium nanoparticles
title_fullStr	Blockage of both the extrinsic and intrinsic pathways of diazinon-induced apoptosis in PaTu cells by magnesium oxide and selenium nanoparticles
title_full_unstemmed	Blockage of both the extrinsic and intrinsic pathways of diazinon-induced apoptosis in PaTu cells by magnesium oxide and selenium nanoparticles
title_sort	blockage of both the extrinsic and intrinsic pathways of diazinon-induced apoptosis in patu cells by magnesium oxide and selenium nanoparticles
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/4086fe75fa19404b9321e782094a4014
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Blockage of both the extrinsic and intrinsic pathways of diazinon-induced apoptosis in PaTu cells by magnesium oxide and selenium nanoparticles

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