Faecal biomarkers in type 1 diabetes with and without diabetic nephropathy

Abstract Gastrointestinal dysbiosis is common among persons with type 1 diabetes (T1D), but its potential impact on diabetic nephropathy (DN) remains obscure. We examined whether faecal biomarkers, previously associated with low-grade gastrointestinal inflammation, differ between healthy controls an...

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Autores principales:	Signe Abitz Winther, Miia Maininki Mannerla, Marie Frimodt-Møller, Frederik Persson, Tine Willum Hansen, Markku Lehto, Sohvi Hörkkö, Michael Blaut, Carol Forsblom, Per-Henrik Groop, Peter Rossing
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/408992ee5356447c8bc81c5b73641639
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spelling	oai:doaj.org-article:408992ee5356447c8bc81c5b736416392021-12-02T16:24:59ZFaecal biomarkers in type 1 diabetes with and without diabetic nephropathy10.1038/s41598-021-94747-82045-2322https://doaj.org/article/408992ee5356447c8bc81c5b736416392021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94747-8https://doaj.org/toc/2045-2322Abstract Gastrointestinal dysbiosis is common among persons with type 1 diabetes (T1D), but its potential impact on diabetic nephropathy (DN) remains obscure. We examined whether faecal biomarkers, previously associated with low-grade gastrointestinal inflammation, differ between healthy controls and T1D subjects with and without DN. Faecal samples were analyzed for levels of calprotectin, intestinal alkaline phosphatase (IAP), short-chain fatty acids (SCFA) and immunoglobulins in subjects with T1D (n = 159) and healthy controls (NDC; n = 50). The subjects with T1D were stratified based on albuminuria: normoalbuminuria (< 30 mg/g; n = 49), microalbuminuria (30–299 mg/g; n = 50) and macroalbuminuria (≥ 300 mg/g; n = 60). aecal calprotectin, IAP and immunoglobulin levels did not differ between the T1D albuminuria groups. However, when subjects were stratified based on faecal calprotectin cut-off level (50 µg/g), macroalbuminuric T1D subjects exceeded the threshold more frequently than NDC (p = 0.02). Concentrations of faecal propionate and butyrate were lower in T1D subjects compared with NDC (p = 0.04 and p = 0.03, respectively). Among T1D subjects, levels of branched SCFA (BCFA) correlated positively with current albuminuria level (isobutyrate, p = 0.03; isovalerate, p = 0.005). In our study cohort, fatty acid metabolism seemed to be altered among T1D subjects and those with albuminuria compared to NDC. This may reflect gastrointestinal imbalances associated with T1D and renal complications.Signe Abitz WintherMiia Maininki MannerlaMarie Frimodt-MøllerFrederik PerssonTine Willum HansenMarkku LehtoSohvi HörkköMichael BlautCarol ForsblomPer-Henrik GroopPeter RossingNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Signe Abitz Winther Miia Maininki Mannerla Marie Frimodt-Møller Frederik Persson Tine Willum Hansen Markku Lehto Sohvi Hörkkö Michael Blaut Carol Forsblom Per-Henrik Groop Peter Rossing Faecal biomarkers in type 1 diabetes with and without diabetic nephropathy
description	Abstract Gastrointestinal dysbiosis is common among persons with type 1 diabetes (T1D), but its potential impact on diabetic nephropathy (DN) remains obscure. We examined whether faecal biomarkers, previously associated with low-grade gastrointestinal inflammation, differ between healthy controls and T1D subjects with and without DN. Faecal samples were analyzed for levels of calprotectin, intestinal alkaline phosphatase (IAP), short-chain fatty acids (SCFA) and immunoglobulins in subjects with T1D (n = 159) and healthy controls (NDC; n = 50). The subjects with T1D were stratified based on albuminuria: normoalbuminuria (< 30 mg/g; n = 49), microalbuminuria (30–299 mg/g; n = 50) and macroalbuminuria (≥ 300 mg/g; n = 60). aecal calprotectin, IAP and immunoglobulin levels did not differ between the T1D albuminuria groups. However, when subjects were stratified based on faecal calprotectin cut-off level (50 µg/g), macroalbuminuric T1D subjects exceeded the threshold more frequently than NDC (p = 0.02). Concentrations of faecal propionate and butyrate were lower in T1D subjects compared with NDC (p = 0.04 and p = 0.03, respectively). Among T1D subjects, levels of branched SCFA (BCFA) correlated positively with current albuminuria level (isobutyrate, p = 0.03; isovalerate, p = 0.005). In our study cohort, fatty acid metabolism seemed to be altered among T1D subjects and those with albuminuria compared to NDC. This may reflect gastrointestinal imbalances associated with T1D and renal complications.
format	article
author	Signe Abitz Winther Miia Maininki Mannerla Marie Frimodt-Møller Frederik Persson Tine Willum Hansen Markku Lehto Sohvi Hörkkö Michael Blaut Carol Forsblom Per-Henrik Groop Peter Rossing
author_facet	Signe Abitz Winther Miia Maininki Mannerla Marie Frimodt-Møller Frederik Persson Tine Willum Hansen Markku Lehto Sohvi Hörkkö Michael Blaut Carol Forsblom Per-Henrik Groop Peter Rossing
author_sort	Signe Abitz Winther
title	Faecal biomarkers in type 1 diabetes with and without diabetic nephropathy
title_short	Faecal biomarkers in type 1 diabetes with and without diabetic nephropathy
title_full	Faecal biomarkers in type 1 diabetes with and without diabetic nephropathy
title_fullStr	Faecal biomarkers in type 1 diabetes with and without diabetic nephropathy
title_full_unstemmed	Faecal biomarkers in type 1 diabetes with and without diabetic nephropathy
title_sort	faecal biomarkers in type 1 diabetes with and without diabetic nephropathy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/408992ee5356447c8bc81c5b73641639
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Faecal biomarkers in type 1 diabetes with and without diabetic nephropathy

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