Rapid plant identification using species- and group-specific primers targeting chloroplast DNA.

Plant identification is challenging when no morphologically assignable parts are available. There is a lack of broadly applicable methods for identifying plants in this situation, for example when roots grow in mixture and for decayed or semi-digested plant material. These difficulties have also imp...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Corinna Wallinger, Anita Juen, Karin Staudacher, Nikolaus Schallhart, Evi Mitterrutzner, Eva-Maria Steiner, Bettina Thalinger, Michael Traugott
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/409895bcc02b4dda8696d2472424c05a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:409895bcc02b4dda8696d2472424c05a
record_format	dspace
spelling	oai:doaj.org-article:409895bcc02b4dda8696d2472424c05a2021-11-18T07:30:23ZRapid plant identification using species- and group-specific primers targeting chloroplast DNA.1932-620310.1371/journal.pone.0029473https://doaj.org/article/409895bcc02b4dda8696d2472424c05a2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22253728/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Plant identification is challenging when no morphologically assignable parts are available. There is a lack of broadly applicable methods for identifying plants in this situation, for example when roots grow in mixture and for decayed or semi-digested plant material. These difficulties have also impeded the progress made in ecological disciplines such as soil- and trophic ecology. Here, a PCR-based approach is presented which allows identifying a variety of plant taxa commonly occurring in Central European agricultural land. Based on the trnT-F cpDNA region, PCR assays were developed to identify two plant families (Poaceae and Apiaceae), the genera Trifolium and Plantago, and nine plant species: Achillea millefolium, Fagopyrum esculentum, Lolium perenne, Lupinus angustifolius, Phaseolus coccineus, Sinapis alba, Taraxacum officinale, Triticum aestivum, and Zea mays. These assays allowed identification of plants based on size-specific amplicons ranging from 116 bp to 381 bp. Their specificity and sensitivity was consistently high, enabling the detection of small amounts of plant DNA, for example, in decaying plant material and in the intestine or faeces of herbivores. To increase the efficacy of identifying plant species from large number of samples, specific primers were combined in multiplex PCRs, allowing screening for multiple species within a single reaction. The molecular assays outlined here will be applicable manifold, such as for root- and leaf litter identification, botanical trace evidence, and the analysis of herbivory.Corinna WallingerAnita JuenKarin StaudacherNikolaus SchallhartEvi MitterrutznerEva-Maria SteinerBettina ThalingerMichael TraugottPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e29473 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Corinna Wallinger Anita Juen Karin Staudacher Nikolaus Schallhart Evi Mitterrutzner Eva-Maria Steiner Bettina Thalinger Michael Traugott Rapid plant identification using species- and group-specific primers targeting chloroplast DNA.
description	Plant identification is challenging when no morphologically assignable parts are available. There is a lack of broadly applicable methods for identifying plants in this situation, for example when roots grow in mixture and for decayed or semi-digested plant material. These difficulties have also impeded the progress made in ecological disciplines such as soil- and trophic ecology. Here, a PCR-based approach is presented which allows identifying a variety of plant taxa commonly occurring in Central European agricultural land. Based on the trnT-F cpDNA region, PCR assays were developed to identify two plant families (Poaceae and Apiaceae), the genera Trifolium and Plantago, and nine plant species: Achillea millefolium, Fagopyrum esculentum, Lolium perenne, Lupinus angustifolius, Phaseolus coccineus, Sinapis alba, Taraxacum officinale, Triticum aestivum, and Zea mays. These assays allowed identification of plants based on size-specific amplicons ranging from 116 bp to 381 bp. Their specificity and sensitivity was consistently high, enabling the detection of small amounts of plant DNA, for example, in decaying plant material and in the intestine or faeces of herbivores. To increase the efficacy of identifying plant species from large number of samples, specific primers were combined in multiplex PCRs, allowing screening for multiple species within a single reaction. The molecular assays outlined here will be applicable manifold, such as for root- and leaf litter identification, botanical trace evidence, and the analysis of herbivory.
format	article
author	Corinna Wallinger Anita Juen Karin Staudacher Nikolaus Schallhart Evi Mitterrutzner Eva-Maria Steiner Bettina Thalinger Michael Traugott
author_facet	Corinna Wallinger Anita Juen Karin Staudacher Nikolaus Schallhart Evi Mitterrutzner Eva-Maria Steiner Bettina Thalinger Michael Traugott
author_sort	Corinna Wallinger
title	Rapid plant identification using species- and group-specific primers targeting chloroplast DNA.
title_short	Rapid plant identification using species- and group-specific primers targeting chloroplast DNA.
title_full	Rapid plant identification using species- and group-specific primers targeting chloroplast DNA.
title_fullStr	Rapid plant identification using species- and group-specific primers targeting chloroplast DNA.
title_full_unstemmed	Rapid plant identification using species- and group-specific primers targeting chloroplast DNA.
title_sort	rapid plant identification using species- and group-specific primers targeting chloroplast dna.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/409895bcc02b4dda8696d2472424c05a
work_keys_str_mv	AT corinnawallinger rapidplantidentificationusingspeciesandgroupspecificprimerstargetingchloroplastdna AT anitajuen rapidplantidentificationusingspeciesandgroupspecificprimerstargetingchloroplastdna AT karinstaudacher rapidplantidentificationusingspeciesandgroupspecificprimerstargetingchloroplastdna AT nikolausschallhart rapidplantidentificationusingspeciesandgroupspecificprimerstargetingchloroplastdna AT evimitterrutzner rapidplantidentificationusingspeciesandgroupspecificprimerstargetingchloroplastdna AT evamariasteiner rapidplantidentificationusingspeciesandgroupspecificprimerstargetingchloroplastdna AT bettinathalinger rapidplantidentificationusingspeciesandgroupspecificprimerstargetingchloroplastdna AT michaeltraugott rapidplantidentificationusingspeciesandgroupspecificprimerstargetingchloroplastdna
_version_	1718423341546602496

Rapid plant identification using species- and group-specific primers targeting chloroplast DNA.

Ejemplares similares