Transgenic Bt rice lines producing Cry1Ac, Cry2Aa or Cry1Ca have no detrimental effects on Brown Planthopper and Pond Wolf Spider

Abstract Transgenic rice expressing cry genes from the bacterium Bacillus thuringiensis (Bt rice) is highly resistant to lepidopteran pests. The brown planthopper (BPH, Nilaparvata lugens) is the main non-target sap-sucking insect pest of Bt transgenic rice. The pond wolf spider (PWS, Pardosa pseudo...

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Autores principales:	Lin Niu, Amani Mannakkara, Lin Qiu, Xiaoping Wang, Hongxia Hua, Chaoliang Lei, Juan Luis Jurat-Fuentes, Weihua Ma
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/40c5a36c94bb499facb4b5f623c5b8ab
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spelling	oai:doaj.org-article:40c5a36c94bb499facb4b5f623c5b8ab2021-12-02T11:40:59ZTransgenic Bt rice lines producing Cry1Ac, Cry2Aa or Cry1Ca have no detrimental effects on Brown Planthopper and Pond Wolf Spider10.1038/s41598-017-02207-z2045-2322https://doaj.org/article/40c5a36c94bb499facb4b5f623c5b8ab2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02207-zhttps://doaj.org/toc/2045-2322Abstract Transgenic rice expressing cry genes from the bacterium Bacillus thuringiensis (Bt rice) is highly resistant to lepidopteran pests. The brown planthopper (BPH, Nilaparvata lugens) is the main non-target sap-sucking insect pest of Bt transgenic rice. The pond wolf spider (PWS, Pardosa pseudoannulata) is one of the most dominant predators of BPH in rice fields. Consequently, the safety evaluation of Bt rice on BPH and PWS should be conducted before commercialization. In the current study, two experiments were performed to assess the potential ecological effects of Bt rice on BPH and PWS: (1) a tritrophic experiment to evaluate the transmission of Cry1Ac, Cry2Aa and Cry1Ca protein in the food chain; and (2) binding assays of Cry1Ac, Cry2Aa and Cry1Ca to midgut brush border membrane proteins from BPH and PWS. Trace amounts of the three Cry proteins were detected in BPH feeding on Bt rice cultivars, but only Cry1Ac and Cry2Aa proteins could be transferred to PWS through feeding on BPH. In vitro binding of biotinylated Cry proteins and competition assays in midgut protein vesicles showed weak binding, and ligand blot analysis confirmed the binding specificity. Thus, we inferred that the tested Bt rice varieties have negligible effects on BPH and PWS.Lin NiuAmani MannakkaraLin QiuXiaoping WangHongxia HuaChaoliang LeiJuan Luis Jurat-FuentesWeihua MaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lin Niu Amani Mannakkara Lin Qiu Xiaoping Wang Hongxia Hua Chaoliang Lei Juan Luis Jurat-Fuentes Weihua Ma Transgenic Bt rice lines producing Cry1Ac, Cry2Aa or Cry1Ca have no detrimental effects on Brown Planthopper and Pond Wolf Spider
description	Abstract Transgenic rice expressing cry genes from the bacterium Bacillus thuringiensis (Bt rice) is highly resistant to lepidopteran pests. The brown planthopper (BPH, Nilaparvata lugens) is the main non-target sap-sucking insect pest of Bt transgenic rice. The pond wolf spider (PWS, Pardosa pseudoannulata) is one of the most dominant predators of BPH in rice fields. Consequently, the safety evaluation of Bt rice on BPH and PWS should be conducted before commercialization. In the current study, two experiments were performed to assess the potential ecological effects of Bt rice on BPH and PWS: (1) a tritrophic experiment to evaluate the transmission of Cry1Ac, Cry2Aa and Cry1Ca protein in the food chain; and (2) binding assays of Cry1Ac, Cry2Aa and Cry1Ca to midgut brush border membrane proteins from BPH and PWS. Trace amounts of the three Cry proteins were detected in BPH feeding on Bt rice cultivars, but only Cry1Ac and Cry2Aa proteins could be transferred to PWS through feeding on BPH. In vitro binding of biotinylated Cry proteins and competition assays in midgut protein vesicles showed weak binding, and ligand blot analysis confirmed the binding specificity. Thus, we inferred that the tested Bt rice varieties have negligible effects on BPH and PWS.
format	article
author	Lin Niu Amani Mannakkara Lin Qiu Xiaoping Wang Hongxia Hua Chaoliang Lei Juan Luis Jurat-Fuentes Weihua Ma
author_facet	Lin Niu Amani Mannakkara Lin Qiu Xiaoping Wang Hongxia Hua Chaoliang Lei Juan Luis Jurat-Fuentes Weihua Ma
author_sort	Lin Niu
title	Transgenic Bt rice lines producing Cry1Ac, Cry2Aa or Cry1Ca have no detrimental effects on Brown Planthopper and Pond Wolf Spider
title_short	Transgenic Bt rice lines producing Cry1Ac, Cry2Aa or Cry1Ca have no detrimental effects on Brown Planthopper and Pond Wolf Spider
title_full	Transgenic Bt rice lines producing Cry1Ac, Cry2Aa or Cry1Ca have no detrimental effects on Brown Planthopper and Pond Wolf Spider
title_fullStr	Transgenic Bt rice lines producing Cry1Ac, Cry2Aa or Cry1Ca have no detrimental effects on Brown Planthopper and Pond Wolf Spider
title_full_unstemmed	Transgenic Bt rice lines producing Cry1Ac, Cry2Aa or Cry1Ca have no detrimental effects on Brown Planthopper and Pond Wolf Spider
title_sort	transgenic bt rice lines producing cry1ac, cry2aa or cry1ca have no detrimental effects on brown planthopper and pond wolf spider
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/40c5a36c94bb499facb4b5f623c5b8ab
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Transgenic Bt rice lines producing Cry1Ac, Cry2Aa or Cry1Ca have no detrimental effects on Brown Planthopper and Pond Wolf Spider

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