Magnetic immunoassay using CdSe/ZnS quantum dots as fluorescent probes to detect the level of DNA methyltransferase 1 in human serum sample

Fei Yu,1,* Ya-min Xiong,1,* Song-cheng Yu,1 Lei-liang He,1 Shan-shan Niu,1 Yu-ming Wu,1 Jie Liu,1 Ling-bo Qu,2 Li-e Liu,1 Yong-jun Wu1 1College of Public Health, 2College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, Henan, People’s Republic of China *These aut...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yu F, Xiong YM, Yu SC, He LL, Niu SS, Wu YM, Liu J, Qu LB, Liu LE, Wu YJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	DNA methyltransferase1 Quantum dots Fluorescence immunoassay Magnetic carboxyl beads Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/40c60ff662e54254b946edc5c2ed2602
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:40c60ff662e54254b946edc5c2ed2602
record_format	dspace
spelling	oai:doaj.org-article:40c60ff662e54254b946edc5c2ed26022021-12-02T02:11:17ZMagnetic immunoassay using CdSe/ZnS quantum dots as fluorescent probes to detect the level of DNA methyltransferase 1 in human serum sample1178-2013https://doaj.org/article/40c60ff662e54254b946edc5c2ed26022018-01-01T00:00:00Zhttps://www.dovepress.com/magnetic-immunoassay-using-cdsezns-quantum-dots-as-fluorescent-probes--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Fei Yu,1,* Ya-min Xiong,1,* Song-cheng Yu,1 Lei-liang He,1 Shan-shan Niu,1 Yu-ming Wu,1 Jie Liu,1 Ling-bo Qu,2 Li-e Liu,1 Yong-jun Wu1 1College of Public Health, 2College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, Henan, People’s Republic of China *These authors contributed equally to this work Background: DNA methyltransferase 1 (DNMT1), a dominant enzyme responsible for the transfer of a methyl group from the universal methyl donor to the 5-position of cytosine residues in DNA, is essential for mammalian development and closely related to cancer and a variety of age-related chronic diseases. DNMT1 has become a useful biomarker in early disease diagnosis and a potential therapeutic target in cancer therapy and drug development. However, till now, most of the studies on DNA methyltransferase (MTase) detection have focused on the prokaryote MTase and its activity.Methods: A magnetic fluorescence-linked immunosorbent assay (FLISA) using CdSe/ZnS quantum dots as fluorescent probes was proposed for the rapid and sensitive detection of the DNMT1 level in this study. Key factors that affect the precision and accuracy of the determination of DNMT1 were optimized.Results: Under the optimal conditions, the limit of detection was 0.1 ng/mL, the linear range was 0.1–1,500 ng/mL, the recovery was 91.67%–106.50%, and the relative standard deviations of intra- and inter-assays were respectively 5.45%–11.29% and 7.03%–11.25%. The cross-reactivity rates with DNA methyltransferases 3a and 3b were only 4.0% and 9.4%, respectively. Furthermore, FLISA was successfully used to detect the levels of DNMT1 in human serum samples, and compared with commercial enzyme-linked immunosorbent assay (ELISA) kits. The results revealed that there was a good correlation between FLISA and commercial ELISA kits (correlation coefficient r=0.866, p=0.001). The linear scope of FLISA was broader than ELISA, and the measurement time was much shorter than ELISA kits.Conclusion: These indicated that the proposed FLISA method was sensitive and high throughput and can quickly screen the level of DNMT1 in serum samples. Keywords: DNA methyltransferase 1, quantum dots, fluorescence immunoassay, magnetic carboxyl beads, high throughput, serum sampleYu FXiong YMYu SCHe LLNiu SSWu YMLiu JQu LBLiu LEWu YJDove Medical PressarticleDNA methyltransferase1Quantum dotsFluorescence immunoassayMagnetic carboxyl beadsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 429-437 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	DNA methyltransferase1 Quantum dots Fluorescence immunoassay Magnetic carboxyl beads Medicine (General) R5-920
spellingShingle	DNA methyltransferase1 Quantum dots Fluorescence immunoassay Magnetic carboxyl beads Medicine (General) R5-920 Yu F Xiong YM Yu SC He LL Niu SS Wu YM Liu J Qu LB Liu LE Wu YJ Magnetic immunoassay using CdSe/ZnS quantum dots as fluorescent probes to detect the level of DNA methyltransferase 1 in human serum sample
description	Fei Yu,1,* Ya-min Xiong,1,* Song-cheng Yu,1 Lei-liang He,1 Shan-shan Niu,1 Yu-ming Wu,1 Jie Liu,1 Ling-bo Qu,2 Li-e Liu,1 Yong-jun Wu1 1College of Public Health, 2College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, Henan, People’s Republic of China *These authors contributed equally to this work Background: DNA methyltransferase 1 (DNMT1), a dominant enzyme responsible for the transfer of a methyl group from the universal methyl donor to the 5-position of cytosine residues in DNA, is essential for mammalian development and closely related to cancer and a variety of age-related chronic diseases. DNMT1 has become a useful biomarker in early disease diagnosis and a potential therapeutic target in cancer therapy and drug development. However, till now, most of the studies on DNA methyltransferase (MTase) detection have focused on the prokaryote MTase and its activity.Methods: A magnetic fluorescence-linked immunosorbent assay (FLISA) using CdSe/ZnS quantum dots as fluorescent probes was proposed for the rapid and sensitive detection of the DNMT1 level in this study. Key factors that affect the precision and accuracy of the determination of DNMT1 were optimized.Results: Under the optimal conditions, the limit of detection was 0.1 ng/mL, the linear range was 0.1–1,500 ng/mL, the recovery was 91.67%–106.50%, and the relative standard deviations of intra- and inter-assays were respectively 5.45%–11.29% and 7.03%–11.25%. The cross-reactivity rates with DNA methyltransferases 3a and 3b were only 4.0% and 9.4%, respectively. Furthermore, FLISA was successfully used to detect the levels of DNMT1 in human serum samples, and compared with commercial enzyme-linked immunosorbent assay (ELISA) kits. The results revealed that there was a good correlation between FLISA and commercial ELISA kits (correlation coefficient r=0.866, p=0.001). The linear scope of FLISA was broader than ELISA, and the measurement time was much shorter than ELISA kits.Conclusion: These indicated that the proposed FLISA method was sensitive and high throughput and can quickly screen the level of DNMT1 in serum samples. Keywords: DNA methyltransferase 1, quantum dots, fluorescence immunoassay, magnetic carboxyl beads, high throughput, serum sample
format	article
author	Yu F Xiong YM Yu SC He LL Niu SS Wu YM Liu J Qu LB Liu LE Wu YJ
author_facet	Yu F Xiong YM Yu SC He LL Niu SS Wu YM Liu J Qu LB Liu LE Wu YJ
author_sort	Yu F
title	Magnetic immunoassay using CdSe/ZnS quantum dots as fluorescent probes to detect the level of DNA methyltransferase 1 in human serum sample
title_short	Magnetic immunoassay using CdSe/ZnS quantum dots as fluorescent probes to detect the level of DNA methyltransferase 1 in human serum sample
title_full	Magnetic immunoassay using CdSe/ZnS quantum dots as fluorescent probes to detect the level of DNA methyltransferase 1 in human serum sample
title_fullStr	Magnetic immunoassay using CdSe/ZnS quantum dots as fluorescent probes to detect the level of DNA methyltransferase 1 in human serum sample
title_full_unstemmed	Magnetic immunoassay using CdSe/ZnS quantum dots as fluorescent probes to detect the level of DNA methyltransferase 1 in human serum sample
title_sort	magnetic immunoassay using cdse/zns quantum dots as fluorescent probes to detect the level of dna methyltransferase 1 in human serum sample
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/40c60ff662e54254b946edc5c2ed2602
work_keys_str_mv	AT yuf magneticimmunoassayusingcdseznsquantumdotsasfluorescentprobestodetectthelevelofdnamethyltransferase1inhumanserumsample AT xiongym magneticimmunoassayusingcdseznsquantumdotsasfluorescentprobestodetectthelevelofdnamethyltransferase1inhumanserumsample AT yusc magneticimmunoassayusingcdseznsquantumdotsasfluorescentprobestodetectthelevelofdnamethyltransferase1inhumanserumsample AT hell magneticimmunoassayusingcdseznsquantumdotsasfluorescentprobestodetectthelevelofdnamethyltransferase1inhumanserumsample AT niuss magneticimmunoassayusingcdseznsquantumdotsasfluorescentprobestodetectthelevelofdnamethyltransferase1inhumanserumsample AT wuym magneticimmunoassayusingcdseznsquantumdotsasfluorescentprobestodetectthelevelofdnamethyltransferase1inhumanserumsample AT liuj magneticimmunoassayusingcdseznsquantumdotsasfluorescentprobestodetectthelevelofdnamethyltransferase1inhumanserumsample AT qulb magneticimmunoassayusingcdseznsquantumdotsasfluorescentprobestodetectthelevelofdnamethyltransferase1inhumanserumsample AT liule magneticimmunoassayusingcdseznsquantumdotsasfluorescentprobestodetectthelevelofdnamethyltransferase1inhumanserumsample AT wuyj magneticimmunoassayusingcdseznsquantumdotsasfluorescentprobestodetectthelevelofdnamethyltransferase1inhumanserumsample
_version_	1718402644209303552

Magnetic immunoassay using CdSe/ZnS quantum dots as fluorescent probes to detect the level of DNA methyltransferase 1 in human serum sample

Ejemplares similares