Urinary miR-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients.

<h4>Background</h4>Cell-free microRNAs stably and abundantly exist in body fluids and emerging evidence suggests cell-free microRNAs as novel and non-invasive disease biomarker. Deregulation of miR-29 is involved in the pathogenesis of diabetic nephropathy and insulin resistance thus may...

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Autores principales: Hui Peng, Meirong Zhong, Wenbo Zhao, Cheng Wang, Jun Zhang, Xun Liu, Yuanqing Li, Sujay Dutta Paudel, Qianqian Wang, Tanqi Lou
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Publicado: Public Library of Science (PLoS) 2013
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spelling oai:doaj.org-article:40c8ef9ef23a4747a13b34a931f0fb1b2021-11-18T08:42:52ZUrinary miR-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients.1932-620310.1371/journal.pone.0082607https://doaj.org/article/40c8ef9ef23a4747a13b34a931f0fb1b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24349318/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Cell-free microRNAs stably and abundantly exist in body fluids and emerging evidence suggests cell-free microRNAs as novel and non-invasive disease biomarker. Deregulation of miR-29 is involved in the pathogenesis of diabetic nephropathy and insulin resistance thus may be implicated in diabetic vascular complication. Therefore, we investigated the possibility of urinary miR-29 as biomarker for diabetic nephropathy and atherosclerosis in patients with type 2 diabetes.<h4>Methods</h4>83 patients with type 2 diabetes were enrolled in this study, miR-29a, miR-29b and miR-29c levels in urine supernatant was determined by TaqMan qRT-PCR, and a synthetic cel-miR-39 was added to the urine as a spike-in control before miRNAs extraction. Urinary albumin excretion rate and urine albumin/creatinine ratio, funduscopy and carotid ultrasound were used for evaluation of diabetic vascular complication. The laboratory parameters indicating blood glucose level, renal function and serum lipids were also collected.<h4>Results</h4>Patients with albuminuria (n = 42, age 60.62 ± 12.00 yrs) showed significantly higher comorbidity of diabetic retinopathy (p = 0.015) and higher levels of urinary miR-29a (p = 0.035) compared with those with normoalbuminuria (n = 41, age 58.54 ± 14.40 yrs). There was no significant difference in urinary miR-29b (p = 0.148) or miR-29c level (p = 0.321) between groups. Urinary albumin excretion rate significantly correlated with urinary miR-29a level (r = 0.286, p = 0.016), while urinary miR-29b significantly correlated with carotid intima-media thickness (cIMT) (r = 0.286, p = 0.046).<h4>Conclusion</h4>Urinary miR-29a correlated with albuminuria while urinary miR-29b correlated with carotid intima-media thickness (cIMT) in patients with type 2 diabetes. Therefore, they may have the potential to serve as alternative biomarker for diabetic nephropathy and atherosclerosis in type 2 diabetes.Hui PengMeirong ZhongWenbo ZhaoCheng WangJun ZhangXun LiuYuanqing LiSujay Dutta PaudelQianqian WangTanqi LouPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e82607 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Hui Peng
Meirong Zhong
Wenbo Zhao
Cheng Wang
Jun Zhang
Xun Liu
Yuanqing Li
Sujay Dutta Paudel
Qianqian Wang
Tanqi Lou
Urinary miR-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients.
description <h4>Background</h4>Cell-free microRNAs stably and abundantly exist in body fluids and emerging evidence suggests cell-free microRNAs as novel and non-invasive disease biomarker. Deregulation of miR-29 is involved in the pathogenesis of diabetic nephropathy and insulin resistance thus may be implicated in diabetic vascular complication. Therefore, we investigated the possibility of urinary miR-29 as biomarker for diabetic nephropathy and atherosclerosis in patients with type 2 diabetes.<h4>Methods</h4>83 patients with type 2 diabetes were enrolled in this study, miR-29a, miR-29b and miR-29c levels in urine supernatant was determined by TaqMan qRT-PCR, and a synthetic cel-miR-39 was added to the urine as a spike-in control before miRNAs extraction. Urinary albumin excretion rate and urine albumin/creatinine ratio, funduscopy and carotid ultrasound were used for evaluation of diabetic vascular complication. The laboratory parameters indicating blood glucose level, renal function and serum lipids were also collected.<h4>Results</h4>Patients with albuminuria (n = 42, age 60.62 ± 12.00 yrs) showed significantly higher comorbidity of diabetic retinopathy (p = 0.015) and higher levels of urinary miR-29a (p = 0.035) compared with those with normoalbuminuria (n = 41, age 58.54 ± 14.40 yrs). There was no significant difference in urinary miR-29b (p = 0.148) or miR-29c level (p = 0.321) between groups. Urinary albumin excretion rate significantly correlated with urinary miR-29a level (r = 0.286, p = 0.016), while urinary miR-29b significantly correlated with carotid intima-media thickness (cIMT) (r = 0.286, p = 0.046).<h4>Conclusion</h4>Urinary miR-29a correlated with albuminuria while urinary miR-29b correlated with carotid intima-media thickness (cIMT) in patients with type 2 diabetes. Therefore, they may have the potential to serve as alternative biomarker for diabetic nephropathy and atherosclerosis in type 2 diabetes.
format article
author Hui Peng
Meirong Zhong
Wenbo Zhao
Cheng Wang
Jun Zhang
Xun Liu
Yuanqing Li
Sujay Dutta Paudel
Qianqian Wang
Tanqi Lou
author_facet Hui Peng
Meirong Zhong
Wenbo Zhao
Cheng Wang
Jun Zhang
Xun Liu
Yuanqing Li
Sujay Dutta Paudel
Qianqian Wang
Tanqi Lou
author_sort Hui Peng
title Urinary miR-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients.
title_short Urinary miR-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients.
title_full Urinary miR-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients.
title_fullStr Urinary miR-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients.
title_full_unstemmed Urinary miR-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients.
title_sort urinary mir-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients.
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/40c8ef9ef23a4747a13b34a931f0fb1b
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