Urinary miR-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients.

<h4>Background</h4>Cell-free microRNAs stably and abundantly exist in body fluids and emerging evidence suggests cell-free microRNAs as novel and non-invasive disease biomarker. Deregulation of miR-29 is involved in the pathogenesis of diabetic nephropathy and insulin resistance thus may...

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Autores principales:	Hui Peng, Meirong Zhong, Wenbo Zhao, Cheng Wang, Jun Zhang, Xun Liu, Yuanqing Li, Sujay Dutta Paudel, Qianqian Wang, Tanqi Lou
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/40c8ef9ef23a4747a13b34a931f0fb1b
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spelling	oai:doaj.org-article:40c8ef9ef23a4747a13b34a931f0fb1b2021-11-18T08:42:52ZUrinary miR-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients.1932-620310.1371/journal.pone.0082607https://doaj.org/article/40c8ef9ef23a4747a13b34a931f0fb1b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24349318/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Cell-free microRNAs stably and abundantly exist in body fluids and emerging evidence suggests cell-free microRNAs as novel and non-invasive disease biomarker. Deregulation of miR-29 is involved in the pathogenesis of diabetic nephropathy and insulin resistance thus may be implicated in diabetic vascular complication. Therefore, we investigated the possibility of urinary miR-29 as biomarker for diabetic nephropathy and atherosclerosis in patients with type 2 diabetes.<h4>Methods</h4>83 patients with type 2 diabetes were enrolled in this study, miR-29a, miR-29b and miR-29c levels in urine supernatant was determined by TaqMan qRT-PCR, and a synthetic cel-miR-39 was added to the urine as a spike-in control before miRNAs extraction. Urinary albumin excretion rate and urine albumin/creatinine ratio, funduscopy and carotid ultrasound were used for evaluation of diabetic vascular complication. The laboratory parameters indicating blood glucose level, renal function and serum lipids were also collected.<h4>Results</h4>Patients with albuminuria (n = 42, age 60.62 ± 12.00 yrs) showed significantly higher comorbidity of diabetic retinopathy (p = 0.015) and higher levels of urinary miR-29a (p = 0.035) compared with those with normoalbuminuria (n = 41, age 58.54 ± 14.40 yrs). There was no significant difference in urinary miR-29b (p = 0.148) or miR-29c level (p = 0.321) between groups. Urinary albumin excretion rate significantly correlated with urinary miR-29a level (r = 0.286, p = 0.016), while urinary miR-29b significantly correlated with carotid intima-media thickness (cIMT) (r = 0.286, p = 0.046).<h4>Conclusion</h4>Urinary miR-29a correlated with albuminuria while urinary miR-29b correlated with carotid intima-media thickness (cIMT) in patients with type 2 diabetes. Therefore, they may have the potential to serve as alternative biomarker for diabetic nephropathy and atherosclerosis in type 2 diabetes.Hui PengMeirong ZhongWenbo ZhaoCheng WangJun ZhangXun LiuYuanqing LiSujay Dutta PaudelQianqian WangTanqi LouPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e82607 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hui Peng Meirong Zhong Wenbo Zhao Cheng Wang Jun Zhang Xun Liu Yuanqing Li Sujay Dutta Paudel Qianqian Wang Tanqi Lou Urinary miR-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients.
description	<h4>Background</h4>Cell-free microRNAs stably and abundantly exist in body fluids and emerging evidence suggests cell-free microRNAs as novel and non-invasive disease biomarker. Deregulation of miR-29 is involved in the pathogenesis of diabetic nephropathy and insulin resistance thus may be implicated in diabetic vascular complication. Therefore, we investigated the possibility of urinary miR-29 as biomarker for diabetic nephropathy and atherosclerosis in patients with type 2 diabetes.<h4>Methods</h4>83 patients with type 2 diabetes were enrolled in this study, miR-29a, miR-29b and miR-29c levels in urine supernatant was determined by TaqMan qRT-PCR, and a synthetic cel-miR-39 was added to the urine as a spike-in control before miRNAs extraction. Urinary albumin excretion rate and urine albumin/creatinine ratio, funduscopy and carotid ultrasound were used for evaluation of diabetic vascular complication. The laboratory parameters indicating blood glucose level, renal function and serum lipids were also collected.<h4>Results</h4>Patients with albuminuria (n = 42, age 60.62 ± 12.00 yrs) showed significantly higher comorbidity of diabetic retinopathy (p = 0.015) and higher levels of urinary miR-29a (p = 0.035) compared with those with normoalbuminuria (n = 41, age 58.54 ± 14.40 yrs). There was no significant difference in urinary miR-29b (p = 0.148) or miR-29c level (p = 0.321) between groups. Urinary albumin excretion rate significantly correlated with urinary miR-29a level (r = 0.286, p = 0.016), while urinary miR-29b significantly correlated with carotid intima-media thickness (cIMT) (r = 0.286, p = 0.046).<h4>Conclusion</h4>Urinary miR-29a correlated with albuminuria while urinary miR-29b correlated with carotid intima-media thickness (cIMT) in patients with type 2 diabetes. Therefore, they may have the potential to serve as alternative biomarker for diabetic nephropathy and atherosclerosis in type 2 diabetes.
format	article
author	Hui Peng Meirong Zhong Wenbo Zhao Cheng Wang Jun Zhang Xun Liu Yuanqing Li Sujay Dutta Paudel Qianqian Wang Tanqi Lou
author_facet	Hui Peng Meirong Zhong Wenbo Zhao Cheng Wang Jun Zhang Xun Liu Yuanqing Li Sujay Dutta Paudel Qianqian Wang Tanqi Lou
author_sort	Hui Peng
title	Urinary miR-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients.
title_short	Urinary miR-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients.
title_full	Urinary miR-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients.
title_fullStr	Urinary miR-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients.
title_full_unstemmed	Urinary miR-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients.
title_sort	urinary mir-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/40c8ef9ef23a4747a13b34a931f0fb1b
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Urinary miR-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients.

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