SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1

Using DNA affinity chromatography we demonstrate that the S. aureus regulatory proteins MgrA, Rot, SarA, and SarS bind DNA baits derived from the promoter regions associated with the genes encoding aureolysin, ScpAB, SspABC, and SplA-F. Three of four baits also bound SarR and SarZ, the exception in...

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Autores principales:	Aura M. Ramirez, Karen E. Beenken, Stephanie D. Byrum, Alan J. Tackett, Lindsey N. Shaw, Brittney D. Gimza, Mark S. Smeltzer
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	staphylococcus aureus extracellular protease regulation biofilm sara rot mgra sars sarz sarr Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/40cc6068c73e432795025640766a5025
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spelling	oai:doaj.org-article:40cc6068c73e432795025640766a50252021-11-17T14:21:59ZSarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-12150-55942150-560810.1080/21505594.2020.1855923https://doaj.org/article/40cc6068c73e432795025640766a50252020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1855923https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Using DNA affinity chromatography we demonstrate that the S. aureus regulatory proteins MgrA, Rot, SarA, and SarS bind DNA baits derived from the promoter regions associated with the genes encoding aureolysin, ScpAB, SspABC, and SplA-F. Three of four baits also bound SarR and SarZ, the exception in both cases being the ScpAB-associated bait. Using the USA300, methicillin-resistant strain LAC and the USA200, methicillin-sensitive strain UAMS-1, we generated mutations in the genes encoding each of these proteins alone and in combination with sarA and examined the impact on protease production, the accumulation of high molecular weight proteins, and biofilm formation. These studies confirmed that multiple regulatory loci are involved in limiting protease production to a degree that impacts all of these phenotypes, but also demonstrate that sarA plays a predominant role in this regard. Using sarA mutants unable to produce individual proteases alone and in combination with each other, we also demonstrate that the increased production of aureolysin and ScpA is particularly important in defining the biofilm-deficient phenotype of LAC and UAMS-1 sarA mutants, while aureolysin alone plays a key role in defining the reduced accumulation of alpha toxin and overall cytotoxicity as assessed using both osteoblasts and osteoclasts.Aura M. RamirezKaren E. BeenkenStephanie D. ByrumAlan J. TackettLindsey N. ShawBrittney D. GimzaMark S. SmeltzerTaylor & Francis Grouparticlestaphylococcus aureusextracellular proteaseregulationbiofilmsararotmgrasarssarzsarrInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 1738-1762 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	staphylococcus aureus extracellular protease regulation biofilm sara rot mgra sars sarz sarr Infectious and parasitic diseases RC109-216
spellingShingle	staphylococcus aureus extracellular protease regulation biofilm sara rot mgra sars sarz sarr Infectious and parasitic diseases RC109-216 Aura M. Ramirez Karen E. Beenken Stephanie D. Byrum Alan J. Tackett Lindsey N. Shaw Brittney D. Gimza Mark S. Smeltzer SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
description	Using DNA affinity chromatography we demonstrate that the S. aureus regulatory proteins MgrA, Rot, SarA, and SarS bind DNA baits derived from the promoter regions associated with the genes encoding aureolysin, ScpAB, SspABC, and SplA-F. Three of four baits also bound SarR and SarZ, the exception in both cases being the ScpAB-associated bait. Using the USA300, methicillin-resistant strain LAC and the USA200, methicillin-sensitive strain UAMS-1, we generated mutations in the genes encoding each of these proteins alone and in combination with sarA and examined the impact on protease production, the accumulation of high molecular weight proteins, and biofilm formation. These studies confirmed that multiple regulatory loci are involved in limiting protease production to a degree that impacts all of these phenotypes, but also demonstrate that sarA plays a predominant role in this regard. Using sarA mutants unable to produce individual proteases alone and in combination with each other, we also demonstrate that the increased production of aureolysin and ScpA is particularly important in defining the biofilm-deficient phenotype of LAC and UAMS-1 sarA mutants, while aureolysin alone plays a key role in defining the reduced accumulation of alpha toxin and overall cytotoxicity as assessed using both osteoblasts and osteoclasts.
format	article
author	Aura M. Ramirez Karen E. Beenken Stephanie D. Byrum Alan J. Tackett Lindsey N. Shaw Brittney D. Gimza Mark S. Smeltzer
author_facet	Aura M. Ramirez Karen E. Beenken Stephanie D. Byrum Alan J. Tackett Lindsey N. Shaw Brittney D. Gimza Mark S. Smeltzer
author_sort	Aura M. Ramirez
title	SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
title_short	SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
title_full	SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
title_fullStr	SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
title_full_unstemmed	SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
title_sort	sara plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of staphylococcus aureus lac and uams-1
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/40cc6068c73e432795025640766a5025
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SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1

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