Assessment of Corneal Stromal Remodeling and Regeneration after Photorefractive Keratectomy

Assessment of Corneal Stromal Remodeling and Regeneration after Photorefractive Keratectomy

Abstract This study utilizes high resolution multi-dimensional imaging to identify temporal and spatial changes in cell/extracellular matrix (ECM) patterning mediating cell migration, fibrosis, remodeling and regeneration during wound healing. Photorefractive keratectomy (PRK) was performed on rabbi...

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Autores principales: Pouriska B. Kivanany, Kyle C. Grose, Madhavi Tippani, Shan Su, W. Matthew Petroll
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/40d118bf16d9433881159e7adc92e021
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spelling oai:doaj.org-article:40d118bf16d9433881159e7adc92e0212021-12-02T15:09:09ZAssessment of Corneal Stromal Remodeling and Regeneration after Photorefractive Keratectomy10.1038/s41598-018-30372-22045-2322https://doaj.org/article/40d118bf16d9433881159e7adc92e0212018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30372-2https://doaj.org/toc/2045-2322Abstract This study utilizes high resolution multi-dimensional imaging to identify temporal and spatial changes in cell/extracellular matrix (ECM) patterning mediating cell migration, fibrosis, remodeling and regeneration during wound healing. Photorefractive keratectomy (PRK) was performed on rabbits. In some cases, 5([4,6-dichlorotriazin-2yl]-amino)fluorescein (DTAF) was applied immediately after surgery to differentiate native vs. cell-secreted collagen. Corneas were assessed 3–180 days postoperatively using in vivo confocal microscopy, and cell/ECM patterning was evaluated in situ using multiphoton and second harmonic generation (SHG) imaging. 7 days post-PRK, migrating fibroblasts below the ablation site were co-aligned with the stromal lamellae. At day 21, randomly patterned myofibroblasts developed on top of the ablation site; whereas cells underneath were elongated, co-aligned with collagen, and lacked stress fibers. Over time, fibrotic tissue was remodeled into more transparent stromal lamellae. By day 180, stromal thickness was almost completely restored. Stromal regrowth occurred primarily below the ablation interface, and was characterized by co-localization of gaps in DTAF labeling with elongated cells and SHG collagen signaling. Punctate F-actin labeling was detected along cells co-aligned with DTAF and non-DTAF labeled collagen, suggesting cell-ECM interactions. Overall, collagen lamellae appear to provide a template for fibroblast patterning during wound healing that mediates stromal repopulation, regeneration and remodeling.Pouriska B. KivananyKyle C. GroseMadhavi TippaniShan SuW. Matthew PetrollNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Pouriska B. Kivanany
Kyle C. Grose
Madhavi Tippani
Shan Su
W. Matthew Petroll
Assessment of Corneal Stromal Remodeling and Regeneration after Photorefractive Keratectomy
description Abstract This study utilizes high resolution multi-dimensional imaging to identify temporal and spatial changes in cell/extracellular matrix (ECM) patterning mediating cell migration, fibrosis, remodeling and regeneration during wound healing. Photorefractive keratectomy (PRK) was performed on rabbits. In some cases, 5([4,6-dichlorotriazin-2yl]-amino)fluorescein (DTAF) was applied immediately after surgery to differentiate native vs. cell-secreted collagen. Corneas were assessed 3–180 days postoperatively using in vivo confocal microscopy, and cell/ECM patterning was evaluated in situ using multiphoton and second harmonic generation (SHG) imaging. 7 days post-PRK, migrating fibroblasts below the ablation site were co-aligned with the stromal lamellae. At day 21, randomly patterned myofibroblasts developed on top of the ablation site; whereas cells underneath were elongated, co-aligned with collagen, and lacked stress fibers. Over time, fibrotic tissue was remodeled into more transparent stromal lamellae. By day 180, stromal thickness was almost completely restored. Stromal regrowth occurred primarily below the ablation interface, and was characterized by co-localization of gaps in DTAF labeling with elongated cells and SHG collagen signaling. Punctate F-actin labeling was detected along cells co-aligned with DTAF and non-DTAF labeled collagen, suggesting cell-ECM interactions. Overall, collagen lamellae appear to provide a template for fibroblast patterning during wound healing that mediates stromal repopulation, regeneration and remodeling.
format article
author Pouriska B. Kivanany
Kyle C. Grose
Madhavi Tippani
Shan Su
W. Matthew Petroll
author_facet Pouriska B. Kivanany
Kyle C. Grose
Madhavi Tippani
Shan Su
W. Matthew Petroll
author_sort Pouriska B. Kivanany
title Assessment of Corneal Stromal Remodeling and Regeneration after Photorefractive Keratectomy
title_short Assessment of Corneal Stromal Remodeling and Regeneration after Photorefractive Keratectomy
title_full Assessment of Corneal Stromal Remodeling and Regeneration after Photorefractive Keratectomy
title_fullStr Assessment of Corneal Stromal Remodeling and Regeneration after Photorefractive Keratectomy
title_full_unstemmed Assessment of Corneal Stromal Remodeling and Regeneration after Photorefractive Keratectomy
title_sort assessment of corneal stromal remodeling and regeneration after photorefractive keratectomy
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/40d118bf16d9433881159e7adc92e021
work_keys_str_mv AT pouriskabkivanany assessmentofcornealstromalremodelingandregenerationafterphotorefractivekeratectomy
AT kylecgrose assessmentofcornealstromalremodelingandregenerationafterphotorefractivekeratectomy
AT madhavitippani assessmentofcornealstromalremodelingandregenerationafterphotorefractivekeratectomy
AT shansu assessmentofcornealstromalremodelingandregenerationafterphotorefractivekeratectomy
AT wmatthewpetroll assessmentofcornealstromalremodelingandregenerationafterphotorefractivekeratectomy
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