Dynamic Structure of Yeast Septin by Fast Fluctuation-Enhanced Structured Illumination Microscopy

When <i>Saccharomyces cerevisiae</i> divides, a structure composed of different septin proteins arranged according to a certain rule is formed at the cell division site. The structure undergoes multiple remodeling stages during the cell cycle, thus guiding the yeast cells to complete the...

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Autores principales:	Longfang Yao, Li Zhang, Liwen Chen, Xingyu Gong, Jiahui Zhong, Baoju Wang, Yiyan Fei, Lan Mi, Jiong Ma
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	CDC12 septin SRRF SIM super-resolution microscopy living-cell image Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/40d22033fd9a4a9a826ab6d29d45a6a3
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id	oai:doaj.org-article:40d22033fd9a4a9a826ab6d29d45a6a3
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spelling	oai:doaj.org-article:40d22033fd9a4a9a826ab6d29d45a6a32021-11-25T18:24:38ZDynamic Structure of Yeast Septin by Fast Fluctuation-Enhanced Structured Illumination Microscopy10.3390/microorganisms91122552076-2607https://doaj.org/article/40d22033fd9a4a9a826ab6d29d45a6a32021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2255https://doaj.org/toc/2076-2607When <i>Saccharomyces cerevisiae</i> divides, a structure composed of different septin proteins arranged according to a certain rule is formed at the cell division site. The structure undergoes multiple remodeling stages during the cell cycle, thus guiding the yeast cells to complete the entire division process. Although the higher-order structure of septins can be determined using electron microscopy, the septin’s dynamic processes are poorly understood because of limitations in living cell super-resolution imaging technology. Herein, we describe a high lateral resolution and temporal resolution technique, known as fast fluctuation-enhanced structured illumination microscopy (fFE-SIM), which more than doubles the SIM resolution at a frame rate of 38 Hz in living cells. This allows a highly dynamic and sparse septin structure to be observed in <i>Saccharomyces cerevisiae</i>.Longfang YaoLi ZhangLiwen ChenXingyu GongJiahui ZhongBaoju WangYiyan FeiLan MiJiong MaMDPI AGarticleCDC12septinSRRFSIMsuper-resolution microscopyliving-cell imageBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2255, p 2255 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	CDC12 septin SRRF SIM super-resolution microscopy living-cell image Biology (General) QH301-705.5
spellingShingle	CDC12 septin SRRF SIM super-resolution microscopy living-cell image Biology (General) QH301-705.5 Longfang Yao Li Zhang Liwen Chen Xingyu Gong Jiahui Zhong Baoju Wang Yiyan Fei Lan Mi Jiong Ma Dynamic Structure of Yeast Septin by Fast Fluctuation-Enhanced Structured Illumination Microscopy
description	When <i>Saccharomyces cerevisiae</i> divides, a structure composed of different septin proteins arranged according to a certain rule is formed at the cell division site. The structure undergoes multiple remodeling stages during the cell cycle, thus guiding the yeast cells to complete the entire division process. Although the higher-order structure of septins can be determined using electron microscopy, the septin’s dynamic processes are poorly understood because of limitations in living cell super-resolution imaging technology. Herein, we describe a high lateral resolution and temporal resolution technique, known as fast fluctuation-enhanced structured illumination microscopy (fFE-SIM), which more than doubles the SIM resolution at a frame rate of 38 Hz in living cells. This allows a highly dynamic and sparse septin structure to be observed in <i>Saccharomyces cerevisiae</i>.
format	article
author	Longfang Yao Li Zhang Liwen Chen Xingyu Gong Jiahui Zhong Baoju Wang Yiyan Fei Lan Mi Jiong Ma
author_facet	Longfang Yao Li Zhang Liwen Chen Xingyu Gong Jiahui Zhong Baoju Wang Yiyan Fei Lan Mi Jiong Ma
author_sort	Longfang Yao
title	Dynamic Structure of Yeast Septin by Fast Fluctuation-Enhanced Structured Illumination Microscopy
title_short	Dynamic Structure of Yeast Septin by Fast Fluctuation-Enhanced Structured Illumination Microscopy
title_full	Dynamic Structure of Yeast Septin by Fast Fluctuation-Enhanced Structured Illumination Microscopy
title_fullStr	Dynamic Structure of Yeast Septin by Fast Fluctuation-Enhanced Structured Illumination Microscopy
title_full_unstemmed	Dynamic Structure of Yeast Septin by Fast Fluctuation-Enhanced Structured Illumination Microscopy
title_sort	dynamic structure of yeast septin by fast fluctuation-enhanced structured illumination microscopy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/40d22033fd9a4a9a826ab6d29d45a6a3
work_keys_str_mv	AT longfangyao dynamicstructureofyeastseptinbyfastfluctuationenhancedstructuredilluminationmicroscopy AT lizhang dynamicstructureofyeastseptinbyfastfluctuationenhancedstructuredilluminationmicroscopy AT liwenchen dynamicstructureofyeastseptinbyfastfluctuationenhancedstructuredilluminationmicroscopy AT xingyugong dynamicstructureofyeastseptinbyfastfluctuationenhancedstructuredilluminationmicroscopy AT jiahuizhong dynamicstructureofyeastseptinbyfastfluctuationenhancedstructuredilluminationmicroscopy AT baojuwang dynamicstructureofyeastseptinbyfastfluctuationenhancedstructuredilluminationmicroscopy AT yiyanfei dynamicstructureofyeastseptinbyfastfluctuationenhancedstructuredilluminationmicroscopy AT lanmi dynamicstructureofyeastseptinbyfastfluctuationenhancedstructuredilluminationmicroscopy AT jiongma dynamicstructureofyeastseptinbyfastfluctuationenhancedstructuredilluminationmicroscopy
_version_	1718411189899231232

Dynamic Structure of Yeast Septin by Fast Fluctuation-Enhanced Structured Illumination Microscopy

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