Three-dimensional and two-dimensional relationships of gangliogenesis with folliculogenesis in mature mouse ovary: a Golgi–Cox staining approach

Abstract The present study was set out to investigate two-dimensional (2D) and three-dimensional (3D) evaluations of ovarian nervous network development and the structural relationship between folliculogenesis and gangliogenesis in mouse ovaries. Adult mice ovarian tissue samples were collected from...

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Autores principales:	Mohammad Ebrahim Asadi Zarch, Alireza Afshar, Farhad Rahmanifar, Mohammad Reza Jafarzadeh Shirazi, Mandana Baghban, Mohammad Dadpasand, Farzad Mohammad Rezazadeh, Arezoo Khoradmehr, Hossein Baharvand, Amin Tamadon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/40d77a9f1bd641a09d77081f546e4c67
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spelling	oai:doaj.org-article:40d77a9f1bd641a09d77081f546e4c672021-12-02T15:52:59ZThree-dimensional and two-dimensional relationships of gangliogenesis with folliculogenesis in mature mouse ovary: a Golgi–Cox staining approach10.1038/s41598-021-84835-02045-2322https://doaj.org/article/40d77a9f1bd641a09d77081f546e4c672021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84835-0https://doaj.org/toc/2045-2322Abstract The present study was set out to investigate two-dimensional (2D) and three-dimensional (3D) evaluations of ovarian nervous network development and the structural relationship between folliculogenesis and gangliogenesis in mouse ovaries. Adult mice ovarian tissue samples were collected from follicular and luteal phases after cardiac perfusion. Ovarian samples were stained by a Golgi–Cox protocol. Following staining, tissues were serially sectioned for imaging. Neural filaments and ganglia were present in the ovaries. In both 2D and 3D studies, an increase in the number and area of ganglia was seen during the follicular growth. The same pattern was also seen in corpora lutea development. However, in some cases such as ratio of ganglia number to follicle area, the ratio of ganglia area to follicular area, 2D findings were different compared with the 3D results. 3D analysis of ovarian gangliogenesis showed the possible direct effect of them on folliculogenesis. Golgi–Cox staining was used in this study for 3D evaluation in non-brain tissue. The results of 3D analysis of the present study showed that, in some cases, the information provided by 2D analysis does not match the reality of ovarian neuronal function. This confirmed the importance of 3D analysis for evaluation of ovarian function.Mohammad Ebrahim Asadi ZarchAlireza AfsharFarhad RahmanifarMohammad Reza Jafarzadeh ShiraziMandana BaghbanMohammad DadpasandFarzad Mohammad RezazadehArezoo KhoradmehrHossein BaharvandAmin TamadonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mohammad Ebrahim Asadi Zarch Alireza Afshar Farhad Rahmanifar Mohammad Reza Jafarzadeh Shirazi Mandana Baghban Mohammad Dadpasand Farzad Mohammad Rezazadeh Arezoo Khoradmehr Hossein Baharvand Amin Tamadon Three-dimensional and two-dimensional relationships of gangliogenesis with folliculogenesis in mature mouse ovary: a Golgi–Cox staining approach
description	Abstract The present study was set out to investigate two-dimensional (2D) and three-dimensional (3D) evaluations of ovarian nervous network development and the structural relationship between folliculogenesis and gangliogenesis in mouse ovaries. Adult mice ovarian tissue samples were collected from follicular and luteal phases after cardiac perfusion. Ovarian samples were stained by a Golgi–Cox protocol. Following staining, tissues were serially sectioned for imaging. Neural filaments and ganglia were present in the ovaries. In both 2D and 3D studies, an increase in the number and area of ganglia was seen during the follicular growth. The same pattern was also seen in corpora lutea development. However, in some cases such as ratio of ganglia number to follicle area, the ratio of ganglia area to follicular area, 2D findings were different compared with the 3D results. 3D analysis of ovarian gangliogenesis showed the possible direct effect of them on folliculogenesis. Golgi–Cox staining was used in this study for 3D evaluation in non-brain tissue. The results of 3D analysis of the present study showed that, in some cases, the information provided by 2D analysis does not match the reality of ovarian neuronal function. This confirmed the importance of 3D analysis for evaluation of ovarian function.
format	article
author	Mohammad Ebrahim Asadi Zarch Alireza Afshar Farhad Rahmanifar Mohammad Reza Jafarzadeh Shirazi Mandana Baghban Mohammad Dadpasand Farzad Mohammad Rezazadeh Arezoo Khoradmehr Hossein Baharvand Amin Tamadon
author_facet	Mohammad Ebrahim Asadi Zarch Alireza Afshar Farhad Rahmanifar Mohammad Reza Jafarzadeh Shirazi Mandana Baghban Mohammad Dadpasand Farzad Mohammad Rezazadeh Arezoo Khoradmehr Hossein Baharvand Amin Tamadon
author_sort	Mohammad Ebrahim Asadi Zarch
title	Three-dimensional and two-dimensional relationships of gangliogenesis with folliculogenesis in mature mouse ovary: a Golgi–Cox staining approach
title_short	Three-dimensional and two-dimensional relationships of gangliogenesis with folliculogenesis in mature mouse ovary: a Golgi–Cox staining approach
title_full	Three-dimensional and two-dimensional relationships of gangliogenesis with folliculogenesis in mature mouse ovary: a Golgi–Cox staining approach
title_fullStr	Three-dimensional and two-dimensional relationships of gangliogenesis with folliculogenesis in mature mouse ovary: a Golgi–Cox staining approach
title_full_unstemmed	Three-dimensional and two-dimensional relationships of gangliogenesis with folliculogenesis in mature mouse ovary: a Golgi–Cox staining approach
title_sort	three-dimensional and two-dimensional relationships of gangliogenesis with folliculogenesis in mature mouse ovary: a golgi–cox staining approach
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/40d77a9f1bd641a09d77081f546e4c67
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Three-dimensional and two-dimensional relationships of gangliogenesis with folliculogenesis in mature mouse ovary: a Golgi–Cox staining approach

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