Genetic Analysis of the IncX4 Plasmids: Implications for a Unique Pattern in the mcr-1 Acquisition

Abstract IncX4 plasmids are associated with the dissemination of the mcr-1 genes in Enterobacteriaceae. We screened IncX4 plasmids among 2,470 isolates of Enterobacteriaceae and determined the mcr-1 positive isolates. Forty-three isolates were observed to carry IncX4 type plasmid, among which 13 wer...

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Autores principales:	Jian Sun, Liang-Xing Fang, Zuowei Wu, Hui Deng, Run-Shi Yang, Xing-Ping Li, Shu-Min Li, Xiao-Ping Liao, Youjun Feng, Ya-Hong Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/40e2d3233c0c47219969b5688a1a7587
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spelling	oai:doaj.org-article:40e2d3233c0c47219969b5688a1a75872021-12-02T12:32:15ZGenetic Analysis of the IncX4 Plasmids: Implications for a Unique Pattern in the mcr-1 Acquisition10.1038/s41598-017-00095-x2045-2322https://doaj.org/article/40e2d3233c0c47219969b5688a1a75872017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00095-xhttps://doaj.org/toc/2045-2322Abstract IncX4 plasmids are associated with the dissemination of the mcr-1 genes in Enterobacteriaceae. We screened IncX4 plasmids among 2,470 isolates of Enterobacteriaceae and determined the mcr-1 positive isolates. Forty-three isolates were observed to carry IncX4 type plasmid, among which 13 were identified to carry mcr-1 gene. Three representative mcr-1-positive IncX4 plasmids were selected for high-throughput sequencing. Comparative genomics showed that the mcr-1-carrying IncX4 plasmids exhibit remarkable similarity in the backbone, and the major distinction lies in the region containing mcr-1. The major variable regions of all the IncX4 plasmids were fully characterized by PCR-RFLP. The results revealed that the mcr-1 was located on the Variable Region I of IncX4 plasmids in 11 E. coli isolates. Among them, nine E. coli strains possess an epidemic pCSZ4-like IncX4 plasmid containing mcr-1. ISApl1 was presumably involved in the transposition of the mcr-1 cassette and then was lost. Similar genetic contexts were found in different plasmids, even the E. coli chromosome, implying the acquisition of mcr-1 by a unique common mechanism.Jian SunLiang-Xing FangZuowei WuHui DengRun-Shi YangXing-Ping LiShu-Min LiXiao-Ping LiaoYoujun FengYa-Hong LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jian Sun Liang-Xing Fang Zuowei Wu Hui Deng Run-Shi Yang Xing-Ping Li Shu-Min Li Xiao-Ping Liao Youjun Feng Ya-Hong Liu Genetic Analysis of the IncX4 Plasmids: Implications for a Unique Pattern in the mcr-1 Acquisition
description	Abstract IncX4 plasmids are associated with the dissemination of the mcr-1 genes in Enterobacteriaceae. We screened IncX4 plasmids among 2,470 isolates of Enterobacteriaceae and determined the mcr-1 positive isolates. Forty-three isolates were observed to carry IncX4 type plasmid, among which 13 were identified to carry mcr-1 gene. Three representative mcr-1-positive IncX4 plasmids were selected for high-throughput sequencing. Comparative genomics showed that the mcr-1-carrying IncX4 plasmids exhibit remarkable similarity in the backbone, and the major distinction lies in the region containing mcr-1. The major variable regions of all the IncX4 plasmids were fully characterized by PCR-RFLP. The results revealed that the mcr-1 was located on the Variable Region I of IncX4 plasmids in 11 E. coli isolates. Among them, nine E. coli strains possess an epidemic pCSZ4-like IncX4 plasmid containing mcr-1. ISApl1 was presumably involved in the transposition of the mcr-1 cassette and then was lost. Similar genetic contexts were found in different plasmids, even the E. coli chromosome, implying the acquisition of mcr-1 by a unique common mechanism.
format	article
author	Jian Sun Liang-Xing Fang Zuowei Wu Hui Deng Run-Shi Yang Xing-Ping Li Shu-Min Li Xiao-Ping Liao Youjun Feng Ya-Hong Liu
author_facet	Jian Sun Liang-Xing Fang Zuowei Wu Hui Deng Run-Shi Yang Xing-Ping Li Shu-Min Li Xiao-Ping Liao Youjun Feng Ya-Hong Liu
author_sort	Jian Sun
title	Genetic Analysis of the IncX4 Plasmids: Implications for a Unique Pattern in the mcr-1 Acquisition
title_short	Genetic Analysis of the IncX4 Plasmids: Implications for a Unique Pattern in the mcr-1 Acquisition
title_full	Genetic Analysis of the IncX4 Plasmids: Implications for a Unique Pattern in the mcr-1 Acquisition
title_fullStr	Genetic Analysis of the IncX4 Plasmids: Implications for a Unique Pattern in the mcr-1 Acquisition
title_full_unstemmed	Genetic Analysis of the IncX4 Plasmids: Implications for a Unique Pattern in the mcr-1 Acquisition
title_sort	genetic analysis of the incx4 plasmids: implications for a unique pattern in the mcr-1 acquisition
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/40e2d3233c0c47219969b5688a1a7587
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Genetic Analysis of the IncX4 Plasmids: Implications for a Unique Pattern in the mcr-1 Acquisition

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