COMPARISON OF INFLUENZA A(H1N1)pdm09 GENOMES OBTAINED FROM DIFFERENT TYPES OF VIRUS-CONTAINING MATERIAL

This paper describes results of comparison of genetic sequences of inf luenza viruses obtained from clinical samples (nasopharyngeal swabs) and viruses isolated on MDCK cells and in developing chick embryos. The obtained data shows that 1–2x fold passaging of inf luenza viruses in MDCK cells does no...

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Autores principales:	A. V. Fadeev, I. N. Zhilinskaya
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2017
Materias:	influenza a virus whole-genome sequencing epidemiological surveillance hemagglutinin adaptation mutations receptor specificity Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/40ec56f06550457cbf850509dd345fd9
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spelling	oai:doaj.org-article:40ec56f06550457cbf850509dd345fd92021-11-22T07:09:48ZCOMPARISON OF INFLUENZA A(H1N1)pdm09 GENOMES OBTAINED FROM DIFFERENT TYPES OF VIRUS-CONTAINING MATERIAL2220-76192313-739810.15789/2220-7619-2017-3-292-296https://doaj.org/article/40ec56f06550457cbf850509dd345fd92017-09-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/562https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398This paper describes results of comparison of genetic sequences of inf luenza viruses obtained from clinical samples (nasopharyngeal swabs) and viruses isolated on MDCK cells and in developing chick embryos. The obtained data shows that 1–2x fold passaging of inf luenza viruses in MDCK cells does not lead to additional mutations in the genome of the inf luenza virus, and the genetic material of this virus is identical to the genetic material from the clinical sample. When passaging viruses in chick embryos within 1–2 passages, the genetic material of the virus differs from the genetic material from the clinical sample, because of mutations in the receptor region of hemagglutinin, leading to aminoacid substitution Q223R. The mutation Q223R changes the receptor specificity of hemagglutinin from human-type receptors (α-2.6-sialic acids) to avian receptors (α-2.3-sialic acids). The data obtained coincide with the data of Japanese researchers who showed that a similar substitution in the hemagglutinin gene was detected in Japanese strains of inf luenza virus subtype A (H1N1) pdm09, passaged in chick embryos for 1–2 passages. With their further passaging in chick embryos, the number of acquired mutations in the surface proteins of these viruses increases. Thus, we have shown that for the genetic analysis of epidemic-relevant strains of inf luenza viruses for routine epidemiological surveillance tasks, the use of direct sequencing of clinical samples containing the genetic material of viruses makes it possible to obtain the most reliable data, to increase their efficiency, since it saves time normally spent on virus isolation procedure. If preliminary isolation of viruses is necessary, for example, if it is not possible to carry out full genome amplification from a clinical sample (low content of the material, presence of polymerase chain reaction inhibitors), virus isolation on MDCK cells with a minimal amount of passage is optimal, which is used in practice of epidemiological surveillance.A. V. FadeevI. N. ZhilinskayaSankt-Peterburg : NIIÈM imeni Pasteraarticleinfluenza a viruswhole-genome sequencingepidemiological surveillancehemagglutininadaptation mutationsreceptor specificityInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 7, Iss 3, Pp 292-296 (2017)
institution	DOAJ
collection	DOAJ
language	RU
topic	influenza a virus whole-genome sequencing epidemiological surveillance hemagglutinin adaptation mutations receptor specificity Infectious and parasitic diseases RC109-216
spellingShingle	influenza a virus whole-genome sequencing epidemiological surveillance hemagglutinin adaptation mutations receptor specificity Infectious and parasitic diseases RC109-216 A. V. Fadeev I. N. Zhilinskaya COMPARISON OF INFLUENZA A(H1N1)pdm09 GENOMES OBTAINED FROM DIFFERENT TYPES OF VIRUS-CONTAINING MATERIAL
description	This paper describes results of comparison of genetic sequences of inf luenza viruses obtained from clinical samples (nasopharyngeal swabs) and viruses isolated on MDCK cells and in developing chick embryos. The obtained data shows that 1–2x fold passaging of inf luenza viruses in MDCK cells does not lead to additional mutations in the genome of the inf luenza virus, and the genetic material of this virus is identical to the genetic material from the clinical sample. When passaging viruses in chick embryos within 1–2 passages, the genetic material of the virus differs from the genetic material from the clinical sample, because of mutations in the receptor region of hemagglutinin, leading to aminoacid substitution Q223R. The mutation Q223R changes the receptor specificity of hemagglutinin from human-type receptors (α-2.6-sialic acids) to avian receptors (α-2.3-sialic acids). The data obtained coincide with the data of Japanese researchers who showed that a similar substitution in the hemagglutinin gene was detected in Japanese strains of inf luenza virus subtype A (H1N1) pdm09, passaged in chick embryos for 1–2 passages. With their further passaging in chick embryos, the number of acquired mutations in the surface proteins of these viruses increases. Thus, we have shown that for the genetic analysis of epidemic-relevant strains of inf luenza viruses for routine epidemiological surveillance tasks, the use of direct sequencing of clinical samples containing the genetic material of viruses makes it possible to obtain the most reliable data, to increase their efficiency, since it saves time normally spent on virus isolation procedure. If preliminary isolation of viruses is necessary, for example, if it is not possible to carry out full genome amplification from a clinical sample (low content of the material, presence of polymerase chain reaction inhibitors), virus isolation on MDCK cells with a minimal amount of passage is optimal, which is used in practice of epidemiological surveillance.
format	article
author	A. V. Fadeev I. N. Zhilinskaya
author_facet	A. V. Fadeev I. N. Zhilinskaya
author_sort	A. V. Fadeev
title	COMPARISON OF INFLUENZA A(H1N1)pdm09 GENOMES OBTAINED FROM DIFFERENT TYPES OF VIRUS-CONTAINING MATERIAL
title_short	COMPARISON OF INFLUENZA A(H1N1)pdm09 GENOMES OBTAINED FROM DIFFERENT TYPES OF VIRUS-CONTAINING MATERIAL
title_full	COMPARISON OF INFLUENZA A(H1N1)pdm09 GENOMES OBTAINED FROM DIFFERENT TYPES OF VIRUS-CONTAINING MATERIAL
title_fullStr	COMPARISON OF INFLUENZA A(H1N1)pdm09 GENOMES OBTAINED FROM DIFFERENT TYPES OF VIRUS-CONTAINING MATERIAL
title_full_unstemmed	COMPARISON OF INFLUENZA A(H1N1)pdm09 GENOMES OBTAINED FROM DIFFERENT TYPES OF VIRUS-CONTAINING MATERIAL
title_sort	comparison of influenza a(h1n1)pdm09 genomes obtained from different types of virus-containing material
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2017
url	https://doaj.org/article/40ec56f06550457cbf850509dd345fd9
work_keys_str_mv	AT avfadeev comparisonofinfluenzaah1n1pdm09genomesobtainedfromdifferenttypesofviruscontainingmaterial AT inzhilinskaya comparisonofinfluenzaah1n1pdm09genomesobtainedfromdifferenttypesofviruscontainingmaterial
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COMPARISON OF INFLUENZA A(H1N1)pdm09 GENOMES OBTAINED FROM DIFFERENT TYPES OF VIRUS-CONTAINING MATERIAL

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