Synergy between “Probiotic” Carbon Quantum Dots and Ciprofloxacin in Eradicating Infectious Biofilms and Their Biosafety in Mice

Orally administrated probiotic bacteria can aid antibiotic treatment of intestinal infections, but their arrival at their intestinal target site is hampered by killing in the gastrointestinal tract and by antibiotics solely intended for pathogen killing. Carbon-quantum-dots are extremely small nanop...

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Autores principales:	Yanyan Wu, Guang Yang, Henny C. van der Mei, Linqi Shi, Henk J. Busscher, Yijin Ren
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	matrix disruption antibiotics <i>Bifidobacterium breve</i> <i>Escherichia coli</i> reactive oxygen species intestinal infections Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/40ed58d8d56244d788ff46ea78c004c5
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spelling	oai:doaj.org-article:40ed58d8d56244d788ff46ea78c004c52021-11-25T18:40:51ZSynergy between “Probiotic” Carbon Quantum Dots and Ciprofloxacin in Eradicating Infectious Biofilms and Their Biosafety in Mice10.3390/pharmaceutics131118091999-4923https://doaj.org/article/40ed58d8d56244d788ff46ea78c004c52021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1809https://doaj.org/toc/1999-4923Orally administrated probiotic bacteria can aid antibiotic treatment of intestinal infections, but their arrival at their intestinal target site is hampered by killing in the gastrointestinal tract and by antibiotics solely intended for pathogen killing. Carbon-quantum-dots are extremely small nanoparticles and can be derived from different sources, including bacteria. Here, we hypothesize that carbon-quantum-dots inherit antibacterial activity from probiotic source bacteria to fulfill a similar role as live probiotics in intestinal infection therapy. Physico-chemical analyses indicated that carbon-quantum-dots, hydrothermally derived from <i>Bifidobacterium breve</i> (B-C-dots), inherited proteins and polysaccharides from their source-bacteria. B-C-dots disrupted biofilm matrices of <i>Escherichia coli</i> and <i>Salmonella typhimurium</i> biofilms through extensive reactive-oxygen-species (ROS)-generation, causing a decrease in volumetric bacterial-density in biofilms. Decreased bacterial densities leave more open space in biofilms and have enhanced ciprofloxacin penetration and killing potential in an <i>E. coli</i> biofilm pre-exposed to probiotic B-C-dots. Pathogenic carbon-quantum-dots hydrothermally derived from <i>E. coli</i> (E-C-dots) did not disrupt pathogenic biofilms nor enhance <i>E. coli</i> killing potential by ciprofloxacin. B-C-dots were biosafe in mice upon daily administration, while E-C-dots demonstrated a decrease in white blood cell and platelet counts and an increase in C-reactive protein levels. Therefore, the way is paved for employing probiotic carbon-quantum-dots instead of viable, probiotic bacteria for synergistic use with existing antibiotics in treating intestinal infections.Yanyan WuGuang YangHenny C. van der MeiLinqi ShiHenk J. BusscherYijin RenMDPI AGarticlematrix disruptionantibiotics<i>Bifidobacterium breve</i><i>Escherichia coli</i>reactive oxygen speciesintestinal infectionsPharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1809, p 1809 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	matrix disruption antibiotics <i>Bifidobacterium breve</i> <i>Escherichia coli</i> reactive oxygen species intestinal infections Pharmacy and materia medica RS1-441
spellingShingle	matrix disruption antibiotics <i>Bifidobacterium breve</i> <i>Escherichia coli</i> reactive oxygen species intestinal infections Pharmacy and materia medica RS1-441 Yanyan Wu Guang Yang Henny C. van der Mei Linqi Shi Henk J. Busscher Yijin Ren Synergy between “Probiotic” Carbon Quantum Dots and Ciprofloxacin in Eradicating Infectious Biofilms and Their Biosafety in Mice
description	Orally administrated probiotic bacteria can aid antibiotic treatment of intestinal infections, but their arrival at their intestinal target site is hampered by killing in the gastrointestinal tract and by antibiotics solely intended for pathogen killing. Carbon-quantum-dots are extremely small nanoparticles and can be derived from different sources, including bacteria. Here, we hypothesize that carbon-quantum-dots inherit antibacterial activity from probiotic source bacteria to fulfill a similar role as live probiotics in intestinal infection therapy. Physico-chemical analyses indicated that carbon-quantum-dots, hydrothermally derived from <i>Bifidobacterium breve</i> (B-C-dots), inherited proteins and polysaccharides from their source-bacteria. B-C-dots disrupted biofilm matrices of <i>Escherichia coli</i> and <i>Salmonella typhimurium</i> biofilms through extensive reactive-oxygen-species (ROS)-generation, causing a decrease in volumetric bacterial-density in biofilms. Decreased bacterial densities leave more open space in biofilms and have enhanced ciprofloxacin penetration and killing potential in an <i>E. coli</i> biofilm pre-exposed to probiotic B-C-dots. Pathogenic carbon-quantum-dots hydrothermally derived from <i>E. coli</i> (E-C-dots) did not disrupt pathogenic biofilms nor enhance <i>E. coli</i> killing potential by ciprofloxacin. B-C-dots were biosafe in mice upon daily administration, while E-C-dots demonstrated a decrease in white blood cell and platelet counts and an increase in C-reactive protein levels. Therefore, the way is paved for employing probiotic carbon-quantum-dots instead of viable, probiotic bacteria for synergistic use with existing antibiotics in treating intestinal infections.
format	article
author	Yanyan Wu Guang Yang Henny C. van der Mei Linqi Shi Henk J. Busscher Yijin Ren
author_facet	Yanyan Wu Guang Yang Henny C. van der Mei Linqi Shi Henk J. Busscher Yijin Ren
author_sort	Yanyan Wu
title	Synergy between “Probiotic” Carbon Quantum Dots and Ciprofloxacin in Eradicating Infectious Biofilms and Their Biosafety in Mice
title_short	Synergy between “Probiotic” Carbon Quantum Dots and Ciprofloxacin in Eradicating Infectious Biofilms and Their Biosafety in Mice
title_full	Synergy between “Probiotic” Carbon Quantum Dots and Ciprofloxacin in Eradicating Infectious Biofilms and Their Biosafety in Mice
title_fullStr	Synergy between “Probiotic” Carbon Quantum Dots and Ciprofloxacin in Eradicating Infectious Biofilms and Their Biosafety in Mice
title_full_unstemmed	Synergy between “Probiotic” Carbon Quantum Dots and Ciprofloxacin in Eradicating Infectious Biofilms and Their Biosafety in Mice
title_sort	synergy between “probiotic” carbon quantum dots and ciprofloxacin in eradicating infectious biofilms and their biosafety in mice
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/40ed58d8d56244d788ff46ea78c004c5
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_version_	1718410828531630080

Synergy between “Probiotic” Carbon Quantum Dots and Ciprofloxacin in Eradicating Infectious Biofilms and Their Biosafety in Mice

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