The effect of gamma rays irradiation on diversity of Musa paradisiaca var. sapientum as revealed by ISSR molecular marker

Abstract. Due MS, Susilowati A, Yunus A. 2019. The effect of gamma rays irradiation on diversity of Musa paradisiaca var. sapientum as revealed by ISSR molecular marker. Biodiversitas 20: 1416-1422. Pisang Ambon (Musa paradisiaca var. sapientum (L.) Kunt.) is one of the horticultural commodities tha...

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Autores principales:	MARIA SERVIANA DUE, ARI SUSILOWATI, AHMAD YUNUS
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	diversity, gamma ray, irradiation, molecular markers, pisang ambon Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/40f0d9afc5df4318a120837dc0422cba
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spelling	oai:doaj.org-article:40f0d9afc5df4318a120837dc0422cba2021-11-21T21:40:43ZThe effect of gamma rays irradiation on diversity of Musa paradisiaca var. sapientum as revealed by ISSR molecular marker1412-033X2085-472210.13057/biodiv/d200534https://doaj.org/article/40f0d9afc5df4318a120837dc0422cba2019-04-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3508https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Due MS, Susilowati A, Yunus A. 2019. The effect of gamma rays irradiation on diversity of Musa paradisiaca var. sapientum as revealed by ISSR molecular marker. Biodiversitas 20: 1416-1422. Pisang Ambon (Musa paradisiaca var. sapientum (L.) Kunt.) is one of the horticultural commodities that have high production in Indonesia, making it a superior product. Bananas are propagated vegetatively, which hampers the production of genetic variation and requires a long generation time in the vegetative cycle. This study aimed to determine the diversity of Pisang Ambon induced by gamma radiation of various dosages (10 Gy, 20 Gy and 30 Gy) in vitro based on the ISSR (Inter-Simple Sequence Repeats) marker. The results showed that gamma-ray radiation influenced the morphological properties of plant height, stem diameter, leaf length, leaf width and leaf number based on ANOVA variance analysis. Increased growth occurs in plants with radiation doses of 10 Gy and 20 Gy. The molecular analysis of ISSR uses 6 primers, namely ISSR 835, ISSR 836, ISSR 846, ISSR 847, ISSR 848 and ISSR 855. Of the 6 primers, only 2 primers were able to produce polymorphisms above 50%. A total of 9 bands were produced, in which 8 bands showed polymorphic with a percentage of 90%. The similarity matrix ranged from 0.25-0.93, indicating the level of variation between mutants. The result of dendrogram was divided into two groups with a coefficient distance of 0.47-0.93; plants at a dose of 10 Gy have the highest diversity with the furthest coefficient of diversity distance of 0.47.MARIA SERVIANA DUEARI SUSILOWATIAHMAD YUNUSMBI & UNS Soloarticlediversity, gamma ray, irradiation, molecular markers, pisang ambonBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 5 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	diversity, gamma ray, irradiation, molecular markers, pisang ambon Biology (General) QH301-705.5
spellingShingle	diversity, gamma ray, irradiation, molecular markers, pisang ambon Biology (General) QH301-705.5 MARIA SERVIANA DUE ARI SUSILOWATI AHMAD YUNUS The effect of gamma rays irradiation on diversity of Musa paradisiaca var. sapientum as revealed by ISSR molecular marker
description	Abstract. Due MS, Susilowati A, Yunus A. 2019. The effect of gamma rays irradiation on diversity of Musa paradisiaca var. sapientum as revealed by ISSR molecular marker. Biodiversitas 20: 1416-1422. Pisang Ambon (Musa paradisiaca var. sapientum (L.) Kunt.) is one of the horticultural commodities that have high production in Indonesia, making it a superior product. Bananas are propagated vegetatively, which hampers the production of genetic variation and requires a long generation time in the vegetative cycle. This study aimed to determine the diversity of Pisang Ambon induced by gamma radiation of various dosages (10 Gy, 20 Gy and 30 Gy) in vitro based on the ISSR (Inter-Simple Sequence Repeats) marker. The results showed that gamma-ray radiation influenced the morphological properties of plant height, stem diameter, leaf length, leaf width and leaf number based on ANOVA variance analysis. Increased growth occurs in plants with radiation doses of 10 Gy and 20 Gy. The molecular analysis of ISSR uses 6 primers, namely ISSR 835, ISSR 836, ISSR 846, ISSR 847, ISSR 848 and ISSR 855. Of the 6 primers, only 2 primers were able to produce polymorphisms above 50%. A total of 9 bands were produced, in which 8 bands showed polymorphic with a percentage of 90%. The similarity matrix ranged from 0.25-0.93, indicating the level of variation between mutants. The result of dendrogram was divided into two groups with a coefficient distance of 0.47-0.93; plants at a dose of 10 Gy have the highest diversity with the furthest coefficient of diversity distance of 0.47.
format	article
author	MARIA SERVIANA DUE ARI SUSILOWATI AHMAD YUNUS
author_facet	MARIA SERVIANA DUE ARI SUSILOWATI AHMAD YUNUS
author_sort	MARIA SERVIANA DUE
title	The effect of gamma rays irradiation on diversity of Musa paradisiaca var. sapientum as revealed by ISSR molecular marker
title_short	The effect of gamma rays irradiation on diversity of Musa paradisiaca var. sapientum as revealed by ISSR molecular marker
title_full	The effect of gamma rays irradiation on diversity of Musa paradisiaca var. sapientum as revealed by ISSR molecular marker
title_fullStr	The effect of gamma rays irradiation on diversity of Musa paradisiaca var. sapientum as revealed by ISSR molecular marker
title_full_unstemmed	The effect of gamma rays irradiation on diversity of Musa paradisiaca var. sapientum as revealed by ISSR molecular marker
title_sort	effect of gamma rays irradiation on diversity of musa paradisiaca var. sapientum as revealed by issr molecular marker
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/40f0d9afc5df4318a120837dc0422cba
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The effect of gamma rays irradiation on diversity of Musa paradisiaca var. sapientum as revealed by ISSR molecular marker

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