Three-dimensional ultrastructural analysis of the interface between an implanted demineralised dentin matrix and the surrounding newly formed bone

Abstract Previous investigators have reported that transplanted demineralised dentin matrix (DDM) influences bone formation in vivo. However, the specific mechanism of how dentinal tubules contribute to bone formation has not been determined with regard to DDM transplantation therapy. In this study,...

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Autores principales:	Ryuichiro Tanoue, Keisuke Ohta, Yoshihiro Miyazono, Joe Iwanaga, Akihiro Koba, Toru Natori, Osamu Iwamoto, Kei-ichiro Nakamura, Jingo Kusukawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/40f6e0f760a54fadbb98a9f768296e02
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spelling	oai:doaj.org-article:40f6e0f760a54fadbb98a9f768296e022021-12-02T15:09:02ZThree-dimensional ultrastructural analysis of the interface between an implanted demineralised dentin matrix and the surrounding newly formed bone10.1038/s41598-018-21291-32045-2322https://doaj.org/article/40f6e0f760a54fadbb98a9f768296e022018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21291-3https://doaj.org/toc/2045-2322Abstract Previous investigators have reported that transplanted demineralised dentin matrix (DDM) influences bone formation in vivo. However, the specific mechanism of how dentinal tubules contribute to bone formation has not been determined with regard to DDM transplantation therapy. In this study, we ultrastructurally investigated how DDM contacted the surrounding newly formed bone using a scanning electron microscopy (SEM) three-dimensional reconstruction method that is based on focused ion beam slicing and SEM (FIB/SEM). A pulverised and processed DDM derived from human teeth was implanted into rat calvarial bone defects, and a series of X-ray computed tomographic images were obtained over 12 weeks. Implants with surrounding new bone were removed and histologically examined using FIB/SEM. After obtaining objective block-face images, the target boundary face was reconstructed three-dimensionally. The osteocytes of the new bone tissue surrounding the DDM formed a network connected by their cellular processes and formed bone tissue. It is also interesting that the cellular processes of the osteocytes extended into the dentinal tubules, and that bone tissue with canaliculi had formed and filled the DDM surface.Ryuichiro TanoueKeisuke OhtaYoshihiro MiyazonoJoe IwanagaAkihiro KobaToru NatoriOsamu IwamotoKei-ichiro NakamuraJingo KusukawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ryuichiro Tanoue Keisuke Ohta Yoshihiro Miyazono Joe Iwanaga Akihiro Koba Toru Natori Osamu Iwamoto Kei-ichiro Nakamura Jingo Kusukawa Three-dimensional ultrastructural analysis of the interface between an implanted demineralised dentin matrix and the surrounding newly formed bone
description	Abstract Previous investigators have reported that transplanted demineralised dentin matrix (DDM) influences bone formation in vivo. However, the specific mechanism of how dentinal tubules contribute to bone formation has not been determined with regard to DDM transplantation therapy. In this study, we ultrastructurally investigated how DDM contacted the surrounding newly formed bone using a scanning electron microscopy (SEM) three-dimensional reconstruction method that is based on focused ion beam slicing and SEM (FIB/SEM). A pulverised and processed DDM derived from human teeth was implanted into rat calvarial bone defects, and a series of X-ray computed tomographic images were obtained over 12 weeks. Implants with surrounding new bone were removed and histologically examined using FIB/SEM. After obtaining objective block-face images, the target boundary face was reconstructed three-dimensionally. The osteocytes of the new bone tissue surrounding the DDM formed a network connected by their cellular processes and formed bone tissue. It is also interesting that the cellular processes of the osteocytes extended into the dentinal tubules, and that bone tissue with canaliculi had formed and filled the DDM surface.
format	article
author	Ryuichiro Tanoue Keisuke Ohta Yoshihiro Miyazono Joe Iwanaga Akihiro Koba Toru Natori Osamu Iwamoto Kei-ichiro Nakamura Jingo Kusukawa
author_facet	Ryuichiro Tanoue Keisuke Ohta Yoshihiro Miyazono Joe Iwanaga Akihiro Koba Toru Natori Osamu Iwamoto Kei-ichiro Nakamura Jingo Kusukawa
author_sort	Ryuichiro Tanoue
title	Three-dimensional ultrastructural analysis of the interface between an implanted demineralised dentin matrix and the surrounding newly formed bone
title_short	Three-dimensional ultrastructural analysis of the interface between an implanted demineralised dentin matrix and the surrounding newly formed bone
title_full	Three-dimensional ultrastructural analysis of the interface between an implanted demineralised dentin matrix and the surrounding newly formed bone
title_fullStr	Three-dimensional ultrastructural analysis of the interface between an implanted demineralised dentin matrix and the surrounding newly formed bone
title_full_unstemmed	Three-dimensional ultrastructural analysis of the interface between an implanted demineralised dentin matrix and the surrounding newly formed bone
title_sort	three-dimensional ultrastructural analysis of the interface between an implanted demineralised dentin matrix and the surrounding newly formed bone
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/40f6e0f760a54fadbb98a9f768296e02
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Three-dimensional ultrastructural analysis of the interface between an implanted demineralised dentin matrix and the surrounding newly formed bone

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