Biomimetic Molecular Signaling using DNA Walkers on Microparticles

Abstract We report the release of catalytic DNA walkers from hydrogel microparticles and the detection of those walkers by substrate-coated microparticles. This might be considered a synthetic biology analog of molecular signal release and reception. One type of particles was coated with components...

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Autores principales:	Tulsi Ram Damase, Adam Spencer, Bamidele Samuel, Peter B. Allen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4106554123b74dff9d33637912738b28
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spelling	oai:doaj.org-article:4106554123b74dff9d33637912738b282021-12-02T11:52:34ZBiomimetic Molecular Signaling using DNA Walkers on Microparticles10.1038/s41598-017-04316-12045-2322https://doaj.org/article/4106554123b74dff9d33637912738b282017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04316-1https://doaj.org/toc/2045-2322Abstract We report the release of catalytic DNA walkers from hydrogel microparticles and the detection of those walkers by substrate-coated microparticles. This might be considered a synthetic biology analog of molecular signal release and reception. One type of particles was coated with components of a DNA one-step strand displacement (OSD) reaction to release the walker. A second type of particle was coated with substrate (or “track”) for the molecular walker. We distinguish these particle types using fluorescence barcoding: we synthesized and distinguished multiple particle types with multicolor fluorescence microscopy and automated image analysis software. This represents a step toward amplified, multiplex, and microscopically localized detection based on DNA nanotechnology.Tulsi Ram DamaseAdam SpencerBamidele SamuelPeter B. AllenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tulsi Ram Damase Adam Spencer Bamidele Samuel Peter B. Allen Biomimetic Molecular Signaling using DNA Walkers on Microparticles
description	Abstract We report the release of catalytic DNA walkers from hydrogel microparticles and the detection of those walkers by substrate-coated microparticles. This might be considered a synthetic biology analog of molecular signal release and reception. One type of particles was coated with components of a DNA one-step strand displacement (OSD) reaction to release the walker. A second type of particle was coated with substrate (or “track”) for the molecular walker. We distinguish these particle types using fluorescence barcoding: we synthesized and distinguished multiple particle types with multicolor fluorescence microscopy and automated image analysis software. This represents a step toward amplified, multiplex, and microscopically localized detection based on DNA nanotechnology.
format	article
author	Tulsi Ram Damase Adam Spencer Bamidele Samuel Peter B. Allen
author_facet	Tulsi Ram Damase Adam Spencer Bamidele Samuel Peter B. Allen
author_sort	Tulsi Ram Damase
title	Biomimetic Molecular Signaling using DNA Walkers on Microparticles
title_short	Biomimetic Molecular Signaling using DNA Walkers on Microparticles
title_full	Biomimetic Molecular Signaling using DNA Walkers on Microparticles
title_fullStr	Biomimetic Molecular Signaling using DNA Walkers on Microparticles
title_full_unstemmed	Biomimetic Molecular Signaling using DNA Walkers on Microparticles
title_sort	biomimetic molecular signaling using dna walkers on microparticles
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4106554123b74dff9d33637912738b28
work_keys_str_mv	AT tulsiramdamase biomimeticmolecularsignalingusingdnawalkersonmicroparticles AT adamspencer biomimeticmolecularsignalingusingdnawalkersonmicroparticles AT bamidelesamuel biomimeticmolecularsignalingusingdnawalkersonmicroparticles AT peterballen biomimeticmolecularsignalingusingdnawalkersonmicroparticles
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Biomimetic Molecular Signaling using DNA Walkers on Microparticles

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