Genetic segregation analysis of unsaturated fatty acids content in the filial generations of high-linolenic-acid rapeseed (Brassica napus)

Alpha linolenic acid (ALA) is an essential polyunsaturated fatty acids that can improve human health. Rapeseed is the second largest oil crop in the world but the ALA content in its seed fatty acids is only about 10%. Two rapeseed germplasms YH25005 and R8Q10 with high content (up to 21%) ALA were d...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xiaohan Zhang, Jinglong Lian, Chunyan Dai, Xiaoli Wang, Mingzheng Zhang, Xing Su, Yaohua Cheng, Chengyu Yu
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2021
Materias:	Rapeseed High linolenic acid Fatty acid Genetic analysis Major gene Polygene Genetics QH426-470
Acceso en línea:	https://doaj.org/article/410852cd9cce421cbbebf9efe1350958
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:410852cd9cce421cbbebf9efe1350958
record_format	dspace
spelling	oai:doaj.org-article:410852cd9cce421cbbebf9efe13509582021-11-24T04:28:32ZGenetic segregation analysis of unsaturated fatty acids content in the filial generations of high-linolenic-acid rapeseed (Brassica napus)2096-242810.1016/j.ocsci.2021.10.001https://doaj.org/article/410852cd9cce421cbbebf9efe13509582021-10-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2096242821000518https://doaj.org/toc/2096-2428Alpha linolenic acid (ALA) is an essential polyunsaturated fatty acids that can improve human health. Rapeseed is the second largest oil crop in the world but the ALA content in its seed fatty acids is only about 10%. Two rapeseed germplasms YH25005 and R8Q10 with high content (up to 21%) ALA were developed by intervarietal crossing. They were used as the maternal parent (P1) when crossed with a low ALA parent SW (P2) to produce the seeds of the F1 hybrid, F2, and backcrosses to P1 (BCP1) and P2 (BCP2). A multigeneration joint segregation analysis was conducted to determine major gene + polygene effects of the content of three major unsaturated fatty acids including oleic (OA), linoleic (LA) and ALA. The results showed that, although some genes favorable to ALA accumulation were not allelic in R8Q10 and YH25005, all the inheritances of OA, LA and ALA in YH25005 × SW and R8Q10 × SW followed a genetic model of ‘two pairs of additive major genes + additive-dominant polygenes’. It is suggested that the contents of OA, LA and ALA are closely related and the major genes in the parent SW containing loss-of-function mutations in FAD2 and FAD3 loci had strong effect to reduce ALA and elevate OA. However, total genetic effect of the polygenes was greater than that of the two major genes, especial on ALA content. It indicated that it is necessary to employ a larger F2 population to find the plant that accumulate enough minor-effect polygenes for high ALA content. The results are useful for high ALA rapeseed breeding and future work of gene mapping.Xiaohan ZhangJinglong LianChunyan DaiXiaoli WangMingzheng ZhangXing SuYaohua ChengChengyu YuKeAi Communications Co., Ltd.articleRapeseedHigh linolenic acidFatty acidGenetic analysisMajor genePolygeneGeneticsQH426-470ENOil Crop Science, Vol 6, Iss 4, Pp 169-174 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Rapeseed High linolenic acid Fatty acid Genetic analysis Major gene Polygene Genetics QH426-470
spellingShingle	Rapeseed High linolenic acid Fatty acid Genetic analysis Major gene Polygene Genetics QH426-470 Xiaohan Zhang Jinglong Lian Chunyan Dai Xiaoli Wang Mingzheng Zhang Xing Su Yaohua Cheng Chengyu Yu Genetic segregation analysis of unsaturated fatty acids content in the filial generations of high-linolenic-acid rapeseed (Brassica napus)
description	Alpha linolenic acid (ALA) is an essential polyunsaturated fatty acids that can improve human health. Rapeseed is the second largest oil crop in the world but the ALA content in its seed fatty acids is only about 10%. Two rapeseed germplasms YH25005 and R8Q10 with high content (up to 21%) ALA were developed by intervarietal crossing. They were used as the maternal parent (P1) when crossed with a low ALA parent SW (P2) to produce the seeds of the F1 hybrid, F2, and backcrosses to P1 (BCP1) and P2 (BCP2). A multigeneration joint segregation analysis was conducted to determine major gene + polygene effects of the content of three major unsaturated fatty acids including oleic (OA), linoleic (LA) and ALA. The results showed that, although some genes favorable to ALA accumulation were not allelic in R8Q10 and YH25005, all the inheritances of OA, LA and ALA in YH25005 × SW and R8Q10 × SW followed a genetic model of ‘two pairs of additive major genes + additive-dominant polygenes’. It is suggested that the contents of OA, LA and ALA are closely related and the major genes in the parent SW containing loss-of-function mutations in FAD2 and FAD3 loci had strong effect to reduce ALA and elevate OA. However, total genetic effect of the polygenes was greater than that of the two major genes, especial on ALA content. It indicated that it is necessary to employ a larger F2 population to find the plant that accumulate enough minor-effect polygenes for high ALA content. The results are useful for high ALA rapeseed breeding and future work of gene mapping.
format	article
author	Xiaohan Zhang Jinglong Lian Chunyan Dai Xiaoli Wang Mingzheng Zhang Xing Su Yaohua Cheng Chengyu Yu
author_facet	Xiaohan Zhang Jinglong Lian Chunyan Dai Xiaoli Wang Mingzheng Zhang Xing Su Yaohua Cheng Chengyu Yu
author_sort	Xiaohan Zhang
title	Genetic segregation analysis of unsaturated fatty acids content in the filial generations of high-linolenic-acid rapeseed (Brassica napus)
title_short	Genetic segregation analysis of unsaturated fatty acids content in the filial generations of high-linolenic-acid rapeseed (Brassica napus)
title_full	Genetic segregation analysis of unsaturated fatty acids content in the filial generations of high-linolenic-acid rapeseed (Brassica napus)
title_fullStr	Genetic segregation analysis of unsaturated fatty acids content in the filial generations of high-linolenic-acid rapeseed (Brassica napus)
title_full_unstemmed	Genetic segregation analysis of unsaturated fatty acids content in the filial generations of high-linolenic-acid rapeseed (Brassica napus)
title_sort	genetic segregation analysis of unsaturated fatty acids content in the filial generations of high-linolenic-acid rapeseed (brassica napus)
publisher	KeAi Communications Co., Ltd.
publishDate	2021
url	https://doaj.org/article/410852cd9cce421cbbebf9efe1350958
work_keys_str_mv	AT xiaohanzhang geneticsegregationanalysisofunsaturatedfattyacidscontentinthefilialgenerationsofhighlinolenicacidrapeseedbrassicanapus AT jinglonglian geneticsegregationanalysisofunsaturatedfattyacidscontentinthefilialgenerationsofhighlinolenicacidrapeseedbrassicanapus AT chunyandai geneticsegregationanalysisofunsaturatedfattyacidscontentinthefilialgenerationsofhighlinolenicacidrapeseedbrassicanapus AT xiaoliwang geneticsegregationanalysisofunsaturatedfattyacidscontentinthefilialgenerationsofhighlinolenicacidrapeseedbrassicanapus AT mingzhengzhang geneticsegregationanalysisofunsaturatedfattyacidscontentinthefilialgenerationsofhighlinolenicacidrapeseedbrassicanapus AT xingsu geneticsegregationanalysisofunsaturatedfattyacidscontentinthefilialgenerationsofhighlinolenicacidrapeseedbrassicanapus AT yaohuacheng geneticsegregationanalysisofunsaturatedfattyacidscontentinthefilialgenerationsofhighlinolenicacidrapeseedbrassicanapus AT chengyuyu geneticsegregationanalysisofunsaturatedfattyacidscontentinthefilialgenerationsofhighlinolenicacidrapeseedbrassicanapus
_version_	1718415971362799616

Genetic segregation analysis of unsaturated fatty acids content in the filial generations of high-linolenic-acid rapeseed (Brassica napus)

Ejemplares similares