Cinnamaldehyde and Doxorubicin Co-Loaded Graphene Oxide Wrapped Mesoporous Silica Nanoparticles for Enhanced MCF-7 Cell Apoptosis

Kai Dong,* Zhuang-Zhuang Zhao,* Jian Kang, Lei-Ruo Lin, Wen-Ting Chen, Jin-Xi Liu, Xiang-Long Wu, Ting-Li Lu Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an, Shaanxi, People’s Republic of China*These aut...

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Autores principales:	Dong K, Zhao ZZ, Kang J, Lin LR, Chen WT, Liu JX, Wu XL, Lu TL
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	mesoporous silica nanoparticle graphene oxide hyaluronic acid cinnamaldehyde doxorubicin Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/410ee62956a542d89ac0b83840fdaa31
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spelling	oai:doaj.org-article:410ee62956a542d89ac0b83840fdaa312021-12-02T10:34:34ZCinnamaldehyde and Doxorubicin Co-Loaded Graphene Oxide Wrapped Mesoporous Silica Nanoparticles for Enhanced MCF-7 Cell Apoptosis1178-2013https://doaj.org/article/410ee62956a542d89ac0b83840fdaa312020-12-01T00:00:00Zhttps://www.dovepress.com/cinnamaldehyde-and-doxorubicin-co-loaded-graphene-oxide-wrapped-mesopo-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Kai Dong,* Zhuang-Zhuang Zhao,* Jian Kang, Lei-Ruo Lin, Wen-Ting Chen, Jin-Xi Liu, Xiang-Long Wu, Ting-Li Lu Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an, Shaanxi, People’s Republic of China*These authors contributed equally to this workCorrespondence: Ting-Li LuKey Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an, Shaanxi, People’s Republic of ChinaTel/Fax +86-29-88460332Email lutinglixinxin@nwpu.edu.cnBackground: Combined chemotherapy is often affected by the different physicochemical properties of chemotherapeutic drugs, which should be improved by the reasonable design of co-loaded preparations.Purpose: A kind of simple but practical graphene oxide (GO) wrapped mesoporous silica nanoparticles (MSN) modified with hyaluronic acid (MSN@GO-HA) were developed for the co-delivery of cinnamaldehyde (CA) and doxorubicin (DOX), in order to enhance their combined treatment on tumor cells and reduce their application defects.Methods: The MSNCA@GODOX-HA was constructed by MSNCA (loading CA via physical diffusion) and GODOX-HA (modified with HA and loading DOX via π–π stacking) through the electrostatic adsorption, followed by the physicochemical characterization, serum stability and in vitro release study. Cytotoxicity on different cells was detected, followed by the tumor cell uptake tests. The intracellular reactive oxygen species (ROS) changes, mitochondrial functions and activities of caspase-3/-9 in MCF-7 cells were also evaluated, respectively.Results: The MSNCA@GODOX-HA nanoparticles kept stable in FBS solution and achieved pH-responsive release behavior, which was beneficial to increase the accumulation of CA and DOX in tumor cells to enhance the treatment. MSNCA@GODOX-HA exerted higher cytotoxicity to MCF-7 human breast cancer cells than H9c2 cardiac myocyte cells, which were not only attributed to the active targeting to tumor cells by HA, but also related with the activation of intrinsic apoptotic pathway in MCF-7 cells induced by CA, which was mediated by the specific ROS signal amplification and the interference with mitochondrial function. Moreover, the efficacy of DOX was also enhanced by the above process.Conclusion: The establishment of the MSNCA@GODOX-HA nanoparticles played a role in promoting strengths and restricting shortcomings of CA and DOX, thereby exerting their function and achieving efficient treatment against cancer.Keywords: mesoporous silica nanoparticle, graphene oxide, hyaluronic acid, cinnamaldehyde, doxorubicinDong KZhao ZZKang JLin LRChen WTLiu JXWu XLLu TLDove Medical Pressarticlemesoporous silica nanoparticlegraphene oxidehyaluronic acidcinnamaldehydedoxorubicinMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 10285-10304 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	mesoporous silica nanoparticle graphene oxide hyaluronic acid cinnamaldehyde doxorubicin Medicine (General) R5-920
spellingShingle	mesoporous silica nanoparticle graphene oxide hyaluronic acid cinnamaldehyde doxorubicin Medicine (General) R5-920 Dong K Zhao ZZ Kang J Lin LR Chen WT Liu JX Wu XL Lu TL Cinnamaldehyde and Doxorubicin Co-Loaded Graphene Oxide Wrapped Mesoporous Silica Nanoparticles for Enhanced MCF-7 Cell Apoptosis
description	Kai Dong,* Zhuang-Zhuang Zhao,* Jian Kang, Lei-Ruo Lin, Wen-Ting Chen, Jin-Xi Liu, Xiang-Long Wu, Ting-Li Lu Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an, Shaanxi, People’s Republic of China*These authors contributed equally to this workCorrespondence: Ting-Li LuKey Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an, Shaanxi, People’s Republic of ChinaTel/Fax +86-29-88460332Email lutinglixinxin@nwpu.edu.cnBackground: Combined chemotherapy is often affected by the different physicochemical properties of chemotherapeutic drugs, which should be improved by the reasonable design of co-loaded preparations.Purpose: A kind of simple but practical graphene oxide (GO) wrapped mesoporous silica nanoparticles (MSN) modified with hyaluronic acid (MSN@GO-HA) were developed for the co-delivery of cinnamaldehyde (CA) and doxorubicin (DOX), in order to enhance their combined treatment on tumor cells and reduce their application defects.Methods: The MSNCA@GODOX-HA was constructed by MSNCA (loading CA via physical diffusion) and GODOX-HA (modified with HA and loading DOX via π–π stacking) through the electrostatic adsorption, followed by the physicochemical characterization, serum stability and in vitro release study. Cytotoxicity on different cells was detected, followed by the tumor cell uptake tests. The intracellular reactive oxygen species (ROS) changes, mitochondrial functions and activities of caspase-3/-9 in MCF-7 cells were also evaluated, respectively.Results: The MSNCA@GODOX-HA nanoparticles kept stable in FBS solution and achieved pH-responsive release behavior, which was beneficial to increase the accumulation of CA and DOX in tumor cells to enhance the treatment. MSNCA@GODOX-HA exerted higher cytotoxicity to MCF-7 human breast cancer cells than H9c2 cardiac myocyte cells, which were not only attributed to the active targeting to tumor cells by HA, but also related with the activation of intrinsic apoptotic pathway in MCF-7 cells induced by CA, which was mediated by the specific ROS signal amplification and the interference with mitochondrial function. Moreover, the efficacy of DOX was also enhanced by the above process.Conclusion: The establishment of the MSNCA@GODOX-HA nanoparticles played a role in promoting strengths and restricting shortcomings of CA and DOX, thereby exerting their function and achieving efficient treatment against cancer.Keywords: mesoporous silica nanoparticle, graphene oxide, hyaluronic acid, cinnamaldehyde, doxorubicin
format	article
author	Dong K Zhao ZZ Kang J Lin LR Chen WT Liu JX Wu XL Lu TL
author_facet	Dong K Zhao ZZ Kang J Lin LR Chen WT Liu JX Wu XL Lu TL
author_sort	Dong K
title	Cinnamaldehyde and Doxorubicin Co-Loaded Graphene Oxide Wrapped Mesoporous Silica Nanoparticles for Enhanced MCF-7 Cell Apoptosis
title_short	Cinnamaldehyde and Doxorubicin Co-Loaded Graphene Oxide Wrapped Mesoporous Silica Nanoparticles for Enhanced MCF-7 Cell Apoptosis
title_full	Cinnamaldehyde and Doxorubicin Co-Loaded Graphene Oxide Wrapped Mesoporous Silica Nanoparticles for Enhanced MCF-7 Cell Apoptosis
title_fullStr	Cinnamaldehyde and Doxorubicin Co-Loaded Graphene Oxide Wrapped Mesoporous Silica Nanoparticles for Enhanced MCF-7 Cell Apoptosis
title_full_unstemmed	Cinnamaldehyde and Doxorubicin Co-Loaded Graphene Oxide Wrapped Mesoporous Silica Nanoparticles for Enhanced MCF-7 Cell Apoptosis
title_sort	cinnamaldehyde and doxorubicin co-loaded graphene oxide wrapped mesoporous silica nanoparticles for enhanced mcf-7 cell apoptosis
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/410ee62956a542d89ac0b83840fdaa31
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Cinnamaldehyde and Doxorubicin Co-Loaded Graphene Oxide Wrapped Mesoporous Silica Nanoparticles for Enhanced MCF-7 Cell Apoptosis

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