Assessing Multiplex Tiling PCR Sequencing Approaches for Detecting Genomic Variants of SARS-CoV-2 in Municipal Wastewater

Monitoring the genomic characteristics of the SARS-CoV-2 virus circulating in a population can shed important insights into epidemiological aspects of the COVID-19 outbreak. Sequencing every clinical patient sample in a highly populous area is a difficult feat, and thus sequencing SARS-CoV-2 RNA in...

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Autores principales:	Xuan Lin, Melissa Glier, Kevin Kuchinski, Tenysha Ross-Van Mierlo, David McVea, John R. Tyson, Natalie Prystajecky, Ryan M. Ziels
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/410f3697c44a482bb9f5745150ec858b
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spelling	oai:doaj.org-article:410f3697c44a482bb9f5745150ec858b2021-12-02T19:23:31ZAssessing Multiplex Tiling PCR Sequencing Approaches for Detecting Genomic Variants of SARS-CoV-2 in Municipal Wastewater2379-507710.1128/mSystems.01068-21https://doaj.org/article/410f3697c44a482bb9f5745150ec858b2021-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.01068-21https://doaj.org/toc/2379-5077 Monitoring the genomic characteristics of the SARS-CoV-2 virus circulating in a population can shed important insights into epidemiological aspects of the COVID-19 outbreak. Sequencing every clinical patient sample in a highly populous area is a difficult feat, and thus sequencing SARS-CoV-2 RNA in municipal wastewater offers great promise to augment genomic surveillance by characterizing a pooled population sample matrix, particularly during an escalating outbreak.Xuan LinMelissa GlierKevin KuchinskiTenysha Ross-Van MierloDavid McVeaJohn R. TysonNatalie PrystajeckyRyan M. ZielsAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmSystems, Vol 6, Iss 5 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Xuan Lin Melissa Glier Kevin Kuchinski Tenysha Ross-Van Mierlo David McVea John R. Tyson Natalie Prystajecky Ryan M. Ziels Assessing Multiplex Tiling PCR Sequencing Approaches for Detecting Genomic Variants of SARS-CoV-2 in Municipal Wastewater
description	Monitoring the genomic characteristics of the SARS-CoV-2 virus circulating in a population can shed important insights into epidemiological aspects of the COVID-19 outbreak. Sequencing every clinical patient sample in a highly populous area is a difficult feat, and thus sequencing SARS-CoV-2 RNA in municipal wastewater offers great promise to augment genomic surveillance by characterizing a pooled population sample matrix, particularly during an escalating outbreak.
format	article
author	Xuan Lin Melissa Glier Kevin Kuchinski Tenysha Ross-Van Mierlo David McVea John R. Tyson Natalie Prystajecky Ryan M. Ziels
author_facet	Xuan Lin Melissa Glier Kevin Kuchinski Tenysha Ross-Van Mierlo David McVea John R. Tyson Natalie Prystajecky Ryan M. Ziels
author_sort	Xuan Lin
title	Assessing Multiplex Tiling PCR Sequencing Approaches for Detecting Genomic Variants of SARS-CoV-2 in Municipal Wastewater
title_short	Assessing Multiplex Tiling PCR Sequencing Approaches for Detecting Genomic Variants of SARS-CoV-2 in Municipal Wastewater
title_full	Assessing Multiplex Tiling PCR Sequencing Approaches for Detecting Genomic Variants of SARS-CoV-2 in Municipal Wastewater
title_fullStr	Assessing Multiplex Tiling PCR Sequencing Approaches for Detecting Genomic Variants of SARS-CoV-2 in Municipal Wastewater
title_full_unstemmed	Assessing Multiplex Tiling PCR Sequencing Approaches for Detecting Genomic Variants of SARS-CoV-2 in Municipal Wastewater
title_sort	assessing multiplex tiling pcr sequencing approaches for detecting genomic variants of sars-cov-2 in municipal wastewater
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/410f3697c44a482bb9f5745150ec858b
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Assessing Multiplex Tiling PCR Sequencing Approaches for Detecting Genomic Variants of SARS-CoV-2 in Municipal Wastewater

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