Quantitative imaging of single upconversion nanoparticles in biological tissue.

The unique luminescent properties of new-generation synthetic nanomaterials, upconversion nanoparticles (UCNPs), enabled high-contrast optical biomedical imaging by suppressing the crowded background of biological tissue autofluorescence and evading high tissue absorption. This raised high expectati...

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Autores principales:	Annemarie Nadort, Varun K A Sreenivasan, Zhen Song, Ekaterina A Grebenik, Andrei V Nechaev, Vladimir A Semchishen, Vladislav Y Panchenko, Andrei V Zvyagin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4115a5de3ebe44e8af59dbd198e0de5b
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spelling	oai:doaj.org-article:4115a5de3ebe44e8af59dbd198e0de5b2021-11-18T07:45:52ZQuantitative imaging of single upconversion nanoparticles in biological tissue.1932-620310.1371/journal.pone.0063292https://doaj.org/article/4115a5de3ebe44e8af59dbd198e0de5b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23691012/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The unique luminescent properties of new-generation synthetic nanomaterials, upconversion nanoparticles (UCNPs), enabled high-contrast optical biomedical imaging by suppressing the crowded background of biological tissue autofluorescence and evading high tissue absorption. This raised high expectations on the UCNP utilities for intracellular and deep tissue imaging, such as whole animal imaging. At the same time, the critical nonlinear dependence of the UCNP luminescence on the excitation intensity results in dramatic signal reduction at (∼1 cm) depth in biological tissue. Here, we report on the experimental and theoretical investigation of this trade-off aiming at the identification of optimal application niches of UCNPs e.g. biological liquids and subsurface tissue layers. As an example of such applications, we report on single UCNP imaging through a layer of hemolyzed blood. To extend this result towards in vivo applications, we quantified the optical properties of single UCNPs and theoretically analyzed the prospects of single-particle detectability in live scattering and absorbing bio-tissue using a human skin model. The model predicts that a single 70-nm UCNP would be detectable at skin depths up to 400 µm, unlike a hardly detectable single fluorescent (fluorescein) dye molecule. UCNP-assisted imaging in the ballistic regime thus allows for excellent applications niches, where high sensitivity is the key requirement.Annemarie NadortVarun K A SreenivasanZhen SongEkaterina A GrebenikAndrei V NechaevVladimir A SemchishenVladislav Y PanchenkoAndrei V ZvyaginPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e63292 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Annemarie Nadort Varun K A Sreenivasan Zhen Song Ekaterina A Grebenik Andrei V Nechaev Vladimir A Semchishen Vladislav Y Panchenko Andrei V Zvyagin Quantitative imaging of single upconversion nanoparticles in biological tissue.
description	The unique luminescent properties of new-generation synthetic nanomaterials, upconversion nanoparticles (UCNPs), enabled high-contrast optical biomedical imaging by suppressing the crowded background of biological tissue autofluorescence and evading high tissue absorption. This raised high expectations on the UCNP utilities for intracellular and deep tissue imaging, such as whole animal imaging. At the same time, the critical nonlinear dependence of the UCNP luminescence on the excitation intensity results in dramatic signal reduction at (∼1 cm) depth in biological tissue. Here, we report on the experimental and theoretical investigation of this trade-off aiming at the identification of optimal application niches of UCNPs e.g. biological liquids and subsurface tissue layers. As an example of such applications, we report on single UCNP imaging through a layer of hemolyzed blood. To extend this result towards in vivo applications, we quantified the optical properties of single UCNPs and theoretically analyzed the prospects of single-particle detectability in live scattering and absorbing bio-tissue using a human skin model. The model predicts that a single 70-nm UCNP would be detectable at skin depths up to 400 µm, unlike a hardly detectable single fluorescent (fluorescein) dye molecule. UCNP-assisted imaging in the ballistic regime thus allows for excellent applications niches, where high sensitivity is the key requirement.
format	article
author	Annemarie Nadort Varun K A Sreenivasan Zhen Song Ekaterina A Grebenik Andrei V Nechaev Vladimir A Semchishen Vladislav Y Panchenko Andrei V Zvyagin
author_facet	Annemarie Nadort Varun K A Sreenivasan Zhen Song Ekaterina A Grebenik Andrei V Nechaev Vladimir A Semchishen Vladislav Y Panchenko Andrei V Zvyagin
author_sort	Annemarie Nadort
title	Quantitative imaging of single upconversion nanoparticles in biological tissue.
title_short	Quantitative imaging of single upconversion nanoparticles in biological tissue.
title_full	Quantitative imaging of single upconversion nanoparticles in biological tissue.
title_fullStr	Quantitative imaging of single upconversion nanoparticles in biological tissue.
title_full_unstemmed	Quantitative imaging of single upconversion nanoparticles in biological tissue.
title_sort	quantitative imaging of single upconversion nanoparticles in biological tissue.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/4115a5de3ebe44e8af59dbd198e0de5b
work_keys_str_mv	AT annemarienadort quantitativeimagingofsingleupconversionnanoparticlesinbiologicaltissue AT varunkasreenivasan quantitativeimagingofsingleupconversionnanoparticlesinbiologicaltissue AT zhensong quantitativeimagingofsingleupconversionnanoparticlesinbiologicaltissue AT ekaterinaagrebenik quantitativeimagingofsingleupconversionnanoparticlesinbiologicaltissue AT andreivnechaev quantitativeimagingofsingleupconversionnanoparticlesinbiologicaltissue AT vladimirasemchishen quantitativeimagingofsingleupconversionnanoparticlesinbiologicaltissue AT vladislavypanchenko quantitativeimagingofsingleupconversionnanoparticlesinbiologicaltissue AT andreivzvyagin quantitativeimagingofsingleupconversionnanoparticlesinbiologicaltissue
_version_	1718422965026029568

Quantitative imaging of single upconversion nanoparticles in biological tissue.

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