Differentiation of embryonic stem cells into a putative hair cell-progenitor cells via co-culture with HEI-OC1 cells

Abstract Several studies have shown how different cell lines can influence the differentiation of stem cells through co-culture systems. The House Ear Institute-Organ of Corti 1 (HEI-OC1) is considered an important cell line for in vitro auditory research. However, it is unknown if HEI-OC1 cells can...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nathaniel T. Carpena, So-Young Chang, Celine D. G. Abueva, Jae Yun Jung, Min Young Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/411a318c030d4e1692f5b48b7aa63892
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:411a318c030d4e1692f5b48b7aa63892
record_format	dspace
spelling	oai:doaj.org-article:411a318c030d4e1692f5b48b7aa638922021-12-02T16:14:46ZDifferentiation of embryonic stem cells into a putative hair cell-progenitor cells via co-culture with HEI-OC1 cells10.1038/s41598-021-93049-32045-2322https://doaj.org/article/411a318c030d4e1692f5b48b7aa638922021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93049-3https://doaj.org/toc/2045-2322Abstract Several studies have shown how different cell lines can influence the differentiation of stem cells through co-culture systems. The House Ear Institute-Organ of Corti 1 (HEI-OC1) is considered an important cell line for in vitro auditory research. However, it is unknown if HEI-OC1 cells can promote the differentiation of embryonic stem cells (ESCs). In this study, we investigated whether co-culture of ESCs with HEI-OC1 cells promotes differentiation. To this end, we developed a co-culture system of mouse ESCs with HEI-OC1 cells. Dissociated or embryonic bodies (EBs) of ESCs were introduced to a conditioned and inactivated confluent layer of HEI-OC1 cells for 14 days. The dissociated ESCs coalesced into an EB-like form that was smaller than the co-cultured EBs. Contact co-culture generated cells expressing several otic progenitor markers as well as hair cell specific markers. ESCs and EBs were also cultured in non-contact setup but using conditioned medium from HEI-OC1 cells, indicating that soluble factors alone could have a similar effect. The ESCs did not form into aggregates but were still Myo7a-positive, while the EBs degenerated. However, in the fully differentiated EBs, evidence to prove mature differentiation of inner ear hair cell was still rudimentary. Nevertheless, these results suggest that cellular interactions between ESCs and HEI-OC1 cells may both stimulate ESC differentiation.Nathaniel T. CarpenaSo-Young ChangCeline D. G. AbuevaJae Yun JungMin Young LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nathaniel T. Carpena So-Young Chang Celine D. G. Abueva Jae Yun Jung Min Young Lee Differentiation of embryonic stem cells into a putative hair cell-progenitor cells via co-culture with HEI-OC1 cells
description	Abstract Several studies have shown how different cell lines can influence the differentiation of stem cells through co-culture systems. The House Ear Institute-Organ of Corti 1 (HEI-OC1) is considered an important cell line for in vitro auditory research. However, it is unknown if HEI-OC1 cells can promote the differentiation of embryonic stem cells (ESCs). In this study, we investigated whether co-culture of ESCs with HEI-OC1 cells promotes differentiation. To this end, we developed a co-culture system of mouse ESCs with HEI-OC1 cells. Dissociated or embryonic bodies (EBs) of ESCs were introduced to a conditioned and inactivated confluent layer of HEI-OC1 cells for 14 days. The dissociated ESCs coalesced into an EB-like form that was smaller than the co-cultured EBs. Contact co-culture generated cells expressing several otic progenitor markers as well as hair cell specific markers. ESCs and EBs were also cultured in non-contact setup but using conditioned medium from HEI-OC1 cells, indicating that soluble factors alone could have a similar effect. The ESCs did not form into aggregates but were still Myo7a-positive, while the EBs degenerated. However, in the fully differentiated EBs, evidence to prove mature differentiation of inner ear hair cell was still rudimentary. Nevertheless, these results suggest that cellular interactions between ESCs and HEI-OC1 cells may both stimulate ESC differentiation.
format	article
author	Nathaniel T. Carpena So-Young Chang Celine D. G. Abueva Jae Yun Jung Min Young Lee
author_facet	Nathaniel T. Carpena So-Young Chang Celine D. G. Abueva Jae Yun Jung Min Young Lee
author_sort	Nathaniel T. Carpena
title	Differentiation of embryonic stem cells into a putative hair cell-progenitor cells via co-culture with HEI-OC1 cells
title_short	Differentiation of embryonic stem cells into a putative hair cell-progenitor cells via co-culture with HEI-OC1 cells
title_full	Differentiation of embryonic stem cells into a putative hair cell-progenitor cells via co-culture with HEI-OC1 cells
title_fullStr	Differentiation of embryonic stem cells into a putative hair cell-progenitor cells via co-culture with HEI-OC1 cells
title_full_unstemmed	Differentiation of embryonic stem cells into a putative hair cell-progenitor cells via co-culture with HEI-OC1 cells
title_sort	differentiation of embryonic stem cells into a putative hair cell-progenitor cells via co-culture with hei-oc1 cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/411a318c030d4e1692f5b48b7aa63892
work_keys_str_mv	AT nathanieltcarpena differentiationofembryonicstemcellsintoaputativehaircellprogenitorcellsviacoculturewithheioc1cells AT soyoungchang differentiationofembryonicstemcellsintoaputativehaircellprogenitorcellsviacoculturewithheioc1cells AT celinedgabueva differentiationofembryonicstemcellsintoaputativehaircellprogenitorcellsviacoculturewithheioc1cells AT jaeyunjung differentiationofembryonicstemcellsintoaputativehaircellprogenitorcellsviacoculturewithheioc1cells AT minyounglee differentiationofembryonicstemcellsintoaputativehaircellprogenitorcellsviacoculturewithheioc1cells
_version_	1718384286637228032

Differentiation of embryonic stem cells into a putative hair cell-progenitor cells via co-culture with HEI-OC1 cells

Ejemplares similares