Shedding of host autophagic proteins from the parasitophorous vacuolar membrane of Plasmodium berghei

Abstract The hepatic stage of the malaria parasite Plasmodium is accompanied by an autophagy-mediated host response directly targeting the parasitophorous vacuolar membrane (PVM) harbouring the parasite. Removal of the PVM-associated autophagic proteins such as ubiquitin, p62, and LC3 correlates wit...

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Autores principales:	Carolina Agop-Nersesian, Mariana De Niz, Livia Niklaus, Monica Prado, Nina Eickel, Volker T. Heussler
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/411edef25ce24e65a8d1f15f209af4b3
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spelling	oai:doaj.org-article:411edef25ce24e65a8d1f15f209af4b32021-12-02T12:32:54ZShedding of host autophagic proteins from the parasitophorous vacuolar membrane of Plasmodium berghei10.1038/s41598-017-02156-72045-2322https://doaj.org/article/411edef25ce24e65a8d1f15f209af4b32017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02156-7https://doaj.org/toc/2045-2322Abstract The hepatic stage of the malaria parasite Plasmodium is accompanied by an autophagy-mediated host response directly targeting the parasitophorous vacuolar membrane (PVM) harbouring the parasite. Removal of the PVM-associated autophagic proteins such as ubiquitin, p62, and LC3 correlates with parasite survival. Yet, it is unclear how Plasmodium avoids the deleterious effects of selective autophagy. Here we show that parasites trap host autophagic factors in the tubovesicular network (TVN), an expansion of the PVM into the host cytoplasm. In proliferating parasites, PVM-associated LC3 becomes immediately redirected into the TVN, where it accumulates distally from the parasite’s replicative centre. Finally, the host factors are shed as vesicles into the host cytoplasm. This strategy may enable the parasite to balance the benefits of the enhanced host catabolic activity with the risk of being eliminated by the cell’s cytosolic immune defence.Carolina Agop-NersesianMariana De NizLivia NiklausMonica PradoNina EickelVolker T. HeusslerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carolina Agop-Nersesian Mariana De Niz Livia Niklaus Monica Prado Nina Eickel Volker T. Heussler Shedding of host autophagic proteins from the parasitophorous vacuolar membrane of Plasmodium berghei
description	Abstract The hepatic stage of the malaria parasite Plasmodium is accompanied by an autophagy-mediated host response directly targeting the parasitophorous vacuolar membrane (PVM) harbouring the parasite. Removal of the PVM-associated autophagic proteins such as ubiquitin, p62, and LC3 correlates with parasite survival. Yet, it is unclear how Plasmodium avoids the deleterious effects of selective autophagy. Here we show that parasites trap host autophagic factors in the tubovesicular network (TVN), an expansion of the PVM into the host cytoplasm. In proliferating parasites, PVM-associated LC3 becomes immediately redirected into the TVN, where it accumulates distally from the parasite’s replicative centre. Finally, the host factors are shed as vesicles into the host cytoplasm. This strategy may enable the parasite to balance the benefits of the enhanced host catabolic activity with the risk of being eliminated by the cell’s cytosolic immune defence.
format	article
author	Carolina Agop-Nersesian Mariana De Niz Livia Niklaus Monica Prado Nina Eickel Volker T. Heussler
author_facet	Carolina Agop-Nersesian Mariana De Niz Livia Niklaus Monica Prado Nina Eickel Volker T. Heussler
author_sort	Carolina Agop-Nersesian
title	Shedding of host autophagic proteins from the parasitophorous vacuolar membrane of Plasmodium berghei
title_short	Shedding of host autophagic proteins from the parasitophorous vacuolar membrane of Plasmodium berghei
title_full	Shedding of host autophagic proteins from the parasitophorous vacuolar membrane of Plasmodium berghei
title_fullStr	Shedding of host autophagic proteins from the parasitophorous vacuolar membrane of Plasmodium berghei
title_full_unstemmed	Shedding of host autophagic proteins from the parasitophorous vacuolar membrane of Plasmodium berghei
title_sort	shedding of host autophagic proteins from the parasitophorous vacuolar membrane of plasmodium berghei
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/411edef25ce24e65a8d1f15f209af4b3
work_keys_str_mv	AT carolinaagopnersesian sheddingofhostautophagicproteinsfromtheparasitophorousvacuolarmembraneofplasmodiumberghei AT marianadeniz sheddingofhostautophagicproteinsfromtheparasitophorousvacuolarmembraneofplasmodiumberghei AT livianiklaus sheddingofhostautophagicproteinsfromtheparasitophorousvacuolarmembraneofplasmodiumberghei AT monicaprado sheddingofhostautophagicproteinsfromtheparasitophorousvacuolarmembraneofplasmodiumberghei AT ninaeickel sheddingofhostautophagicproteinsfromtheparasitophorousvacuolarmembraneofplasmodiumberghei AT volkertheussler sheddingofhostautophagicproteinsfromtheparasitophorousvacuolarmembraneofplasmodiumberghei
_version_	1718393912225169408

Shedding of host autophagic proteins from the parasitophorous vacuolar membrane of Plasmodium berghei

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