Quantitative photo activated localization microscopy: unraveling the effects of photoblinking.

In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way...

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Autores principales:	Paolo Annibale, Stefano Vanni, Marco Scarselli, Ursula Rothlisberger, Aleksandra Radenovic
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/412d73c9a0b74315b24252f3c51de502
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spelling	oai:doaj.org-article:412d73c9a0b74315b24252f3c51de5022021-11-18T06:49:22ZQuantitative photo activated localization microscopy: unraveling the effects of photoblinking.1932-620310.1371/journal.pone.0022678https://doaj.org/article/412d73c9a0b74315b24252f3c51de5022011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21818365/?tool=EBIhttps://doaj.org/toc/1932-6203In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way to quantify proteins at the single-cell level from PALM images. We apply this method to determine the amount of β2 adrenergic receptor, a prototypical G Protein Coupled Receptor, expressed on the plasma membrane of HeLa cells.Paolo AnnibaleStefano VanniMarco ScarselliUrsula RothlisbergerAleksandra RadenovicPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e22678 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Paolo Annibale Stefano Vanni Marco Scarselli Ursula Rothlisberger Aleksandra Radenovic Quantitative photo activated localization microscopy: unraveling the effects of photoblinking.
description	In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way to quantify proteins at the single-cell level from PALM images. We apply this method to determine the amount of β2 adrenergic receptor, a prototypical G Protein Coupled Receptor, expressed on the plasma membrane of HeLa cells.
format	article
author	Paolo Annibale Stefano Vanni Marco Scarselli Ursula Rothlisberger Aleksandra Radenovic
author_facet	Paolo Annibale Stefano Vanni Marco Scarselli Ursula Rothlisberger Aleksandra Radenovic
author_sort	Paolo Annibale
title	Quantitative photo activated localization microscopy: unraveling the effects of photoblinking.
title_short	Quantitative photo activated localization microscopy: unraveling the effects of photoblinking.
title_full	Quantitative photo activated localization microscopy: unraveling the effects of photoblinking.
title_fullStr	Quantitative photo activated localization microscopy: unraveling the effects of photoblinking.
title_full_unstemmed	Quantitative photo activated localization microscopy: unraveling the effects of photoblinking.
title_sort	quantitative photo activated localization microscopy: unraveling the effects of photoblinking.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/412d73c9a0b74315b24252f3c51de502
work_keys_str_mv	AT paoloannibale quantitativephotoactivatedlocalizationmicroscopyunravelingtheeffectsofphotoblinking AT stefanovanni quantitativephotoactivatedlocalizationmicroscopyunravelingtheeffectsofphotoblinking AT marcoscarselli quantitativephotoactivatedlocalizationmicroscopyunravelingtheeffectsofphotoblinking AT ursularothlisberger quantitativephotoactivatedlocalizationmicroscopyunravelingtheeffectsofphotoblinking AT aleksandraradenovic quantitativephotoactivatedlocalizationmicroscopyunravelingtheeffectsofphotoblinking
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Quantitative photo activated localization microscopy: unraveling the effects of photoblinking.

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