Quantitative photo activated localization microscopy: unraveling the effects of photoblinking.
In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way...
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Autores principales: | Paolo Annibale, Stefano Vanni, Marco Scarselli, Ursula Rothlisberger, Aleksandra Radenovic |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Public Library of Science (PLoS)
2011
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Acceso en línea: | https://doaj.org/article/412d73c9a0b74315b24252f3c51de502 |
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