Regulation of GSTu1-mediated insecticide resistance in Plutella xylostella by miRNA and lncRNA.
The evolution of resistance to insecticides is well known to be closely associated with the overexpression of detoxifying enzymes. Although the role of glutathione S-transferase (GST) genes in insecticide resistance has been widely reported, the underlying regulatory mechanisms are poorly understood...
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2021
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oai:doaj.org-article:41352dccc1714721b185d971a9cf67c92021-12-02T20:03:29ZRegulation of GSTu1-mediated insecticide resistance in Plutella xylostella by miRNA and lncRNA.1553-73901553-740410.1371/journal.pgen.1009888https://doaj.org/article/41352dccc1714721b185d971a9cf67c92021-10-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009888https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404The evolution of resistance to insecticides is well known to be closely associated with the overexpression of detoxifying enzymes. Although the role of glutathione S-transferase (GST) genes in insecticide resistance has been widely reported, the underlying regulatory mechanisms are poorly understood. Here, one GST gene (GSTu1) and its antisense transcript (lnc-GSTu1-AS) were identified and cloned, and both of them were upregulated in several chlorantraniliprole-resistant Plutella xylostella populations. GSTu1 was confirmed to be involved in chlorantraniliprole resistance by direct degradation of this insecticide. Furthermore, we demonstrated that lnc-GSTu1-AS interacted with GSTu1 by forming an RNA duplex, which masked the binding site of miR-8525-5p at the GSTu1-3'UTR. In summary, we revealed that lnc-GSTu1-AS maintained the mRNA stability of GSTu1 by preventing its degradation that could have been induced by miR-8525-5p and thus increased the resistance of P. xylostella to chlorantraniliprole. Our findings reveal a new noncoding RNA-mediated pathway that regulates the expression of detoxifying enzymes in insecticide-resistant insects and offer opportunities for the further understanding of the mechanisms of insecticide and drug resistance.Bin ZhuLinhong LiRui WeiPei LiangXiwu GaoPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 10, p e1009888 (2021) |
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Genetics QH426-470 |
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Genetics QH426-470 Bin Zhu Linhong Li Rui Wei Pei Liang Xiwu Gao Regulation of GSTu1-mediated insecticide resistance in Plutella xylostella by miRNA and lncRNA. |
description |
The evolution of resistance to insecticides is well known to be closely associated with the overexpression of detoxifying enzymes. Although the role of glutathione S-transferase (GST) genes in insecticide resistance has been widely reported, the underlying regulatory mechanisms are poorly understood. Here, one GST gene (GSTu1) and its antisense transcript (lnc-GSTu1-AS) were identified and cloned, and both of them were upregulated in several chlorantraniliprole-resistant Plutella xylostella populations. GSTu1 was confirmed to be involved in chlorantraniliprole resistance by direct degradation of this insecticide. Furthermore, we demonstrated that lnc-GSTu1-AS interacted with GSTu1 by forming an RNA duplex, which masked the binding site of miR-8525-5p at the GSTu1-3'UTR. In summary, we revealed that lnc-GSTu1-AS maintained the mRNA stability of GSTu1 by preventing its degradation that could have been induced by miR-8525-5p and thus increased the resistance of P. xylostella to chlorantraniliprole. Our findings reveal a new noncoding RNA-mediated pathway that regulates the expression of detoxifying enzymes in insecticide-resistant insects and offer opportunities for the further understanding of the mechanisms of insecticide and drug resistance. |
format |
article |
author |
Bin Zhu Linhong Li Rui Wei Pei Liang Xiwu Gao |
author_facet |
Bin Zhu Linhong Li Rui Wei Pei Liang Xiwu Gao |
author_sort |
Bin Zhu |
title |
Regulation of GSTu1-mediated insecticide resistance in Plutella xylostella by miRNA and lncRNA. |
title_short |
Regulation of GSTu1-mediated insecticide resistance in Plutella xylostella by miRNA and lncRNA. |
title_full |
Regulation of GSTu1-mediated insecticide resistance in Plutella xylostella by miRNA and lncRNA. |
title_fullStr |
Regulation of GSTu1-mediated insecticide resistance in Plutella xylostella by miRNA and lncRNA. |
title_full_unstemmed |
Regulation of GSTu1-mediated insecticide resistance in Plutella xylostella by miRNA and lncRNA. |
title_sort |
regulation of gstu1-mediated insecticide resistance in plutella xylostella by mirna and lncrna. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doaj.org/article/41352dccc1714721b185d971a9cf67c9 |
work_keys_str_mv |
AT binzhu regulationofgstu1mediatedinsecticideresistanceinplutellaxylostellabymirnaandlncrna AT linhongli regulationofgstu1mediatedinsecticideresistanceinplutellaxylostellabymirnaandlncrna AT ruiwei regulationofgstu1mediatedinsecticideresistanceinplutellaxylostellabymirnaandlncrna AT peiliang regulationofgstu1mediatedinsecticideresistanceinplutellaxylostellabymirnaandlncrna AT xiwugao regulationofgstu1mediatedinsecticideresistanceinplutellaxylostellabymirnaandlncrna |
_version_ |
1718375654767984640 |