Antibacterial activity of gold nanorods against Staphylococcus aureus and Propionibacterium acnes: misinterpretations and artifacts

Nouf N Mahmoud, Alaaldin M Alkilany, Enam A Khalil, Amal G Al-Bakri Department of Pharmaceutics and Pharmaceutical Technology, School of Pharmacy, The University of Jordan, Amman, Jordan Abstract: The antibacterial activity of gold nanorod (GNR) suspensions of different surface functionalities was...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mahmoud NN, Alkilany AM, Khalil EA, Al-Bakri AG
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Antibacterial activity supernatant gold nanorods colloidal stability artifacts acne vulgaris. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/41353ff83739405394eab114af142e97
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:41353ff83739405394eab114af142e97
record_format	dspace
spelling	oai:doaj.org-article:41353ff83739405394eab114af142e972021-12-02T07:31:58ZAntibacterial activity of gold nanorods against Staphylococcus aureus and Propionibacterium acnes: misinterpretations and artifacts1178-2013https://doaj.org/article/41353ff83739405394eab114af142e972017-10-01T00:00:00Zhttps://www.dovepress.com/antibacterial-activity-of-gold-nanorods-against-staphylococcus-aureus--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Nouf N Mahmoud, Alaaldin M Alkilany, Enam A Khalil, Amal G Al-Bakri Department of Pharmaceutics and Pharmaceutical Technology, School of Pharmacy, The University of Jordan, Amman, Jordan Abstract: The antibacterial activity of gold nanorod (GNR) suspensions of different surface functionalities was investigated against standard strains of Staphylococcus aureus and Propionibacterium acnes, taking into consideration two commonly "overlooked" factors: the colloidal stability of GNR suspensions upon mixing with bacterial growth media and the possible contribution of "impurities/molecules" in GNR suspensions to the observed antibacterial activity. The results demonstrated that cationic polyallylamine hydrochloride (PAH)-GNR were severely aggregated when exposed to bacterial growth media compared to other GNR suspensions. In addition, the free cetyltrimethylammonium bromide (CTAB) present in GNR suspensions is most likely the origin of the observed antibacterial activity. However, the antibacterial activity of GNR themselves could not be excluded. Probing these two critical control studies prevents misinterpretations and artifacts of the antibacterial activity of nanoparticles. Unfortunately, these practices are usually ignored in the published studies and may explain the significant conflicting results. In addition, this study indicates that GNR could be a promising candidate for the treatment of skin follicular diseases such as acne vulgaris. Keywords: antibacterial activity, supernatant, gold nanorods, colloidal stability, artifacts, acne vulgarisMahmoud NNAlkilany AMKhalil EAAl-Bakri AGDove Medical PressarticleAntibacterial activitysupernatantgold nanorodscolloidal stabilityartifactsacne vulgaris.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 7311-7322 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Antibacterial activity supernatant gold nanorods colloidal stability artifacts acne vulgaris. Medicine (General) R5-920
spellingShingle	Antibacterial activity supernatant gold nanorods colloidal stability artifacts acne vulgaris. Medicine (General) R5-920 Mahmoud NN Alkilany AM Khalil EA Al-Bakri AG Antibacterial activity of gold nanorods against Staphylococcus aureus and Propionibacterium acnes: misinterpretations and artifacts
description	Nouf N Mahmoud, Alaaldin M Alkilany, Enam A Khalil, Amal G Al-Bakri Department of Pharmaceutics and Pharmaceutical Technology, School of Pharmacy, The University of Jordan, Amman, Jordan Abstract: The antibacterial activity of gold nanorod (GNR) suspensions of different surface functionalities was investigated against standard strains of Staphylococcus aureus and Propionibacterium acnes, taking into consideration two commonly "overlooked" factors: the colloidal stability of GNR suspensions upon mixing with bacterial growth media and the possible contribution of "impurities/molecules" in GNR suspensions to the observed antibacterial activity. The results demonstrated that cationic polyallylamine hydrochloride (PAH)-GNR were severely aggregated when exposed to bacterial growth media compared to other GNR suspensions. In addition, the free cetyltrimethylammonium bromide (CTAB) present in GNR suspensions is most likely the origin of the observed antibacterial activity. However, the antibacterial activity of GNR themselves could not be excluded. Probing these two critical control studies prevents misinterpretations and artifacts of the antibacterial activity of nanoparticles. Unfortunately, these practices are usually ignored in the published studies and may explain the significant conflicting results. In addition, this study indicates that GNR could be a promising candidate for the treatment of skin follicular diseases such as acne vulgaris. Keywords: antibacterial activity, supernatant, gold nanorods, colloidal stability, artifacts, acne vulgaris
format	article
author	Mahmoud NN Alkilany AM Khalil EA Al-Bakri AG
author_facet	Mahmoud NN Alkilany AM Khalil EA Al-Bakri AG
author_sort	Mahmoud NN
title	Antibacterial activity of gold nanorods against Staphylococcus aureus and Propionibacterium acnes: misinterpretations and artifacts
title_short	Antibacterial activity of gold nanorods against Staphylococcus aureus and Propionibacterium acnes: misinterpretations and artifacts
title_full	Antibacterial activity of gold nanorods against Staphylococcus aureus and Propionibacterium acnes: misinterpretations and artifacts
title_fullStr	Antibacterial activity of gold nanorods against Staphylococcus aureus and Propionibacterium acnes: misinterpretations and artifacts
title_full_unstemmed	Antibacterial activity of gold nanorods against Staphylococcus aureus and Propionibacterium acnes: misinterpretations and artifacts
title_sort	antibacterial activity of gold nanorods against staphylococcus aureus and propionibacterium acnes: misinterpretations and artifacts
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/41353ff83739405394eab114af142e97
work_keys_str_mv	AT mahmoudnn antibacterialactivityofgoldnanorodsagainststaphylococcusaureusandpropionibacteriumacnesmisinterpretationsandartifacts AT alkilanyam antibacterialactivityofgoldnanorodsagainststaphylococcusaureusandpropionibacteriumacnesmisinterpretationsandartifacts AT khalilea antibacterialactivityofgoldnanorodsagainststaphylococcusaureusandpropionibacteriumacnesmisinterpretationsandartifacts AT albakriag antibacterialactivityofgoldnanorodsagainststaphylococcusaureusandpropionibacteriumacnesmisinterpretationsandartifacts
_version_	1718399332475994112

Antibacterial activity of gold nanorods against Staphylococcus aureus and Propionibacterium acnes: misinterpretations and artifacts

Ejemplares similares