Silenced SNHG1 Inhibited Epithelial-Mesenchymal Transition and Inflammatory Response of ARPE-19 Cells Induced by High Glucose

Jing Yang,1 Kun Yang,2 Xuxia Meng,1 Penghui Liu,1 Yudong Fu,1 Yibo Wang1 1Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao, 266500, Shandong Province, People’s Republic of China; 2Central Laboratory, Affiliated Hospital of Qingdao University, Qingdao, 266500, Shandong Prov...

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Autores principales:	Yang J, Yang K, Meng X, Liu P, Fu Y, Wang Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	epithelial-mesenchymal transition small nucleolar rna host gene 1 retinal pigment epithelial hyperglycemia Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/4136b14ecef440428e1d2a25ccb455c1
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spelling	oai:doaj.org-article:4136b14ecef440428e1d2a25ccb455c12021-12-02T14:28:07ZSilenced SNHG1 Inhibited Epithelial-Mesenchymal Transition and Inflammatory Response of ARPE-19 Cells Induced by High Glucose1178-7031https://doaj.org/article/4136b14ecef440428e1d2a25ccb455c12021-04-01T00:00:00Zhttps://www.dovepress.com/silenced-snhg1-inhibited-epithelial-mesenchymal-transition-and-inflamm-peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Jing Yang,1 Kun Yang,2 Xuxia Meng,1 Penghui Liu,1 Yudong Fu,1 Yibo Wang1 1Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao, 266500, Shandong Province, People’s Republic of China; 2Central Laboratory, Affiliated Hospital of Qingdao University, Qingdao, 266500, Shandong Province, People’s Republic of ChinaCorrespondence: Xuxia Meng Email mengxx@qduhospital.cnPurpose: The lncRNA small nucleolar RNA host gene 1 (SNHG1) is a cerebral infarction-associated gene, its biological role and mechanism in diabetic retinopathy remain to be illuminated. The present study was designed to investigate the role of SNHG1 in high glucose induced human retinal pigment epithelial cells (ARPE-19).Methods: ARPE-19 cells were cultured and exposed to 60 mM high glucose for 48h, and 5.5mM glucose-exposed ARPE-19 cells were used as the control. The levels of the epithelial-mesenchymal transition (EMT) markers E-cadherin, ZO-1, vimentin and α-SMA were measured, and the Cell inflammatory response was evaluated by detecting IL-6 and IL-1β levels. Then, cell migration, proliferation and apoptosis were detected. The expression of the lncRNA SNHG1 in ARPE-19 cells was detected by quantitative real-time PCR. SNHG1 was knocked down by small interfering RNA (siRNA) transfection. The effects of SNHG1 inhibition on inflammation, EMT, migration, proliferation and apoptosis were observed.Results: The results showed that the expression of SNHG1 was significantly increased in ARPE-19 cells exposed to high glucose. Silencing SNHG1 reduced the expression of vimentin, α-SMA, and the expression of inflammatory chemokines IL-6 and IL-1β, inhibited migration and proliferation, elevated the expression of E-cadherin and ZO-1, and promoted apoptosis in ARPE-19 cells.Conclusion: The lncRNA SNHG1 is involved in hyperglycemia-induced EMT and the inflammatory response of ARPE-19 cells and provides a new understanding of the pathogenesis of DR.Keywords: epithelial-mesenchymal transition, small nucleolar RNA host gene 1, retinal pigment epithelial, hyperglycemiaYang JYang KMeng XLiu PFu YWang YDove Medical Pressarticleepithelial-mesenchymal transitionsmall nucleolar rna host gene 1retinal pigment epithelialhyperglycemiaPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 1563-1573 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	epithelial-mesenchymal transition small nucleolar rna host gene 1 retinal pigment epithelial hyperglycemia Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	epithelial-mesenchymal transition small nucleolar rna host gene 1 retinal pigment epithelial hyperglycemia Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Yang J Yang K Meng X Liu P Fu Y Wang Y Silenced SNHG1 Inhibited Epithelial-Mesenchymal Transition and Inflammatory Response of ARPE-19 Cells Induced by High Glucose
description	Jing Yang,1 Kun Yang,2 Xuxia Meng,1 Penghui Liu,1 Yudong Fu,1 Yibo Wang1 1Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao, 266500, Shandong Province, People’s Republic of China; 2Central Laboratory, Affiliated Hospital of Qingdao University, Qingdao, 266500, Shandong Province, People’s Republic of ChinaCorrespondence: Xuxia Meng Email mengxx@qduhospital.cnPurpose: The lncRNA small nucleolar RNA host gene 1 (SNHG1) is a cerebral infarction-associated gene, its biological role and mechanism in diabetic retinopathy remain to be illuminated. The present study was designed to investigate the role of SNHG1 in high glucose induced human retinal pigment epithelial cells (ARPE-19).Methods: ARPE-19 cells were cultured and exposed to 60 mM high glucose for 48h, and 5.5mM glucose-exposed ARPE-19 cells were used as the control. The levels of the epithelial-mesenchymal transition (EMT) markers E-cadherin, ZO-1, vimentin and α-SMA were measured, and the Cell inflammatory response was evaluated by detecting IL-6 and IL-1β levels. Then, cell migration, proliferation and apoptosis were detected. The expression of the lncRNA SNHG1 in ARPE-19 cells was detected by quantitative real-time PCR. SNHG1 was knocked down by small interfering RNA (siRNA) transfection. The effects of SNHG1 inhibition on inflammation, EMT, migration, proliferation and apoptosis were observed.Results: The results showed that the expression of SNHG1 was significantly increased in ARPE-19 cells exposed to high glucose. Silencing SNHG1 reduced the expression of vimentin, α-SMA, and the expression of inflammatory chemokines IL-6 and IL-1β, inhibited migration and proliferation, elevated the expression of E-cadherin and ZO-1, and promoted apoptosis in ARPE-19 cells.Conclusion: The lncRNA SNHG1 is involved in hyperglycemia-induced EMT and the inflammatory response of ARPE-19 cells and provides a new understanding of the pathogenesis of DR.Keywords: epithelial-mesenchymal transition, small nucleolar RNA host gene 1, retinal pigment epithelial, hyperglycemia
format	article
author	Yang J Yang K Meng X Liu P Fu Y Wang Y
author_facet	Yang J Yang K Meng X Liu P Fu Y Wang Y
author_sort	Yang J
title	Silenced SNHG1 Inhibited Epithelial-Mesenchymal Transition and Inflammatory Response of ARPE-19 Cells Induced by High Glucose
title_short	Silenced SNHG1 Inhibited Epithelial-Mesenchymal Transition and Inflammatory Response of ARPE-19 Cells Induced by High Glucose
title_full	Silenced SNHG1 Inhibited Epithelial-Mesenchymal Transition and Inflammatory Response of ARPE-19 Cells Induced by High Glucose
title_fullStr	Silenced SNHG1 Inhibited Epithelial-Mesenchymal Transition and Inflammatory Response of ARPE-19 Cells Induced by High Glucose
title_full_unstemmed	Silenced SNHG1 Inhibited Epithelial-Mesenchymal Transition and Inflammatory Response of ARPE-19 Cells Induced by High Glucose
title_sort	silenced snhg1 inhibited epithelial-mesenchymal transition and inflammatory response of arpe-19 cells induced by high glucose
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/4136b14ecef440428e1d2a25ccb455c1
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_version_	1718391247418163200

Silenced SNHG1 Inhibited Epithelial-Mesenchymal Transition and Inflammatory Response of ARPE-19 Cells Induced by High Glucose

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