KDNA genetic signatures obtained by LSSP-PCR analysis of Leishmania (Leishmania) infantum isolated from the new and the old world.

<h4>Background</h4>Visceral Leishmaniasis (VL) caused by species from the Leishmania donovani complex is the most severe form of the disease, lethal if untreated. VL caused by Leishmania infantum is a zoonosis with an increasing number of human cases and millions of dogs infected in the...

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Autores principales:	Janaína Sousa Campos Alvarenga, Carla Maia Ligeiro, Célia Maria Ferreira Gontijo, Sofia Cortes, Lenea Campino, Annamaria Ravara Vago, Maria Norma Melo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/414563541b444a088703eb76755ae0f6
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spelling	oai:doaj.org-article:414563541b444a088703eb76755ae0f62021-11-18T07:08:22ZKDNA genetic signatures obtained by LSSP-PCR analysis of Leishmania (Leishmania) infantum isolated from the new and the old world.1932-620310.1371/journal.pone.0043363https://doaj.org/article/414563541b444a088703eb76755ae0f62012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22912862/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Visceral Leishmaniasis (VL) caused by species from the Leishmania donovani complex is the most severe form of the disease, lethal if untreated. VL caused by Leishmania infantum is a zoonosis with an increasing number of human cases and millions of dogs infected in the Old and the New World. In this study, L. infantum (syn. L.chagasi) strains were isolated from human and canine VL cases. The strains were obtained from endemic areas from Brazil and Portugal and their genetic polymorphism was ascertained using the LSSP-PCR (Low-Stringency Single Specific Primer PCR) technique for analyzing the kinetoplastid DNA (kDNA) minicircles hypervariable region.<h4>Principal findings</h4>KDNA genetic signatures obtained by minicircle LSSP-PCR analysis of forty L. infantum strains allowed the grouping of strains in several clades. Furthermore, LSSP-PCR profiles of L. infantum subpopulations were closely related to the host origin (human or canine). To our knowledge this is the first study which used this technique to compare genetic polymorphisms among strains of L. infantum originated from both the Old and the New World.<h4>Conclusions</h4>LSSP-PCR profiles obtained by analysis of L. infantum kDNA hypervariable region of parasites isolated from human cases and infected dogs from Brazil and Portugal exhibited a genetic correlation among isolates originated from the same reservoir, human or canine. However, no association has been detected among the kDNA signatures and the geographical origin of L. infantum strains.Janaína Sousa Campos AlvarengaCarla Maia LigeiroCélia Maria Ferreira GontijoSofia CortesLenea CampinoAnnamaria Ravara VagoMaria Norma MeloPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e43363 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Janaína Sousa Campos Alvarenga Carla Maia Ligeiro Célia Maria Ferreira Gontijo Sofia Cortes Lenea Campino Annamaria Ravara Vago Maria Norma Melo KDNA genetic signatures obtained by LSSP-PCR analysis of Leishmania (Leishmania) infantum isolated from the new and the old world.
description	<h4>Background</h4>Visceral Leishmaniasis (VL) caused by species from the Leishmania donovani complex is the most severe form of the disease, lethal if untreated. VL caused by Leishmania infantum is a zoonosis with an increasing number of human cases and millions of dogs infected in the Old and the New World. In this study, L. infantum (syn. L.chagasi) strains were isolated from human and canine VL cases. The strains were obtained from endemic areas from Brazil and Portugal and their genetic polymorphism was ascertained using the LSSP-PCR (Low-Stringency Single Specific Primer PCR) technique for analyzing the kinetoplastid DNA (kDNA) minicircles hypervariable region.<h4>Principal findings</h4>KDNA genetic signatures obtained by minicircle LSSP-PCR analysis of forty L. infantum strains allowed the grouping of strains in several clades. Furthermore, LSSP-PCR profiles of L. infantum subpopulations were closely related to the host origin (human or canine). To our knowledge this is the first study which used this technique to compare genetic polymorphisms among strains of L. infantum originated from both the Old and the New World.<h4>Conclusions</h4>LSSP-PCR profiles obtained by analysis of L. infantum kDNA hypervariable region of parasites isolated from human cases and infected dogs from Brazil and Portugal exhibited a genetic correlation among isolates originated from the same reservoir, human or canine. However, no association has been detected among the kDNA signatures and the geographical origin of L. infantum strains.
format	article
author	Janaína Sousa Campos Alvarenga Carla Maia Ligeiro Célia Maria Ferreira Gontijo Sofia Cortes Lenea Campino Annamaria Ravara Vago Maria Norma Melo
author_facet	Janaína Sousa Campos Alvarenga Carla Maia Ligeiro Célia Maria Ferreira Gontijo Sofia Cortes Lenea Campino Annamaria Ravara Vago Maria Norma Melo
author_sort	Janaína Sousa Campos Alvarenga
title	KDNA genetic signatures obtained by LSSP-PCR analysis of Leishmania (Leishmania) infantum isolated from the new and the old world.
title_short	KDNA genetic signatures obtained by LSSP-PCR analysis of Leishmania (Leishmania) infantum isolated from the new and the old world.
title_full	KDNA genetic signatures obtained by LSSP-PCR analysis of Leishmania (Leishmania) infantum isolated from the new and the old world.
title_fullStr	KDNA genetic signatures obtained by LSSP-PCR analysis of Leishmania (Leishmania) infantum isolated from the new and the old world.
title_full_unstemmed	KDNA genetic signatures obtained by LSSP-PCR analysis of Leishmania (Leishmania) infantum isolated from the new and the old world.
title_sort	kdna genetic signatures obtained by lssp-pcr analysis of leishmania (leishmania) infantum isolated from the new and the old world.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/414563541b444a088703eb76755ae0f6
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KDNA genetic signatures obtained by LSSP-PCR analysis of Leishmania (Leishmania) infantum isolated from the new and the old world.

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